PRMT5 Antibody (7-C2)

Western Blot: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of PRMT5 on mouse kidney lysates using anti-PRMT5 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of paraffin-embedded rat liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of paraffin-embedded human liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PRMT5 Antibody (7-C2) [NBP2-67075] - Analysis of paraffin-embedded rat kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

Product Discontinued

PRMT5 Antibody (7-C2) Summary

• Immunogen: Synthetic peptide within Human PRMT5 aa 1-50 / 637. (SwissProt: O14744 Human)
• Localization: Nucleus, cytoplasm.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRMT5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:1000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for PRMT5 Antibody (7-C2)

• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.320
• Histone-arginine N-methyltransferase PRMT5
• HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein
• HRMT1L5
• IBP72
• Jak-binding protein 1
• JBP1
• PRMT5
• protein arginine methyltransferase 5
• protein arginine N-methyltransferase 5
• Shk1 kinase-binding protein 1 homolog
• skb1 (S. pombe) homolog
• SKB1 homolog (S. pombe)
• SKB1 homolog
• SKB1
• SKB1HsSKB1 homolog

Background

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT5