Recombinant Human MXRA8/DICAM Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MXRA8/DICAM Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 2-10 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human MXRA8/DICAM protein
  His23-Gln341, with a C-terminal 6-his tag
• Accession #: Q9BRK3
• N-terminal Sequence: His23 & Ser34
• Protein/Peptide Type: Recombinant Proteins
• Gene: MXRA8
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38-45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MXRA8/DICAM Protein, CF

• Asp3
• DICAM
• Limitrin
• Matrix-Remodeling-Associated Protein 8
• MXRA8

Background

DICAM, also known as Limitrin and MXRA8, is an approximately 70 kDa transmembrane adhesion protein that is expressed on the endothelium of many tissues (1). In the vascular system, it is found on pericytes, vascular endothelial cells (EC), and the vessel-contacting feet of astrocytes (2). It is up-regulated on EC by VEGF and functions as a negative regulator of angiogenesis (3). It inhibits VEGF-induced signaling, cell migration, capillary tube formation, and in vivo angiogenesis, and promotes EC apoptosis (3). The interaction of DICAM with Integrin  alpha V beta 3 enhances cell adhesion and inhibits the differentiation of osteoclasts (1, 4). Mature human DICAM consists of a 322 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and an 80 aa cytoplasmic domain (2). Within the ECD, human DICAM shares 79% aa sequence identity with mouse and rat DICAM. Alternative splicing generates additional isoforms that lack most of the first Ig-like domain or have a substituted signal peptide.

1. Jung, Y.K. et al. (2008) J. Cell Physiol. 216:603.
2. Yonezawa, T. et al. (2003) Glia 44:190.
3. Han, S.-W. et al. (2013) Cardiovasc. Res. 98:73.
4. Jung, Y.-K. et al. (2012) J. Bone Mineral Res. 27:2024.

Bioinformatics

• Gene Symbol: MXRA8
• Uniprot:
  • Q9BRK3()