Rad9 Antibody (93A535) - Azide and BSA Free

Western Blot: Rad9 Antibody (93A535) - Azide and BSA Free [NBP2-80924] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test. Image from the standard format of this antibody.

Immunohistochemistry: Rad9 Antibody (93A535) - Azide and BSA Free [NBP2-80924] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 93A535
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Rad9 Antibody (93A535) - Azide and BSA Free Summary

• Immunogen: The antibody was raised against fusion protein of human full length Rad9.
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD9A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (Kotmatsu et al)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Lee et al (2007))
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Rad9 Antibody (93A535) - Azide and BSA Free

• cell cycle checkpoint control protein RAD9A
• DNA repair exonuclease rad9 homolog A
• EC 3.1.11.2
• hRAD9
• RAD9 (S. pombe) homolog
• RAD9 homolog A (S. pombe)
• RAD9

Background

Rad9 is a recently isolated human protein that is involved in the control of cell cycle checkpoint. Human Rad9 (hRad9) is homologue of the S. pombe Rad9. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage check point response in S. pombe. Recently, it has been shown that hRad9 interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Over-expression of hRad9 in mammalian cells leads to apoptosis, where as antisense Rad9 suppresses cell death induced by DNA damaging agents such as methanosulphonate. Human Rad9 undergoes complex post-translational modifications in undamaged cells and is hyperphosphorylated in response to DNA damage and makes ternary complex with human Rad1 and human HUS1 proteins. This may account for detection of multiple protein bands in some cases by western blot or immunoprecipitation analysis using anti-hRad9 antibody. HRad9 has an intrinsic 3-5 exonuclease activity suggesting that by interaction with DNA it may play a role in the damage-sensing step of the DNA damage checkpoint pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD9A