Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

Recombinant human EPO (Catalog # 287-TC) has a molecular weight (MW) of 28.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Recombinant Human Erythropoietin / EPO (287-TC) stimulates cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner. The ED50 for this effect is 0.015-0.075 units/mL.

Recombinant Human Erythropoietin R Fc Chimera (963-ER) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human Erythropoietin/EPO (287-TC) was measured at a concentration range between 0.488 pM and 1000 pM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.219 pM.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.015-0.075 units/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein
  Ala28-Arg193
• Accession #: NP_000790.2
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPO
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt at -20 to -70 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Reconstitution Instructions: Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of the EPO protein is required for biological activities in vivo (4). The mature human EPO protein shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

1. Koury, M.J. (2005) Exp. Hematol. 33:1263.
2. Jacobs, K. et al. (1985) Nature 313:806.
3. Wen, D. et al. (1993) Blood 82:1507.
4. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
6. Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
7. Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
10. Acs, G. et al. (2001) Cancer Res. 61:3561.
11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

Publications for Erythropoietin/EPO (287-TC)(77)

Publications using 287-TC

• Zhang, H;Zhang, Q;Chen, X;Wang, M;Li, L;Yu, C; Establishment of a transgene-free iPS cell line (SDCHi008-A) from a young patient bearing a KCNQ2 mutation and suffering from Epilepsy Stem cell research 2024-07-24 [PMID: 39079397] (Bioassay, Human)
• Zakaria, MF;Sonoda, S;Kato, H;Ma, L;Uehara, N;Kyumoto-Nakamura, Y;Sharifa, MM;Yu, L;Dai, L;Yamauchi-Tomoda, E;Aijima, R;Yamaza, H;Nishimura, F;Yamaza, T; Erythropoietin receptor signal is crucial for periodontal ligament stem cell-based tissue reconstruction in periodontal disease Scientific reports 2024-03-20 [PMID: 38509204] (Bioassay, Human)
• Li, Z;Liu, C;Xin, H;Lv, Y;Gao, M;Ma, J;Liu, N;Gai, Z;Liu, Y; Establishment of a non-integrated iPSC line (SDQLCHi043-A) from a male infant with propionic acidemia carrying compound heterozygote mutations in PCCB gene Stem cell research 2024-02-17 [PMID: 38394970] (Bioassay, Human)
• Yang, X;Fan, Y;Li, Y;Zhang, H;Wang, B;Guan, J;Gao, J;Ma, X;Liu, Y; Generation and characterization of PBMCs-derived human induced pluripotent stem cell (iPSC) line SDQLCHi050-A from a healthy donor Stem cell research 2023-12-10 [PMID: 38128452] (Bioassay, Human)
• Gao, C;Liu, Y;Liu, N;Li, Z;Yang, X;Yang, Y;Xi, Y;Tian, J;Gai, Z; Establishment of human induced pluripotent stem cell line (SDQLCHi060-A) from a patient with AUTS2 syndrome carrying a heterozygous mutation in AUTS2 gene Stem cell research 2023-11-03 [PMID: 37948839] (Bioassay, Human)
• Wang, Y;Yang, X;Zhang, H;Liu, N;Liu, Y;Gai, Z;Liu, Y;Lv, Y; Generation of a transgene-free iPS cell line (SDQLCHi053-A) from a young girl carrying a heterozygous mutation (c.427C &gt; T) in SYNGAP1 gene Stem cell research 2023-06-07 [PMID: 37385133] (Bioassay, Human)
