Rad51 Antibody (14B4) - Azide and BSA Free

Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY license.

Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - HCC1937 (human immortalized breast cancer) cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.

Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa whole cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.

Western Blot: Rad51 Antibody (14B4) [NB100-148] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4].

Western Blot: Rad51 Antibody (14B4) [NB100-148] - YFP-PALB2 & YFP-PALB2 146AAAA binds RAD51 equally.Lysates from HEK293T cells expressing the indicated constructs were subjected to GFP-Trap pulldown & immunoblotting against YFP or RAD51. YFP alone was used as a negative control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31017574), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Bractoppin Selectively Interrupts BRCA1-Dependent Steps in DNA Repair by Homologous Recombination(A) Confocal images depicting at high magnification the recruitment of the RPA32 protein into nuclear foci after the indicated treatments. Experiments were carried out as described as in Figure 5A. Staining in the upper row is for RPA32 (green), middle row, for mCherry-BRCA1 tBRCT (red); lower row, merged red & green staining, with DNA staining (DAPI) in blue. Scale bar represents 10 μm.(B) Recruitment of RAD51 protein into nuclear foci, measured & depicted as described in (A). Scale bar represents 10 μm.(C) Percentage of cells positive for radiation-induced nuclear RPA32 foci (mean ± SD; n = 5,300, 0 Gy; 3,200, 16 Gy; 3,600, BRCA1 tBRCT; 3,400, Bractoppin; 3,500, CCBT2047) enumerated by high-content imaging (see the STAR Methods). Statistical significance was performed using an unpaired two-tailed t test. ***p ≤ 0.001. Similar results were observed in three independent repeats.(D) Percentage of cells containing nuclear RAD51 foci enumerated & depicted as described in (B). ***p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29606576), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Rad51 Antibody (14B4) [NB100-148] - MiCas9’s beneficial effects are Brex27 & RAD51 dependent.a Indel rates by spCas9, miCas9, & miCas9-mutant at the on-target site for sg-AAVS1 & the off-target site 1 for sg-EMX1. b Indel rates estimated by T7E1 assay by spCas9 & miCas9 with or without the use of RAD51 inhibitor, B02. c Co-IP results by using anti-flag (left) or anti-RAD51 (right) antibodies followed western blot for RAD51 or Cas9. d ChIP assay results with anti-RAD51 antibody at the proximal region of the sg-AAVS1 target locus. Sp: spCas9, Mi: miCas9, Mi-mut: miCas9-mutant. #Reads: Average amplicon reads per sample. Three independent experiments were performed for each condition. Data are presented as mean ± standard error of means (SEM). Unpaired t-test (two tailed) was used to compare data using GraphPad Prism 8 software (GraphPad Software, Inc., San Diego, CA). Source data are available in the Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33247137), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, Func, ICC/IF, IHC, IP, ChIP, KD
• Clone: 14B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rad51 Antibody (14B4) - Azide and BSA Free Summary

• Immunogen: Full length (amino acids 1-338) Rad51 expressed in E. coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD51
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation
  • In vitro assay
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500 - 1:3000
• Application Notes: ChIP usage reported in scientific literature (PMID: 24023853). Rad51 antibody validated for IHC-Fr, ICC/IF from verified customer reviews. Use in In vitro assay reported in scientific literature (PMID: 27815389). PLA-Assay dependent.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Bladder Whole Tissue Lysate (Adult Whole Tumor)	Loading...
  Rad51 Overexpression Lysate	Loading...

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 23942865). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Rad51 Antibody (14B4) - Azide and BSA Free

• BRCC5
• HRAD51
• HsRad51
• HsT16930
• RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
• RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• RAD51 homolog A
• Rad51
• RAD51ABRCA1/BRCA2-containing complex, subunit 5
• RAD51L3
• RecA, E. coli, homolog of
• RECADNA repair protein RAD51 homolog 1
• RecA-like protein
• recombination protein A

Background

Rad51, a 37 kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad51 Antibody (NB100-148)(71)

