Purple Hematoxylin Solution

Immunohistochemistry-Paraffin: Purple Hematoxylin Solution [NBP3-12176] - Formalin fixed paraffin embedded human colon stained with epithelial antigen antibody labeled with Purple Hematoxylin Solution (NBP3-12176)

• Applications: IHC

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Preparation of Working Solutions
  
  Purple Hematoxylin is ready to use and does not require any preparation.
  
  Protocol Recommendations
  
  Counterstain
  1. Rinse slides with deionized or distilled water.
  2. Place slides into Purple Hematoxylin for 30-60 seconds.
  3. Rinse slides in deionized or distilled water until all free hematoxylin has been removed.
  4. Bluing the slides in an alkaline solution is not required and is not recommended.

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Background

Purple Hematoxylin Solution is intended for use in the histologic demonstration of nuclear staining. This staining technique is used to make the critical distinction between a normal nucleus and an abnormal one.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.