Recombinant Human Integrin alpha 4 beta 7 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Integrin alpha 4 beta 7 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 10⁴ cells per well are added to Recombinant Human Integrin alpha 4/ beta 7/LPAM-1 coated plates (10 μg/mL, 100 μL/well), approximately
  50-80% will adhere after 60 minutes at 37 °C.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 4 beta 7/LPAM-1 protein
  

  Human Integrin alpha 4 (Tyr34-Gln970 (R591L & R878Q)) Accession # P13612	Acidic tail	HHHHHH
  Human Integrin beta 7 (Glu20-His723) Accession # P26010	Basic tail
  N-terminus		C-terminus

• Accession #: P13612 (Integrin a4); P26010 (Integrin beta 7)
• N-terminal Sequence: Tyr34 (Integrin alpha 4) & Glu20 (Integrin beta 7)
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGA4
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 112.8 kDa (Integrin alpha 4) & 84.7 kDa (Integrin beta 7).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-155 kDa and 100-130 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and MgCl₂.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 4 beta 7 Protein, CF

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49d/beta7
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4 beta 7
• integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• LPAM1
• LPAM-1
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha

Background

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 (1). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 150 kDa alpha 4 (CD49d) subunit and a 130 kDa beta 7 subunit (2, 3). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure followed by thigh, calf-1, and calf-2 domains (1). The beta 7 ECD contains a vWFA domain, which interacts with the
alpha 4 beta -propeller to form a binding domain. Metal ion binding sites termed MIDAS and LIMBS promote firm adhesion, and another site termed ADMIDAS is a negative regulatory site that promotes rolling (4-6). The human alpha 4 ECD shares 85% amino acid sequence identity with the mouse, rat, and canine alpha 4 ECD. The human beta 7 ECD shares 88% and 87% amino acid sequence identity with the rat and mouse beta 7 ECD, respectively. Integrin alpha 4 beta 7 binds the mucosal addressin MAdCAM-1, as well as VCAM-1 and Fibronectin (7). Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes (8, 9). Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid (10, 11). The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) (12-14). Integrin  alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) (15).

1. Luo, B.H. et al. (2007) Annu. Rev. Immunol. 25:619.
2. Takada, Y. et al. (1989) EMBO J. 8:1361.
3. Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.
4. Chen, J. et al. (2004) J. Biol. Chem. 279:55556.
5. Park, E.J. et al. (2007) J. Clin. Invest. 117:2526.
6. Yu, Y. et al. (2012) J. Cell Biol. 196:131.
7. Yang, Y. et al. (1998) Eur. J. Immunol. 28:995.
8. Wagner, N. et al. (1996) Nature 382:366.
9. Johansson-Lindbom, B. and W.W. Agace (2007) Immunol. Rev. 215:226.
10. Hammerschmidt, S.I. et al. (2008) J. Exp. Med. 205:2483.
11. Edele, F. et al. (2008) J. Immunol. 181:3745.
12. Arthos, J. et al. (2008) Nat. Immunol. 9:301.
13. Cicala, C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20877.
14. Monteiro, P. et al. (2011) J. Immunol. 186:4618.
15. Chen, Y.B. et al. (2013) Bone Marrow Transplant. 48:598.

Publications for Integrin alpha 4 beta 7/LPAM-1 (5397-A3)(7)

Publications using 5397-A3

• Su, Y;Luo, Z;Sun, D;Yang, B;Li, Q; The Force-Dependent Mechanism of an Integrin ?4?7-MAdCAM-1 Interaction International journal of molecular sciences 2023-11-07 [PMID: 38003252] (Bioassay, Human)
• I Veisman, O Barzilay, L Bruckmayer, O Haj-Natour, U Kopylov, R Eliakim, S Ben-Horin, B Ungar Association of Infliximab and Vedolizumab Trough Levels with Reported Rates of Adverse Events: A Cross-Sectional Study Journal of Clinical Medicine, 2021-09-20;10(18):. 2021-09-20 [PMID: 34575376] (ELISA Capture, Human)
• PK Myint, A Ito, MG Appiah, G Obeng, S Darkwah, E Kawamoto, A Gaowa, EJ Park, M Shimaoka Irisin supports integrin-mediated cell adhesion of lymphocytes Biochemistry and Biophysics Reports, 2021-03-08;26(0):100977. 2021-03-08 [PMID: 33732908] (ELISA Capture, Human)
• D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells J. Clin. Invest., 2019-05-14;129(7):2745-2759. 2019-05-14 [PMID: 31085833] (Bioassay, Human)
• S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7 PLoS Pathog., 2018-08-28;14(8):e1007278. 2018-08-28 [PMID: 30153309] (Bioassay, Surface Plasmon Resonance, Human)
• JM Caldwell, MH Collins, KA Kemme, JD Sherrill, T Wen, M Rochman, EM Stucke, L Amin, H Tai, PE Putnam, MJ Jim‚nez-Da, MR Wormald, A Porollo, JP Abonia, ME Rothenberg Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation Mucosal Immunol, 2017-01-04;0(0):. 2017-01-04 [PMID: 28051089] (Bioassay, Human, Mouse)
• Guerra-Perez N, Frank I, Veglia F, Aravantinou M, Goode D, Blanchard J, Gettie A, Robbiani M, Martinelli E Retinoic acid imprints a mucosal-like phenotype on dendritic cells with an increased ability to fuel HIV-1 infection. J Immunol, 2015-01-26;194(5):2415-23. 2015-01-26 [PMID: 25624458] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))

Bioinformatics

• Gene Symbol: ITGA4
• Uniprot:
  • P13612 (Integrin a4); P26010 (Integrin beta 7)()