Publications using NB100-148

• Shell DJ, Foley CA, Wang Q et al. Discovery of a 53BP1 Small Molecule Antagonist Using a Focused DNA-Encoded Library Screen Journal of medicinal chemistry 2023-10-26 [PMID: 37782247] (Human)
• Neizer-Ashun F, Dwivedi SKD, Dey A et al. KRCC1, a modulator of the DNA damage response Nucleic acids research 2022-10-16 [PMID: 36243983] (WB)
• Neizer-Ashun F, Dwivedi S, Dey A et al. KRCC1, a novel modulator of the DNA damage response Research Square 2022-04-08 (WB)
• Fernando P Targeting DNA damage response defects in cancers The University of Queensland 2022-01-21
• Sriramkumar S, Matthews TD, Ghobashi AH, Miller SA Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A is Mediated by Replication Protein A in Ovarian Cancer bioRxiv Jan 1 2020 (WB, Human)
• Moeed, A;Thilmany, N;Beck, F;Puthussery, BK;Ortmann, N;Haimovici, A;Badr, MT;Haghighi, EB;Boerries, M;Öllinger, R;Rad, R;Kirschnek, S;Gentle, IE;Donakonda, S;Petric, PP;Hummel, JF;Pfaffendorf, E;Zanetta, P;Schell, C;Schwemmle, M;Weber, A;Häcker, G; The Caspase-Activated DNase drives inflammation and contributes to defense against viral infection Cell death and differentiation 2024-06-07 [PMID: 38849575]
• Davies B, Zhang G, Moralli D et al. Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice Genome research 2023-11-17 [PMID: 37977820]
  
  Details:
  Sample type: Testis
• Ito M, Furukohri A, Matsuzaki K et al. FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination Nature communications 2023-10-27 [PMID: 37891173] (ChIP, Mouse)
• Pinedo-Carpio E, Dessapt J, Beneyton A et al. FIRRM cooperates with FIGNL1 to promote RAD51 disassembly during DNA repair Science advances 2023-08-09 [PMID: 37556550] (Immunoprecipitation, Human)
• Mauro S, Bolognesi MM, Villa N, Capitoli G et Al. A DNA damage response-like phenotype defines a third of colon cancers at onset FASEB J 2023-06-21 [PMID: 37342943]
• Vugic D, Dumoulin I, Martin C et al. Replication Gap Suppression Depends on the Double-Strand DNA Binding Activity of BRCA2 SSRN Electronic Journal 2022-04-15 [PMID: 36707518]
• Jimenez-Sainz J, Mathew J, Moore G, Lahiri S et Al. BRCA2 BRC missense variants disrupt RAD51-dependent DNA repair Elife 2022-09-13 [PMID: 36098506]
• Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human)
• Zhou Z, Yin H, Suye S et al. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice Reproductive biology 2022-01-10 [PMID: 35026551] (IF/IHC, Mouse)
• McMahon KA, Stroud DA, Gambin Y et al. Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response eLife 2021-06-18 [PMID: 34142659]
• Fernando M, Duijf PHG, Proctor M et al. Dysregulated G2 phase checkpoint recovery pathway reduces DNA repair efficiency and increases chromosomal instability in a wide range of tumours Oncogenesis 2021-05-15 [PMID: 33993200] (WB, ICC/IF, Human)
• Veo BL, Danis E, Pierce A et al. Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in Myc-driven Medulloblastoma Cell Rep 2021-04-28 [PMID: 33910002]
• Kaminski P, Hong S, Kono T, et al. Topoisomerase 2 beta induces DNA breaks to regulate human papillomavirus replication bioRxiv 2021-01-10 [PMID: 33563836]
• Dong C, An L, Yu CH, Huen MSY A DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage Nucleic acids research 2021-01-19 [PMID: 33469661] (ICC/IF)
• Correll-Tash S, Lilley B, Harold Salmons IV et al. Double Strand Breaks (DSBs) as Indicators of Genomic Instability in PATRR-mediated Translocations Human molecular genetics 2020-12-01 [PMID: 33258468]
