Rab5a Antibody (ST47-03)

Western Blot: Rab5a Antibody (ST47-03) [NBP2-67502] - Analysis of Rab5 on different lysates using anti-Rab5 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7

Immunohistochemistry-Paraffin: Rab5a Antibody (ST47-03) [NBP2-67502] - Analysis of paraffin-embedded mouse prostate tissue using anti-Rab5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rab5a Antibody (ST47-03) [NBP2-67502] - Analysis of paraffin-embedded mouse brain tissue using anti-Rab5 antibody. Counter stained with hematoxylin.

Product Discontinued

Rab5a Antibody (ST47-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Rab5a aa 166-215 / 215. (SwissProt: P20339 Human; SwissProt: Q9CQD1 Mouse; SwissProt: M0RC99 Rat)
• Localization: Cell membrane, Cytoplasm, Cell projection, Membrane, Endosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB5A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rab5a Antibody (ST47-03)

• RAB5A, member RAS oncogene family
• RAB5RAS-associated protein RAB5A
• ras-related protein Rab-5A

Background

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5 -triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB5A