• Wan, Z;Guan, J;Li, Y;Zhang, H;Wang, B;Yang, X;Jin, X;Ma, X;Liu, Y; Generation and characterization of a human induced pluripotent stem cell line (SDQLCHi052-A) from peripheral blood mononuclear cells derived from a healthy Chinese donor Stem cell research 2023-06-11 [PMID: 37320988] (Bioassay, Human)
• Fan, Y;Li, Y;Yang, X;Zhang, H;Wang, B;Guan, J;Gao, J;Ma, X;Liu, Y; Generation and characterization of PBMCs-derived human induced pluripotent stem cell (iPSC) line SDQLCHi051-A from an autism spectrum disorder patient with compound CHD8 gene mutations Stem cell research 2023-06-01 [PMID: 37229974] (Bioassay, Human)
• D Ai, X Yang, X Li, N Liu, X Xie, Y Liu, W Song Generation of an induced pluripotent stem cell line SDPHi002-A from a patient with lung cancer Stem Cell Research, 2023-04-15;69(0):103096. 2023-04-15 [PMID: 37104933] (Bioassay, Human)
• Y Xi, H Zhang, B Wang, W Song, Y Liu Establishment of an induced pluripotent stem cell line SDQLCHi049-A from a healthy male neonate Stem Cell Research, 2023-04-15;69(0):103097. 2023-04-15 [PMID: 37075622] (Bioassay, Human)
• Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen Mapping of clonal lineages across developmental stages in human neural differentiation Cell Stem Cell, 2023-03-17;0(0):. 2023-03-17 [PMID: 36933556] (Bioassay, Human)
• Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen Mapping of clonal lineages across developmental stages in human neural differentiation Cell Stem Cell, 2023;0(0):. 2023 [PMID: 36933556] (Bioassay, Human)
• X Wang, S Cooper, HE Broxmeyer, R Kapur Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment Leukemia, 2022-12-02;0(0):. 2022-12-02 [PMID: 36460765] (Cell Culture, Human)
• PY Su, W Lee, J Zernant, SH Tsang, T Nagasaki, B Corneo, R Allikmets Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture Oncogene, 2022-11-14;65(0):102973. 2022-11-14 [PMID: 36455383] (Bioassay, Human)
• Y Wang, Y Lv, X Yang, Y Li, Z Li, Z Gao, Z Gai, Y Liu Generation of an induced pluripotent stem cell line (SDQLCHi044-A) from a patient with autosomal dominant mental retardation type 5 harboring heterozygous mutation in SYNGAP1 gene Stem Cell Research, 2022-09-17;64(0):102922. 2022-09-17 [PMID: 36183676] (Bioassay, Human)
• YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022-08-24;8(34):eabn5522. 2022-08-24 [PMID: 36001668] (Bioassay, Human)
• A Ramshekar, CA Bretz, E Kunz, T Cung, BT Richards, GJ Stoddard, GS Hageman, B Chaqour, ME Hartnett Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization Biomedicines, 2022-07-09;10(7):. 2022-07-09 [PMID: 35884958] (Bioassay, Human)
• SA Luff, JP Creamer, S Valsoni, C Dege, R Scarfò, A Dacunto, S Cascione, LN Randolph, E Cavalca, I Merelli, SA Morris, A Ditadi, CM Sturgeon Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells Nature Cell Biology, 2022-04-28;0(0):. 2022-04-28 [PMID: 35484246] (Bioassay, Human)
• G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL Stem Cell Research, 2021-12-23;59(0):102635. 2021-12-23 [PMID: 35074682] (Bioassay, Human)
• AF de Souza, FF Bressan, NCG Pieri, RC Botigelli, T Revay, SK Haddad, DT Covas, ES Ramos, WA King, FV Meirelles Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients Cells, 2021-11-10;10(11):. 2021-11-10 [PMID: 34831322] (Bioassay, Human)
