Recombinant Protein G Cys (N-Term) Protein

• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Protein G Cys (N-Term) Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 201 amino acids corresponding to Protein G with a Cys on the N-terminus. However it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE Source: E. coli

  Uniprot ID: P19909

  Amino Acid Sequence: CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E

• Specificity: Protein G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and rabbit.
• Details of Functionality: Protein G is a genetically engineered protein containing 3 IgG-binding regions of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein G binds to various human, mouse and rat IgG subclasses. It also binds to total IgG from cow, goat, sheep, house and rabbit.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Protein G as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 21.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from additive free solution.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Dissolve in distilled water or saline.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Protein G Cys (N-Term) Protein

• IgG binding protein G Immunoglobulin G binding protein G [Precursor] spg

Background

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Protein G Recombinant Protein (NBP2-34962)(3)

Publications using NBP2-34962

• Matthias Paul, Harald Kühnel, Rudolf Oberpertinger, Christoph Mehofer, Doris Pollhammer, Markus Wellenzohn, Alfredo de la Escosura-Muñiz Two-Layer Inkjet-Printed Microwave Split-Ring Resonators for Detecting Analyte Binding to the Gold Surface Sensors (Basel, Switzerland) 2024-03-06 [PMID: 38475224]
• Zhichao Lou, Jinfeng Wan, Xiaohong Zhang, HaiqianZhang. Quick and sensitive SPR detection of prion disease-associated isoform (PrP Sc) based on its self-assembling behavior on bare gold film and specific interactions with aptamer-graphene oxide (AGO). Colloids Surf B Biointerfaces 2017-09-01 [PMID: 28570989]
• Solodka K, Berto M, Ferraro D et al. Detection of Neurofilament Light Chain with Label Free Electrolyte Gated Organic Field Effect Transistors Advanced Materials Interfaces 2022-02-10

FAQs for Protein G Recombinant Protein (NBP2-34962). (Showing 1 - 1 of 1 FAQs).

1. I was looking at the recombinant Protein G (NBP2-34962) and wanted to confirm that it does not contain a His tag
   • I believe this protein does not have a histidine tag but it does have a CYS at the N-term.