ProDots Recombinant Human G-CSF Protein

ProDots® Recombinant Human G-CSF stimulated cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.

1 μg/lane of ProDots® Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at approximately 18 kDa.

Product Discontinued

ProDots Recombinant Human G-CSF Protein Summary

• Additional Information: Supplied as ready-to-use 5 ug protein balls
• Details of Functionality: Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED₅₀ for this effect is 10-60 pg/mL.
• Source: E. coli-derived human G-CSF protein
  Thr31 - Pro204, with an -N-terminal Met
• Accession #: NP_757373
• N-terminal Sequence: Met
• Protein/Peptide Type: ProDots Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature.
  • 12 months from date of receipt at 2-8 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot^® directly into the cell culture medium for immediate use.

Notes

726879.0

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ProDots Recombinant Human G-CSF Protein

• C17orf33
• chromosome 17 open reading frame 33
• colony stimulating factor 3 (granulocyte)
• CSF3
• CSF3OS
• Filgrastim
• GCSF
• G-CSF
• GCSFlenograstim
• granulocyte colony-stimulating factor
• Lenograstim
• MGC45931
• Pluripoietin

Background

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

Bioinformatics

• Uniprot:
  • NP_757373()