• ML Wegscheid, C Anastasaki, KA Hartigan, OM Cobb, JB Papke, JN Traber, SM Morris, DH Gutmann Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis Cell Reports, 2021-07-06;36(1):109315. 2021-07-06 [PMID: 34233200] (Bioassay, Human)
• G Iannello, A Patel, D Sirabella, B Corneo, V Thaker Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Stem Cell Research, 2021-06-21;54(0):102432. 2021-06-21 [PMID: 34214899] (Bioassay, Human)
• Z Li, X Zhang, X Li, Y Yang, H Xin, X Yang, N Liu, Z Gai, Y Liu A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene Stem Cell Research, 2021-03-26;53(0):102313. 2021-03-26 [PMID: 34087978] (Cell Culture, Human)
• J Borsoi, M Morato-Mar, F de Araújo, L Assis Pere, LE Farinha-Ar, R Delgado Sa, AB Alvarez Pe, L Veiga Pere Generation of two human induced pluripotent stem cell (hiPSC) lines derived from unrelated Marfan Syndrome patients Stem Cell Research, 2021-05-25;54(0):102407. 2021-05-25 [PMID: 34062330] (Bioassay, Human)
• H Zhang, C Liu, Y Ma, L Lin, Y Lv, M Gao, Z Gai, Y Liu Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene Stem Cell Research, 2021-04-09;53(0):102331. 2021-04-09 [PMID: 33882394] (Bioassay, Human)
• X Yang, N Liu, H Mu, Y Lv, H Zhang, Y Li, J Guan, Z Gai, Y Liu Reprogramming of human peripheral blood mononuclear cell (PBMC) from a patient suffering from hearing loss into iPSC line (SDQLCHi035-A) maintaining compound heterozygous variations in GJB2 gene Stem Cell Research, 2021-01-21;51(0):102188. 2021-01-21 [PMID: 33517119] (Bioassay, Human)
• X Yang, C Duan, H Zhang, Y Li, J Guan, D Wang, Y Lv, Z Gai, Y Liu Establishment of a non-integrate iPS cell line (SDQLCHi023-A) from a patient with Xq25 microduplication syndrome carrying a 1.3�Mb hemizygote duplication at chrXq25 Stem Cell Research, 2020-12-28;51(0):102147. 2020-12-28 [PMID: 33493992] (Bioassay, Human)
• B Wang, L Yang, Y Li, M Gao, H Zhang, X Yang, J Guan, Y Liu, Z Gai Establishment of a human induced pluripotent stem cell line (SDQLCHi037-A) from a patient with Alagille syndrome carrying heterozygous mutation in JAG1 gene Stem Cell Research, 2021-01-08;51(0):102162. 2021-01-08 [PMID: 33465531] (Bioassay, Human)
• H Zhang, H Zhang, Y Ma, Y Lv, Z Gai, Y Liu Generation and characterization of an induced pluripotent stem cell line SDQLCHi018-A from a congenital myasthenic syndrome patient carrying compound heterozygote mutations in RAPSN gene Stem Cell Research, 2021-01-08;51(0):102160. 2021-01-08 [PMID: 33465529] (Bioassay, Human)
• N Liu, X Yang, S Wang, R Dong, Y Li, Y Lv, Y Liu, Z Gai PBMC-derived integration-free iPSCs line SDQLCHi039-A from a patient with X-linked agammaglobulinemia carrying a novel 9-bp in-frame deletion in BTK gene Stem Cell Research, 2021-01-08;51(0):102165. 2021-01-08 [PMID: 33453577] (Bioassay, Human)
• B Wang, C Liu, H Zhang, Z Gai, Y Liu An induced pluripotent stem cell line (SDQLCHi033-A) derived from a patient with maple syrup urine disease type Ib carrying a homozygous mutation in BCKDHB gene Stem Cell Research, 2020-12-28;50(0):102146. 2020-12-28 [PMID: 33388706] (Bioassay, Human)
• S Riccetti, A Sinigaglia, G Desole, N Nowotny, M Trevisan, L Barzon Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells Viruses, 2020-08-12;12(8):. 2020-08-12 [PMID: 32806715] (Bioassay, Human)
