PLK4 Antibody

Western Blot: PLK4 Antibody [NB100-894] - Analysis of PLK4 in human Colon lysate (35ug protein in RIPA buffer) using NB100-894 at 2 ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunocytochemistry/Immunofluorescence: PLK4 Antibody [NB100-894] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: PLK4 Antibody [NB100-894] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: PLK4 Antibody [NB100-894] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Product Discontinued

PLK4 Antibody Summary

• Immunogen: Peptide with sequence ATCIGEKIEDFKVGN corresponding to N-Terminus according to NP_055079.3.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10ug/ml
  • Immunocytochemistry/ Immunofluorescence 10ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human colon lysates (calculated MW of 109 kDa band according to NP_055079.3).
• Theoretical MW: 109 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Placenta Whole Tissue Lysate (Adult Whole Normal)
  PLK4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLK4 Antibody

• EC 2.7.11.21
• PLK4
• PLK-4
• polo-like kinase 4 (Drosophila)
• Polo-like kinase 4
• polo-like kinase 4SAKSTK18serine/threonine kinase 18
• SAK
• serine/threonine protein kinase SAK
• Serine/threonine-protein kinase 18
• serine/threonine-protein kinase PLK4
• Serine/threonine-protein kinase Sak
• Snk akin kinase
• STK18

Background

PLK4 is involved in regulation of cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLK4 Antibody (NB100-894)(5)

Publications using NB100-894

• Guerber L, Vuidel A, Liao Y et al. UBAP2L-dependent coupling of PLK1 localization and stability during mitosis EMBO reports 2023-06-05 [PMID: 37039032] (WB, Human)
• Kazazian K, Haffani Y, Ng D et al. FAM46C/TENT5C functions as a tumor suppressor through inhibition of Plk4 activity Commun Biol 2020-08-17 [PMID: 32807875] (ICC/IF, Human)
• So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 2019-06-28 [PMID: 31249032] (ICC/IF, Sheep, Bovine, Mouse, Porcine)
• Rizki, A et al. Polo-like Kinase 1 Is Involved in Invasion through Extracellular Matrix. Cancer Res67:11106-11110. 2007-01-01 [PMID: 18056432]
• Hudson JW, Chen L, Fode C et al. Sak kinase gene structure and transcriptional regulation. Gene 2000-01-04 [PMID: 10607900]

Bioinformatics

• Gene Symbol: PLK4
• Entrez:
  • 10733(Human)
• Uniprot:
  • O00444(Human)