• Ma, L, Ruan, J Et al. MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Nat Commun 2020-11-27 [PMID: 33247137] (WB, Mouse)
• GarcIa-Muse T Detection of DSBs in C. elegans Meiosis Methods Mol. Biol. 2020-08-26 [PMID: 32840787]
• Sriramkumar, S, Matthews, T D Et al. Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A Is Mediated by Replication Protein A in Ovarian Cancer. Mol Cancer Res 2020-11-01 [PMID: 32801161] (ICC/IF, Human)
• Hinch AG, Becker PW, Li T et al. The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates Mol. Cell 2020-06-23 [PMID: 32610038]
• Kono T, Hoover P, Poropatich K et al. Activation of DNA damage repair factors in HPV positive oropharyngeal cancers Virology 2020-01-01 [PMID: 32560902] (IHC-P, Human)
• Feng W, Simpson DA, Carvajal-Garcia J et al. Genetic determinants of cellular addiction to DNA polymerase theta Nat Commun [PMID: 31537809] (ICC/IF, Mouse)
• Bai Y, Wang W, Li S et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex Mol. Cell 2019-07-09 [PMID: 31353207]
• Tu Z, Mu X, Chen X, et al. Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes Environ. Pollut. 2019-05-24 [PMID: 31158667] (ICC/IF, Mouse)
• Deveryshetty, J;Peterlini, T;Ryzhikov, M;Brahiti, N;Dellaire, G;Masson, JY;Korolev, S; Novel RNA and DNA strand exchange activity of the PALB2 DNA Binding Domain and its critical role for DNA repair in cells Elife 2019-04-24 [PMID: 31017574] (WB, Human)
• Shin N, Cuenca L, Karthikraj R et al. Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans PLoS Genet. 2019-02-01 [PMID: 30763314]
• Garcia-Muse T, Galindo-Diaz U, Garcia-Rubio M et al. A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs. Cell Rep 2019-01-15 [PMID: 30650366]
• Sulkowski PL, Sundaram RK, Oeck S et al. Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair. Nat. Genet. 2018-08-01 [PMID: 30013182] (WB, Human)
• PeriaSamy J, Kurdekar V, Jasti S et al. Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling. Cell Chem Biol 2018-03-09 [PMID: 29606576] (ICC/IF)
• Scanlon SE, Hegan DC, Sulkowski PL, Glazer PM. Suppression of homology-dependent DNA double-strand break repair induces PARP inhibitor sensitivity inVHL-deficient human renal cell carcinoma. Oncotarget. 2018-01-12 [PMID: 29435132] (WB, Human)
• Li Q, Xie W, Wang N et al. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol 2018-04-01 [PMID: 29413763]
• Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017-06-12 [PMID: 28604711] (ICC/IF, Human)
• Nadarajan S, Lambert TJ, Altendorfer E et al. Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop. Elife. 2017-03-27 [PMID: 28346135]
• Pauty J, Couturier AM, Rodrigue A et al. Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif. Nucleic Acids Res. 2017-03-17 [PMID: 28158555]
• Phillips AF, Millet AR, Tigano M et al. Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion Mol. Cell 2017-01-11 [PMID: 28111015]
• Li M, Yu X, Chen Q. Chemopreventive effects of ROS targeting in a murine model of BRCA1-deficient breast cancer. Cancer Res. 2016-11-04 [PMID: 27815389] (In vitro, Human)
• Ackermann L, Schell M, Pokrzywa W et al. E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis Nat. Struct. Mol. Biol. 2016-09-26 [PMID: 27669035] (IF/IHC, C. elegans)
• Dok R, Abbasi Asbagh L, Van Limbergen EJ et al. Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers. Oncotarget 2016-05-26 [PMID: 27246975] (IF/IHC, Human)
• Liu H, Yan P, Fanning E. Human DNA helicase B functions in cellular homologous recombination and stimulates rad51-mediated 5'-3' heteroduplex extension in vitro PLoS ONE. 2015-01-25 [PMID: 25617833] (ICC/IF, Human)
  