• H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria Int J Mol Sci, 2020-06-15;21(12):. 2020-06-15 [PMID: 32549211] (Bioassay, Human)
• C Brendel, O Negre, M Rothe, S Guda, G Parsons, C Harris, M McGuinness, D Abriss, A Tsytsykova, D Klatt, M Bentler, D Pellin, L Christians, A Schambach, J Manis, H Trebeden-N, M Bonner, E Esrick, G Veres, M Armant, DA Williams Preclinical Evaluation of a Novel Lentiviral Vector Driving Lineage-Specific BCL11A Knockdown for Sickle Cell Gene Therapy Mol Ther Methods Clin Dev, 2020-03-17;17(0):589-600. 2020-03-17 [PMID: 32300607] (Bioassay, Human)
• X Liu, X Yang, Y Li, X Wang, J Ma, W Jiang, Y Liu, W Sun, Y Gong Generation of patient-specific pluripotent induced stem cell line SDUBMSI002-A from a patient with X-linked mental retardation syndrome Stem Cell Res, 2020-02-05;43(0):101724. 2020-02-05 [PMID: 32078988] (Bioassay, Human)
• Y Ma, H Zhang, X Li, X Yang, Y Li, J Guan, Y Lv, Z Gai, Y Liu An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene Stem Cell Res, 2020-02-04;43(0):101729. 2020-02-04 [PMID: 32062132] (Cell Culture, Human)
• J Guan, Z Li, H Zhang, X Yang, Y Ma, Y Li, R Dong, Z Gai, Y Liu Generation of a Human iPSC line (SDQLCHi021-A) from a patient with methylmalonic acidemia cblC type carrying compound heterozygous mutations in MMAHC gene Stem Cell Res, 2020-01-16;43(0):101709. 2020-01-16 [PMID: 32058304] (Cell Culture, Human)
• W Xiaojing, M Yanyan, D Ruonan, L Xiaolin, Z Haiyan, M Jian, L Yi, S Wenjie, L Qiji Generation of a human induced pluripotent stem cell line (SDUBMSi001-A) from a hereditary spastic paraplegia patient carrying kif1a c.773C&gt;T missense mutation Stem Cell Res, 2020-02-04;43(0):101727. 2020-02-04 [PMID: 32045731] (Cell Culture, Human)
• JJ Montoya, MA Turnidge, DH Wai, AR Patel, DW Lee, V Gokhale, LH Hurley, RJ Arceci, C Wetmore, DO Azorsa In vitro activity of a G-quadruplex-stabilizing small molecule that synergizes with Navitoclax to induce cytotoxicity in acute myeloid leukemia cells BMC Cancer, 2019-12-27;19(1):1251. 2019-12-27 [PMID: 31881855] (Bioassay, Human)
• J Guan, X Liu, H Zhang, X Yang, Y Ma, Y Li, Z Gai, Y Liu Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49-50 exons in the DMD gene Stem Cell Res, 2019-11-23;42(0):101666. 2019-11-23 [PMID: 31812072] (Cell Culture, Human)
• H Zhang, Y Ma, X Li, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu An integration-free iPSC line (SDQLCHi013-A) derived from a patient with maple syrup urine disease carrying compound heterozygote mutations in BCKDHA gene Stem Cell Res, 2019-10-12;41(0):101585. 2019-10-12 [PMID: 31775088] (Cell Culture, Human)
• H Zhang, Y Ma, S Yu, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene Stem Cell Res, 2019-10-21;41(0):101611. 2019-10-21 [PMID: 31707209] (Cell Culture, Human)
• J Guan, X Liu, H Zhang, Y Lv, X Wang, X Yang, Y Ma, Q Liu, Y Liu, W Sun Generation of an iPSC line (SDQLCHi015-A) from peripheral blood mononuclear cells of a patient with mental retardation type 15 carrying c.1007_1011del, p.(Ile336fs) in CUL4B gene Stem Cell Res, 2019-10-18;41(0):101628. 2019-10-18 [PMID: 31678776] (Cell Culture, Human)
