Recombinant Human BDNF Protein, Animal-Free Summary
Details of Functionality |
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED 50 for this effect is 0.100-1.00 ng/mL. The specific activity of Recombinant Human BDNF is >8.0 x
105 units/mg, which is calibrated against the human BDNF WHO
Standard (NIBSC code: 96/534). |
Source |
E. coli-derived human BDNF protein Arg134-Arg247, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. |
Accession # |
|
N-terminal Sequence |
Met |
Protein/Peptide Type |
Animal-Free Recombinant Proteins |
Purity |
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
13.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
12 kDa, under reducing conditions. |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Purity |
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute the 25 μg size at 250 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human BDNF Protein, Animal-Free
Background
Brain-derived
neurotrophic factor (BDNF) is a member of the neurotrophin family of growth
factors that are required for the differentiation and survival of specific
neuronal subpopulations in both the central as well as the peripheral nervous
system.
BDNF
is often utilized as a cell culture supplement to promote neural
differentiation in regenerative medicine studies and clinical manufacturing
protocols.
The
neurotrophin family is comprised of at least four proteins including BDNF,
nerve growth factor (NGF), neurotrophin-3 (NT3), and NT4/5. Human BDNF is
initially expressed as a proprotein, which is then cleaved to yield a mature
protein. Mature BDNF is a non-covalently linked homodimer, with each monomer
containing antiparallel beta -strands and a characteristic cystine knot motif.
Within the mature domain, human BDNF shares the identical amino acid sequence
with mature mouse and rat BDNF.
BDNF
is strongly expressed in various regions of the brain, including the
hippocampus and cerebellum, and weaker expression has been detected in the
thymus, liver, spleen, heart, and lung. BDNF participates in axonal growth and
pathfinding and in the modulation of dendritic growth and morphology and in
later stages of development regulates synaptic transmission and plasticity and
acts as a central modulator of pain. BDNF binds with high affinity and
specifically activates the TrkB tyrosine kinase receptor. BDNF signaling via
TrkB is essential for adult synaptic plasticity and the formation of memories.
The
BDNF signaling pathway utilizes both AKT and ERK pathways to exert its pleiotrophic
effects in the central nervous system. Decreased expression of BDNF is seen in
many neurological diseases such as Alzheimer's disease, Parkinson's disease,
Huntington's disease, and autism. Further, BDNF is proposed as a biomarker for
psychiatric disorders such as bipolar disease and depression and has been
implicated in posttraumatic stress disorder, phobia, and panic disorder. A
single amino acid substitution, Val66Met, has been shown to lead to reduced,
activity-dependent BDNF secretion and memory impairment.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB
Species: Hu
Applications: BA
Species: Rt
Applications: IHC, WB
Species: Hu
Applications: BA
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: Bind, BA
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ICC, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
Species: Hu
Applications: BA
Publications for BDNF (BT-BDNF-AFL) (0)
There are no publications for BDNF (BT-BDNF-AFL).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for BDNF (BT-BDNF-AFL) (0)
There are no reviews for BDNF (BT-BDNF-AFL).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for BDNF (BT-BDNF-AFL) (0)