Recombinant Human R-Spondin 1 Biotinylated Protein

Both Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645) and unlabeled Recombinant Human R-Spondin 1 (Catalog #4645-RS) induces activation of beta -catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human R-Spondin 1 Biotinylated Protein Summary

• Details of Functionality: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 5-30 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
• Source: Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
  Arg31-Ala263
• Accession #: Q2MKA7
• N-terminal Sequence: Ser21 & Arg31
• Structure / Form: Biotinylated protein via sugars
• Protein/Peptide Type: Recombinant Proteins
• Gene: RSPO1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa (unlabeled).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human R-Spondin 1 Biotinylated Protein

• Cristin 3
• CRISTIN3
• FLJ40906Roof plate-specific spondin-1
• HRspo1
• roof plate-specific spondin
• RSPO
• RSPO1
• RSpondin 1
• R-Spondin 1
• R-spondin homolog (Xenopus laevis)
• RSPONDIN
• R-spondin1
• R-spondin-1

Background

R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/ beta -catenin signaling regulators (1). These proteins contain two adjacent cysteine-rich furin-like domains followed by a thrombospondin (TSP-1) motif and a region rich in basic residues. Mature human R-Spondin 1 shares 87% amino acid sequence identity with mouse and rat R-Spondin 1 (2). Alternative splicing generates additional isoforms that have a substituted N-terminus or lack the TSP-1 domain. R-Spondin 1 enhances canonical Wnt/ beta -catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors (3-11). R-Spondin 1 functions in dorsal neural tube development (12) as well as male and female germ cell development (7, 10, 13). It also induces bone formation (6), intestinal crypt cell proliferation (14), angiogenesis (7, 15), and insulin secretion from pancreatic beta cells (11).

1. Jin, Y.R. and J.K. Yoon (2012) Int. J. Biochem. Cell Biol. 44:2278.
2. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
3. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
4. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
5. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
6. Kronke, G. et al. (2010) Arthritis Rheum. 62:2303.
7. Caruso, M. et al. (2015) PLoS One 10:e0124213.
8. Hao, H.-X. et al. (2012) Nature 485:195.
9. de Lau, W. et al. (2011) Nature 476:293.
10. Chassot, A.-A. et al. (2011) PLoS One 6:e25641.
11. Wong, V.S.C et al. (2010) J. Biol. Chem. 285:21292.
12. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
13. Chassost, A.-A. et al. (2012) Development 139:4461.
14. Kim, K.-A. et al. (2005) Science 309:1256.
15. Gore, A.V. et al. (2011) Development 138:4875.

Bioinformatics

• Gene Symbol: RSPO1
• Uniprot:
  • Q2MKA7(Human)