• Y Ma, H Zhang, S Zhang, R Dong, X Yang, Y Li, J Guan, Z Gai, Y Liu An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene Stem Cell Res, 2019-09-13;41(0):101577. 2019-09-13 [PMID: 31648101] (Cell Culture, Human)
• Y Li, H Zhang, B Yan, Y Ma, X Yang, J Guan, Y Lv, M Gao, J Ma, Z Gai, Y Liu An induced pluripotent stem cell line (SDQLCHi006-A) derived from a patient with maple syrup urine disease type Ib carrying compound heterozygous mutations of p.R168C and p.T322I in BCKDHB gene Stem Cell Res, 2019-09-14;40(0):101579. 2019-09-14 [PMID: 31610500] (Cell Culture, Human)
• Y Ma, H Zhang, X Yang, Y Li, J Guan, Y Lv, H Li, Y Liu, Z Gai Establishment of a human induced pluripotent stem cell line (SDQLCHi004-A) from a patient with nemaline myopathy-4 disease carrying heterozygous mutation in TPM2 gene Stem Cell Res, 2019-09-10;40(0):101559. 2019-09-10 [PMID: 31526942] (Bioassay, Human)
• FS Kittur, Y Lin, E Arthur, CY Hung, PA Li, DC Sane, J Xie Recombinant asialoerythropoetin protects HL-1 cardiomyocytes from injury via suppression of Mst1 activation Biochem Biophys Rep, 2019-01-09;17(0):157-168. 2019-01-09 [PMID: 30671548] (Bioassay, Mouse)
• Park S, Mostoslavsky G Generation of Human Induced Pluripotent Stem Cells Using a Defined, Feeder-Free Reprogramming System. Curr Protoc Stem Cell Biol, 2018-05-04;45(1):e48. 2018-05-04 [PMID: 30040234] (Bioassay, Human)
• L Cyganek, M Tiburcy, K Sekeres, K Gerstenber, H Bohnenberg, C Lenz, S Henze, M Stauske, G Salinas, WH Zimmermann, G Hasenfuss, K Guan Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes JCI Insight, 2018-06-21;3(12):. 2018-06-21 [PMID: 29925689] (Bioassay, Human)
• MC Allenby, A Tahlawi, JCF Morais, K Li, N Panoskalts, A Mantalaris Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids Stem Cells Int, 2018-04-02;2018(0):6230214. 2018-04-02 [PMID: 29760729] (Bioassay, Human)
• R Nagase, D Inoue, A Pastore, T Fujino, HA Hou, N Yamasaki, S Goyama, M Saika, A Kanai, Y Sera, S Horikawa, Y Ota, S Asada, Y Hayashi, KC Kawabata, R Takeda, HF Tien, H Honda, O Abdel-Waha, T Kitamura Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation J. Exp. Med., 2018-04-11;0(0):. 2018-04-11 [PMID: 29643185] (Bioassay, Mouse)
• SY Han, EM Lee, HS Choi, BH Chun, EJ Baek The effects of plasma gelsolin on human erythroblast maturation for erythrocyte production Stem Cell Res, 2018-03-05;29(0):64-75. 2018-03-05 [PMID: 29597129] (Bioassay, Human)
• CM Ferrer, M Alders, AV Postma, S Park, MA Klein, M Cetinbas, E Pajkrt, A Glas, S van Koning, VM Christoffe, MMAM Mannens, L Knegt, JP Etchegaray, RI Sadreyev, JM Denu, G Mostoslavs, MC van Maarle, R Mostoslavs An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality Genes Dev., 2018-03-19;32(5):373-388. 2018-03-19 [PMID: 29555651] (Bioassay, Human)
• HJ Yoon, X Zhang, MG Kang, GJ Kim, SY Shin, SH Baek, BN Lee, SJ Hong, JT Kim, K Hong, H Bae Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion Int J Mol Sci, 2018-01-17;19(1):. 2018-01-17 [PMID: 29342111] (Bioassay, Human)
• S Miao, SM Wang, X Cheng, YF Li, QS Zhang, G Li, SQ He, XP Chen, P Wu Erythropoietin promoted the proliferation of hepatocellular carcinoma through hypoxia induced translocation of its specific receptor Cancer Cell Int., 2017-12-11;17(0):119. 2017-12-11 [PMID: 29238266] (Bioassay, Human)
