RAP1A Antibody (RAP1 4C8/1) - BSA Free

Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: RAP1 4C8/1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.43 mg/ml

RAP1A Antibody (RAP1 4C8/1) - BSA Free Summary

• Immunogen: Human RAP1A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAP1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): HeLa cell and mouse testis extracts. This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.43 mg/ml
• Purity: Protein A purified

Alternate Names for RAP1A Antibody (RAP1 4C8/1) - BSA Free

• C21KG
• G-22K
• GTP-binding protein smg p21A
• KREV-1
• KREV1Ras-related protein Krev-1
• RAP1
• Rap1A
• RAP1A, member of RAS oncogene family
• RAS-related protein RAP1A
• ras-related protein Rap-1A
• SMGP21

Background

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAP1A Antibody (NBP2-50269)(2)

Publications using NBP2-50269

• Wang S, Wang D, Chang Y et al. Elevated RAP1A expression correlates with the severity of acute GVHD after umbilical cord blood transplantation Transplant Immunology 2022-01-01 [PMID: 35114361] (FLOW, Human)
• Shibayama Y, Kubo Y, Nakagawa T, Iseki K MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A Biol. Pharm. Bull. 2019-08-02 [PMID: 31366868] (WB, Human)

Bioinformatics

• Gene Symbol: RAP1A
• Uniprot:
  • A2ALS5(Mouse)