Recombinant Protein A/G Protein

SDS-Page: Protein A/G Protein [NBP2-34968]

• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Protein A/G Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 429 amino acids corresponding to Protein A/G Source: E. coli

  Amino Acid Sequence: NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTE
  
  This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

• Specificity: Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.
• Details of Functionality: Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from Protein A/G to ensure the maximum specific IgG binding. Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. It binds to various human, mouse and rat IgG subclasses. It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Protein A/G as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 47.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from additive free solution.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Dissolve in distilled water or saline.

Background

The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C1C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Protein A/G Recombinant Protein (NBP2-34968). (Showing 1 - 1 of 1 FAQ).

1. Do you have any information on binding sites for your Recombinant Protein A/G Protein (NBP2-34968)?
   • This is a product from one of our sister brands and their lab has just cloned the protein chimera but no further characterization studies were performed. My advice for you would be to search available literature on Protein and G binding sites characterization.  Since this Protein AG has all binding sites from both A and G proteins I think the initial characterization of these proteins should apply. I've tried to find the answer for you and the most relevant data I was able to find comes from Moks et al. 1986 publication (Eur. J. Biochem. 1986. 156,637-643). Basically, the sequences of the binding sites are highly homologous: ABC are identical and D and E differ in 2-3 aminoacids (Fig1). That gives the first clue that the binding specificity will likely be very similar. Later on they show the binding capacity of sites E and B, concluding that the site E has lower binding affinity than B, and they postulate that that the differences in binding affinity of sites A, B, C and D may exist, however are not great. To my understanding it is enough to conclude (assuming the same is true for Protein G) that all sites will be able to bind the same type of IgG, although with somewhat different affinity. There is however an earlier paper that may be of interest to you - Langone et al.  1978 (J Immunol 1978; 121:333-338; ;). These researchers report that intact Protein A, despite having 4 binding sites (now we now there are actually 5), can only bind 2 molecules of IgG.