Recombinant PreScission Protease Protein

SDS-Page: Recombinant Viral PreScission Protease Protein [NBP2-61442] - Recombinant Viral TEV Protease Protein [NBP2-61442]

• Applications: PAGE, Enzyme Activity

Recombinant PreScission Protease Protein Summary

• Description: PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease. rPP is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease.
  
  One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90 % completion at 5 °C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
  
  

  Source: E. coli

• Specificity: The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
• Details of Functionality: The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Application Notes: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. It works most effective at 4C but can digest substrates at room temperature. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20 or NonidetTM P40 to a concentration of 0.01 %. Concentrations of these detergents up to 1 % do not inhibit PreScission Protease.
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
• Preservative: No Preservative
• Purity: Peptide affinity purified

Alternate Names for Recombinant PreScission Protease Protein

• 3C protease
• Picornain 3C
• PSP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.