Recombinant Human Proinsulin C-Peptide Analogue Protein

SDS-Page: Human Proinsulin C-Peptide Analogue Protein [NBP2-35211]

• Reactivity: Hu
• Applications: WB, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Proinsulin C-Peptide Analogue Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 35 amino acids corresponding to Proinsulin C-Peptide Analogue Source: E. coli

  Uniprot ID: P01308

  Amino Acid Sequence: RREAEDLQVG QVELGGGPGA GSLQPLALEG SLQKR
  
  A single non-glycosylated polypeptide chain containing 35 amino acids corresponding to Proinsulin C-Peptide Analogue Source: E. coli

  Uniprot ID: P01308

  Amino Acid Sequence: RREAEDLQVG QVELGGGPGA GSLQPLALEG SLQKR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: INS
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Proinsulin C-Peptide Analogue as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Application Notes: Use in WB reported in secitific publication PMID: 32433667
• Theoretical MW: 3.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in secitific publication PMID: 32433667

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Background

Proinsulin, a molecular weight of 8 kDa to 10 kDa, is a precursor of insulin. It has minimum hormonal activity and is converted to insulin by removal of the connecting C-peptide. Mature insulin has 35 fewer amino acids; 4 are removed altogether and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence; the two other ends (the B chain and A chain) remain connected by disulfide bonds. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle and glycogen synthesis in liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Proinsulin C-Peptide Analogue Recombinant Protein (NBP2-35211)(2)

Publications using NBP2-35211

• Lin X, Wang G, Ma L, Liu G Study on Factors Affecting the Performance of a CRISPR/Cas-Assisted New Immunoassay: Detection of Salivary Insulin as an Example Frontiers in Bioengineering and Biotechnology 2021-11-11 [PMID: 34858958]
• Viviano J, Brecker M, Ferrara-Cook C et al. ERp29 as a regulator of Insulin biosynthesis PLoS ONE 2020-05-20 [PMID: 32433667] (WB, Mouse)

Bioinformatics

• Gene Symbol: INS
• Uniprot:
  • P01308()
  • P01308()
  • P01308()