Raf-1 Antibody (1H4) - Azide and BSA Free

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi or non-transfected control. Blot probed with H00005894-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in HeLa.

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in rat brain.

Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between BAD and RAF1. HeLa cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-RAF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction compl

Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-RAF1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Func, KD
• Clone: 1H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Raf-1 Antibody (1H4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAF1 (AAH18119, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDF
• Specificity: RAF1 - v-raf-1 murine leukemia viral oncogene homolog 1 (1H4)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAF1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Raf-1 Antibody (1H4) - Azide and BSA Free

• cRaf
• c-Raf
• c-Raf-1
• CRAFraf proto-oncogene serine/threonine protein kinase
• EC 2.7.11
• EC 2.7.11.1
• NS5
• Proto-oncogene c-RAF
• RAF proto-oncogene serine/threonine-protein kinase
• RAF
• Raf1
• Raf-1
• Raf-1Oncogene RAF1
• v-raf-1 murine leukemia viral oncogene homolog 1
• v-Raf-1

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAF1
• Entrez:
  • 5894(Human)
• Uniprot:
  • AAH18119(Human)