PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free

Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry/Immunofluorescence: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C136 (NBP3-20131) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20131, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, ChIP, MS
• Clone: RAB-C136
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT5 protein under non-denaturing conditions.
• Epitope: This antibody binds to a folded domain, amino acids 269-637.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRMT5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Mass Spectrometry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free

• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.320
• Histone-arginine N-methyltransferase PRMT5
• HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein
• HRMT1L5
• IBP72
• Jak-binding protein 1
• JBP1
• PRMT5
• protein arginine methyltransferase 5
• protein arginine N-methyltransferase 5
• Shk1 kinase-binding protein 1 homolog
• skb1 (S. pombe) homolog
• SKB1 homolog (S. pombe)
• SKB1 homolog
• SKB1
• SKB1HsSKB1 homolog

Background

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT5