Recombinant Protein G Protein [Biotin]

Product Discontinued

Recombinant Protein G Protein [Biotin] Summary

• Description: A recombinant protein corresponding to non species specific Protein G
• Preparation Method: >98% SDS-PAGE
• Details of Functionality: Binding Capacity: Under optimal conditions, 1 mg protein G will bind approximately 5 mg human IgG. Optimal binding of Protein G to antibodies occurs at pH 5.0 to 6.0 and can be eluted over a pH range of 2.5 to 3.0.
• Protein/Peptide Type: Recombinant Protein
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Theoretical MW: 26.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: No additives.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with water to a final concentration of 5.0 mg/ml.

Notes

After reconstituted, solution is clear.

Alternate Names for Recombinant Protein G Protein [Biotin]

• IgG binding protein G Immunoglobulin G binding protein G [Precursor] spg

Background

Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. 's Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.The Protein G-Biotin is using purified recombinant protein G biotinylated with ~2 biotins per protein G molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.