ProDots Recombinant Human FGF-10 Protein

ProDots® Recombinant Human FGF-10 stimulated cell proliferation of the 4MBr-5 rhesus monkey epithelial cell line. The ED50 for this effect is 20-100 ng/mL.

1 μg/lane of ProDots® Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 kDa.

Product Discontinued

ProDots Recombinant Human FGF-10 Protein Summary

• Additional Information: Supplied as ready-to-use 5 ug protein balls
• Details of Functionality: Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED₅₀ for this effect is 20-100 ng/mL.
• Source: E. coli-derived human FGF-10 protein
  Cys37 - Ser208 & Gly41 - Ser208
• Accession #: O15520
• N-terminal Sequence: Cys37 & Gly41
• Protein/Peptide Type: ProDots Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature.
  • 12 months from date of receipt at 2-8 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use.

Notes

726884.0

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ProDots Recombinant Human FGF-10 Protein

• FGF10
• FGF-10
• fibroblast growth factor 10
• Keratinocyte growth factor 2
• KGF2
• KGF-2
• produced by fibroblasts of urinary bladder lamina propria

Background

FGF-10 (Fibroblast Growth Factor 10) is a heparin binding glycoprotein in the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in limb bud initiation, palate development, branching morphogenesis and directional outgrowth of lung buds, formation of the otic vesicle and chochlea, adipogenesis, and the development of prostate, mammary, lacrimal, and submandibular salivary glands. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF-10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer.

Bioinformatics

• Uniprot:
  • O15520()