RalA/RalB Antibody (3E6) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RalA/RalB Antibody (3E6) [H00005898-M01] - Analysis of monoclonal antibody to RALA on HeLa cell . Antibody concentration 10 ug/ml.

Proximity Ligation Assay: RalA/RalB Antibody (3E6) [H00005898-M01] - Analysis of protein-protein interactions between RALBP1 and RALA. HeLa cells were stained with anti-RALBP1 rabbit purified polyclonal 1:1200 and anti-RALA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: ELISA, Func, ICC/IF
• Clone: 3E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RalA/RalB Antibody (3E6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein. Mouse monoclonal antibody raised against a partial-length synthetic RALA.
• Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S194 of human RALA. The mark "*" on the immunogen sequence is the site of phosphorylation. CNGKKKRKS*LAKRI
• Specificity: RALA - v-ral simian leukemia viral oncogene homolog A (ras related) (3E6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RALA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
• Application Notes: It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RalA/RalB Antibody (3E6) - Azide and BSA Free

• MGC48949
• RAL
• RalA/RalB
• Ras family small GTP binding protein RALA
• RAS-like protein A
• ras-related protein Ral-A
• v-ral simian leukemia viral oncogene homolog A (ras related)

Background

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RalA/RalB Antibody (H00005898-M01)(1)

Publication using H00005898-M01

• Capdevila C, Miller J, Cheng L, Kornberg A et Al. Time-resolved fate mapping identifies the intestinal upper crypt zone as an origin of Lgr5+ crypt base columnar cells Cell 2024-06-07 [PMID: 38848677]

Bioinformatics

• Gene Symbol: RALA
• Entrez:
  • 5898(Human)
• Uniprot:
  • NP_005393(Human)