![Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Western blot experiment using Rad52 antibody to detect human Rad52 protein expression in HeLa whole cell lysate .](http://images.novusbio.com/fullsize/RAD52-Antibody-5H9-Western-Blot-NBP1-41208-img0001.jpg "Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Western blot experiment using Rad52 antibody to detect human Rad52 protein expression in HeLa whole cell lysate .")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | 5H9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Human RAD52 recombinant protein expressed in E. coli. |
| Specificity | This antibody will recognize human Rad52. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAD52 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RAD52 antibody [5H9] (NBP1-41208) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-41208_mouse-monoclonal-rad52-antibody-5h9-91020242075956.jpg "Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RAD52 antibody [5H9] (NBP1-41208) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")