RBM15B Antibody (A10D12+A10D11)

Western Blot: RBM15B Antibody (A10D12+A10D11) [NBP3-32898] - Western blot analysis of RBM15B on different lysates with Mouse anti-RBM15B antibody (NBP3-32898) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27602518). The bands below 100 kDa may be caused by degradation. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32898) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: A10D12+A10D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RBM15B Antibody (A10D12+A10D11) Summary

• Immunogen: Recombinant protein within human RBM15B aa 1-200 / 890. (Uniprot: Q8NDT2)
• Localization: Nucleus, nucleoplasm, Nucleus speckle, Nucleus envelope.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBM15B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RBM15B Antibody (A10D12+A10D11)

• chromosome 3p21.1 gene sequence
• HUMAGCGB
• one-twenty two protein 3
• RNA binding motif protein 15B
• RNA-binding motif protein 15B

Background

Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 (PubMed 16129689)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM15B