PTEN Antibody (28H6)

Product Discontinued

PTEN Antibody (28H6) Summary

• Immunogen: BALB/C mice were injected with prokaryotic recombinant protein corresponding to a 200 amino acid C-terminal region of the PTEN molecule.
• Localization: Nuclear
• Specificity: This antibody reacts with human PTEN also known as MMAC or TEP1. PTEN is a tumor suppressor gene, which encodes a multifunctional phosphatase and which is expressed almost ubiquitously and regulates the cell cycle, apoptosis, and cell adhesion. Deletions and mutations of PTEN occur in a range of cancers including breast cancer, malignant melanoma, endometrial carcinoma, bladder carcinoma, small cell lung carcinoma, and endometrial ovarian carcinoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTEN
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This antibody is useful in Immunohistochemistry-Paraffin. This antibody may be diluted to a titer of 1:50-1:75 in an ABC method.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for PTEN Antibody (28H6)

• BZS
• EC 3.1.3.16,10q23del
• EC 3.1.3.48
• EC 3.1.3.67
• GLM2
• MMAC1 phosphatase and tensin homolog deleted on chromosome 10
• MMAC1
• MMAC1MGC11227
• Mutated in multiple advanced cancers 1
• phosphatase and tensin homologDEC
• phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN
• PTEN
• PTEN1
• TEP1MHAM

Background

The PTEN tumor suppressor gene acts as a phospholipid phosphatase. Under normal growth conditions, stimulatory signals from the insulin receptor activate the enzyme PI3-K, which phosphorylates phosphotidylinositol 4,5-bisphosphate (PIP2) to generate phosphotidylinositol 3,4,5-triphosphate (PIP3), a lipid molecule. Downstream, PIP3 activates several effectors, including the proto-oncogene product PKB/Akt. The role of PTEN is to dephosphorylate PIP3, acting as a negative control on PKB/Akt activation. If a mutation in PTEN causes it unable to carry out its phosphatase function, PIP3 can no longer be deactivated, so it continues to propagate its signal downstream. This may result in the continued activation of PKB/Akt, which, in combination with other factors, could lead to increased cell growth and possible tumor development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTEN Antibody (NB200-175)(3)

Publications using NB200-175

• Eritja N, Santacana M, Maiques O et al. Modeling glands with PTEn deficient cells and microscopic methods for assessing PTEn loss: Endometrial cancer as a model. Methods. 2014-11-18 [PMID: 25461816]
• Carvalho Katia C, Maia Beatriz M, Omae Samantha V et al. Best practice for PTEN gene and protein assessment in anatomic pathology. Acta Histochem. 2014-01-01 [PMID: 23746542] (IHC-P, Human)
• Neto JC, Ikoma MM, Carvalho KC et al. MGMT and PTEN as potential prognostic markers in breast cancer. Experimental and Molecular Pathology;92(1):20-26. 2011-10-12 [PMID: 22019339]

FAQs for PTEN Antibody (NB200-175). (Showing 1 - 1 of 1 FAQs).

1. I would like to know if these antibodies are suitable with IHC techniques using HRP conjugated antibodies and DAB as chromogen? Cat# NB200-175, NB100-904 and NB100-142
   • Yes, all of these antibodies are useful for IHC-P with DAB based detection. Please note that the PTEN Antibody (28H6) NB200-175 has been discontinued, however.

Bioinformatics

• Gene Symbol: PTEN