  Details:
  RAD52 antibody used for ICC-IF on U2OS cells which were transfected with human DNA helicase B/HDHB. The immunostaining assay involved - treatment of cells with 5 Gy IR radiation, extraction of cells with 0.2% Triton X-100 in CSK buffer, fixation of cells with 3.7 % PFA at RT for 20 minutes, primary antibody incubation at 1:300 dilution in PBS-10%FBS, detection with IF labelled secondary antibody (Figure 3)
• Cornago M, Garcia-Alberich C, Blasco-Angulo N et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014-10-03 [PMID: 25275596] (WB, Human)
  
  Details:
  Survivin antibody used for ICC-IF on U251-MG cells in metaphase after HDAC inhibitor suberanilohydroxamic acid/SAHA treatment (Figure 7a). Antibody also used for WB on lysates of glioma cell lines U87, U251-MG, LN229 cells as well as human GBM primary cultures C55, C65, C48 and C60 treated or not with HDAC inhibitor SAHA and valproic acid/VPA (Figure 7b and c)
• Del Nagro Christopher J, Choi Jonathan, Xiao Yang et al. Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death. Cell Cycle. 2014-01-01 [PMID: 24247149] (ICC/IF, Human)
• Wu X, Xu Y, Feng K et al. MutS Homologue hMSH5: Recombinational DSB Repair and Non-Synonymous Polymorphic Variants. PLoS One. 2013-09-04 [PMID: 24023853] (Chemotaxis, Human)
• Lans H, Lindvall JM, Thijssen K et al. DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals. Cell Death Differ. 2013-09-06 [PMID: 24013725] (ICC/IF, IF/IHC, C. elegans)
• Buonanno M, Garty G, Grad M et al. Microbeam irradiation of C. elegans nematode in microfluidic channels. Radiat Environ Biophys. 2013-08-13 [PMID: 23942865] (IF/IHC, ICC/IF, C. elegans)
• Chu YL, Wu X, Xu Y, Her C. MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair. Mol Cancer 2013-06-02 [PMID: 23725059] (WB, Human)
• Tompkins JD, Wu X, Her C. MutS homologue hMSH5: role in cisplatin-induced DNA damage response. Mol Cancer;11:10. 2012-03-08 [PMID: 22401567] (WB, Human)
• Moldovan G-L, Dejsuphong D, Petalcorin MIR et al. Inhibition of Homologous Recombination by the PCNA-Interacting Protein PARI. Molecular Cell. 2011-12-06 [PMID: 22153967]
• Meerang M, Ritz D, Paliwal S et al. The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks. Nat Cell Biol;13(11):1376-82. 2011-10-23 [PMID: 22020440]
• Harper NC, Rillo R, Jover-Gil S et al. Pairing Centers Recruit a Polo-like Kinase to Orchestrate Meiotic Chromosome Dynamics in C. elegans Developmental Cell. 2011-10-19 [PMID: 22018922]
• Lee M, Daniels MJ, Garnett MJ et al. A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor. Oncogene 2011-07-01 [PMID: 21399666] (ICC/IF)
• Klammer H, Kadhim M, Iliakis G et al. Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells. Cancer Res 2010-11-01 [PMID: 20861183] (ICC/IF)
• Ammazzalorso F, Pirzio LM, Bignami M et al. ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery. EMBO J 2010-09-01 [PMID: 20802463] (ICC/IF)
• Graeser M, McCarthy A, Lord CJ et al. A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer. Clin Cancer Res 2010-12-01 [PMID: 20802015] (ICC/IF)
• Bowen C, Gelmann EP. NkX3.1 activates cellular response to DNA damage. Cancer Res 2010-04-01 [PMID: 20395202] (ICC/IF)
• Suzuki K, Yamauchi M, Oka Y et al. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res 2010-07-01 [PMID: 20385602] (ICC/IF)
• Moldovan GL, Madhavan MV, Mirchandani KD et al. DNA polymerase POLN participates in crosslink repair and homologous recombination. Mol Cell Biol 2009-12-07 [PMID: 19995904]
• Dungey FA, Caldecott KW, Chalmers AJ. Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90. Mol Cancer Ther 2009-08-01 [PMID: 19671736] (ICC/IF)
• Carlessi L, De Filippis L, Lecis D et al. DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression. Cell Death Differ 2009-06-01 [PMID: 19229246] (ICC/IF)
• Stewart GS, Stankovic T, Byrd PJ et al. RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Proc Natl Acad Sci U S A 2007-10-01 [PMID: 17940005]
• Shim, K et al. hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51. J Biol Chem;279(29):30385-94. 2004-07-16 [PMID: 15123651]
• Warren M, Lord CJ, Masabanda J et al. Phenotypic effects of heterozygosity for a BRCA2 mutation. Hum Mol Genet 2003-10-01 [PMID: 12928478] (ICC/IF)
• Kao GD, McKenna WG, Guenther MG et al. Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response. J Cell Biol 2003-03-01 [PMID: 12668657] (ICC/IF)
• Tarsounas, N et al. BRCA2-dependent independent formation of RAD51 nuclear foci. Oncogene. 22:1115-1123. 2003-01-01 [PMID: 12606939]
• French, CA et al. Role of mammalian RAD51L2 (RAD51C) in recombination genetic stability. J Biol Chem 277:19322-19330. 2002-01-01 [PMID: 11912211]
• Wiese C, Collins DW, Albala JS et al. Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells. Nucleic Acids Res 2002-02-15 [PMID: 11842112]
• Masson, J et al. Identification purification of two distinct complexes containing the five Rad51 paralogs. Genes Dev 15:3296-3207. 2001-01-01 [PMID: 11751635]
• Masson, J et al. The meiosis-specific recombinase hDms1 forms ring structures interacts with hRad51. EMBO J. 18:6552-6560. 1999-01-01 [PMID: 10562567]

Reviews for Rad51 Antibody (NB100-148) (5)

Average Rating: 4.8

(Based on 5 reviews)

We have 5 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Marina Rodriguez Candela ICC Human 06/14/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: human immortalized breast cancer cells
• Species: Human
• Lot: 43574

Comments

• Comments: Cells were fixed with 4% PFA. RAD51 Ig was diluted 1:200.

reviewed by: Verified Customer ICC Mouse 10/09/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: testis cells
• Species: Mouse
• Lot: 42590

reviewed by: Verified Customer IHC-Fr Other 12/12/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23942865
• Species: Other

reviewed by: Manuela Buonanno IHC-Fr Other 11/07/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: whole C. elegans nematode
• Species: Other

reviewed by: Western Forte WB Human 06/20/2012

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: RAD51
• Entrez:
  • 5888(Human)
• Uniprot:
  • Q06609()