Recombinant Human SCF (Mammalian-expressed) Protein, CF

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL.

Product Discontinued

Recombinant Human SCF (Mammalian-expressed) Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1-5 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human SCF/c-kit Ligand protein
  Glu26-Ala189
• Accession #: P21583
• N-terminal Sequence: Glu26
• Protein/Peptide Type: Recombinant Proteins
• Gene: KITLG
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 24-30 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCF (Mammalian-expressed) Protein, CF

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c‑kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O‑linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel‑dickie mutant mice (11, 12). An alternatively spliced isoform of human SCF exists that lacks 28 aa that encompass the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

1. Ashman, L.K., 1999, Int. J. Biochem. Cell Biol. 31:1037.
2. Sette, C. et al., 2000, Int. J. Dev. Biol. 44:599.
3. Yoshida, H. et al., 2001, J. Invest. Dermatol. Symp. Proc. 6:1.
4. Erlandsson, A. et al., 2004, Exp. Cell Res. 301:201.
5. Kapur, R. et al., 2002, Blood 100:1287.
6. Wang, C.-H. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27:540.
7. Bashamboo, A. et al., 2006, J. Cell Sci. 119:3039.
8. Reber, L. et al., 2006, Eur. J. Pharmacol. 533:327.
9. Martin, F.H. et al., 1990, Cell 63:203.
10. Arakawa, T. et al., 1991, J. Biol. Chem. 266:18942.
11. Majumdar, M.K. et al., 1994, J. Biol. Chem. 269:1237.
12. Brannan, C.I. et al., 1991, Proc. Natl. Acad. Sci. USA 88:4671.
13. 13. Anderson, D.M. et al., 1991, Cell Growth Differ. 2:373.
14. 14. Lemmon, M.A. et al., 1997, J. Biol. Chem. 272:6311.
15. 15. Kanellakis, P. et al., 2006, Cardiovasc. Res. 70:117.

Publications for SCF/c-kit Ligand (7466-SC/CF)(7)

Publications using 7466-SC/CF

• X Wang, S Cooper, HE Broxmeyer, R Kapur Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment Leukemia, 2022-12-02;0(0):. 2022-12-02 [PMID: 36460765] (Cell Culture, Human)
• AF de Souza, FF Bressan, NCG Pieri, RC Botigelli, T Revay, SK Haddad, DT Covas, ES Ramos, WA King, FV Meirelles Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients Cells, 2021-11-10;10(11):. 2021-11-10 [PMID: 34831322] (Bioassay, Human)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020;27(1):125-136.e7. 2020 [PMID: 32579880] (Bioassay, Human)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020-06-19;27(1):125-136.e7. 2020-06-19 [PMID: 32579880] (Bioassay, Human)
• ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019-05-20;130(0):2555-2570. 2019-05-20 [PMID: 31107242] (Bioassay, Human)
• X Huang, B Guo, S Liu, J Wan, HE Broxmeyer Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment Nat Commun, 2018-07-16;9(1):2741. 2018-07-16 [PMID: 30013077] (Bioassay, Human)
• B Guo, X Huang, S Cooper, HE Broxmeyer Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment Nat. Med, 2017-03-06;0(0):. 2017-03-06 [PMID: 28263313] (Bioassay, Human)

Bioinformatics

• Gene Symbol: KITLG
• Uniprot:
  • P21583()