• N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence Nat Commun, 2017-05-25;8(0):15380. 2017-05-25 [PMID: 28541275] (Bioassay, Human)
• E Arthur, FS Kittur, Y Lin, CY Hung, DC Sane, J Xie Plant-Produced Asialo-Erythropoietin Restores Pancreatic Beta-Cell Function by Suppressing Mammalian Sterile-20-like Kinase (MST1) and Caspase-3 Activation Front Pharmacol, 2017-04-19;8(0):208. 2017-04-19 [PMID: 28469576] (Bioassay, Rat)
• Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system Gene Ther., 2016-06-30;23(10):699-707. 2016-06-30 [PMID: 27356951] (ELISA (Control), Human)
• Canli O, Alankus Y, Grootjans S, Vegi N, Hultner L, Hoppe P, Schroeder T, Vandenabeele P, Bornkamm G, Greten F Glutathione peroxidase 4 prevents necroptosis in mouse erythroid precursors. Blood, 2015-10-13;127(1):139-48. 2015-10-13 [PMID: 26463424] (Bioassay, Mouse)
• Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, Gao Y, Mendelsohn L, Cheng L Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation. Stem Cells, 2015-05-01;33(5):1470-9. 2015-05-01 [PMID: 25702619] (Bioassay, Human)
• Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor. Stem Cells, 2015-03-01;33(3):976-87. 2015-03-01 [PMID: 25446279] (Bioassay, Mouse)
• Kernytsky A, Wang F, Hansen E, Schalm S, Straley K, Gliser C, Yang H, Travins J, Murray S, Dorsch M, Agresta S, Schenkein D, Biller S, Su S, Liu W, Yen K IDH2 mutation-induced histone and DNA hypermethylation is progressively reversed by small-molecule inhibition. Blood, 2014-11-14;125(2):296-303. 2014-11-14 [PMID: 25398940] (Bioassay, Human)
• Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood, 2014-07-02;124(9):1393-403. 2014-07-02 [PMID: 24990886] (Bioassay, Mouse)
• Bu Y, Su F, Wang X, Gao H, Lei L, Chang N, Wu Q, Hu K, Zhu X, Chang Z, Meng K, Xiong J Protein tyrosine phosphatase PTPN9 regulates erythroid cell development through STAT3 dephosphorylation in zebrafish. J Cell Sci, 2014-04-11;127(0):2761-70. 2014-04-11 [PMID: 24727614] (Bioassay, Human)
• Vannucchi , A M, Rotunno , G, Bartalucci , N, Raugei , G, Carrai , V, Balliu , M, Mannarelli , C, Pacilli , A, Calabresi , L, Fjerza , R, Pieri , L, Bosi , A, Manfredini , R, Guglielmelli , P Calreticulin mutation-specific immunostaining in myeloproliferative neoplasms: pathogenetic insight and diagnostic value. Leukemia, 2014-03-12;28(9):1811-8. 2014-03-12 [PMID: 24618731] (Bioassay, Human)
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• Huang , Xiaosong, Shah , Siddhart, Wang , Jing, Ye , Zhaohui, Dowey , Sarah N, Tsang , Kit Man, Mendelsohn , Laurel G, Kato , Gregory, Kickler , Thomas S, Cheng , Linzhao Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors. Mol Ther, 2013-09-03;22(2):451-63. 2013-09-03 [PMID: 24002691] (Bioassay, Human)
• Ma L, Clayton J, Walgren R, Zhao B, Evans R, Smith M, Heinz-Taheny K, Kreklau E, Bloem L, Pitou C, Shen W, Strelow J, Halstead C, Rempala M, Parthasarathy S, Gillig J, Heinz L, Pei H, Wang Y, Stancato L, Dowless M, Iversen P, Burkholder T Discovery and characterization of LY2784544, a small-molecule tyrosine kinase inhibitor of JAK2V617F. Blood Cancer J, 2013-04-12;3(0):e109. 2013-04-12 [PMID: 23584399] (Bioassay, Human)
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Bioinformatics

• Gene Symbol: EPO
• Uniprot:
  • NP_000790.2(Human)