Rad21 Antibody (CM110-2C10) - BSA Free

Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.

Simple Western: Rad21 Antibody (CM110-2C10) [NB100-386] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western
• Clone: CM110-2C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Rad21 Antibody (CM110-2C10) - BSA Free Summary

• Immunogen: An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
• Localization: Nuclear and cytoplasm
• Specificity: NB100-386 is specific for both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD21
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:50
  • Western Blot 1:500 (ECL)
• Application Notes: This Rad21 (CM110-2C10) antibody can be used in Western blot, where two bands are seen. The full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 72 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa Whole Cell Lysate
  Rad21 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Rad21 Antibody (CM110-2C10) - BSA Free

• double-strand-break repair protein rad21 homolog
• hHR21FLJ25655
• HR21
• KIAA0078RAD21 (S. pombe) homolog
• MCD1
• Nuclear matrix protein 1
• NXP-1
• NXP1HRAD21
• protein involved in DNA double-strand break repair
• RAD21 homolog (S. pombe)
• SCC1 homolog
• SCC1FLJ40596

Background

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad21 Antibody (NB100-386)(1)

Publication using NB100-386

• Schiapparelli LM, Xie Y, Sharma P et al. Activity-Induced Cortical Glutamatergic Neuron Nascent Proteins The Journal of neuroscience : the official journal of the Society for Neuroscience 2022-10-19 [PMID: 36261270] (WB, Mouse)

Bioinformatics

• Gene Symbol: RAD21
• Entrez:
  • 5885(Human)
  • 19357(Mouse)
• Uniprot:
  • O60216(Human)