Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 5-15 ng/mL.

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 5-15 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 5‑15 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Thrombopoietin/Tpo protein
  Ser22-Gly353
• Accession #: P40225
• N-terminal Sequence: Ser22
• Protein/Peptide Type: Recombinant Proteins
• Gene: THPO
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF

• Megakaryocyte colony-stimulating factor
• Megakaryocyte growth and development factor
• megakaryocyte stimulating factor
• MGDF
• MGDFC-mpl ligand
• MKCSF
• MK-CSF
• ML
• MPL ligand
• MPLLG
• MPLLGMGC163194
• Myeloproliferative leukemia virus oncogene ligand
• THCYT1
• THPO
• thrombopoietin nirs variant 1
• Thrombopoietin
• Tpo
• TPOMKCSF

Background

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1 - 3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
4. Bartley, T.D. et al. (1994) Cell 77:1117.
5. de Sauvage, F.J. et al. (1994) Nature 369:533.
6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
7. Kaushansky, K. et al. (1994) Nature 369:568.
8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
9. Broudy, V.C. et al. (1995) Blood 85:1719.
10. Lok, S.I. et al. (1994) Nature 369:565.
11. Chen, J. et al. (1995) Blood 86:4054.
12. Oda, A. et al. (1996) Blood 87:4664.
13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

Show all background references

Publications for Thrombopoietin/THPO (288-TPN/CF)(12)

Publications using 288-TPN/CF

• Kunitomi A, Hirohata R, Arreola V, Osawa M, Kato T, Nomura M, Kawaguchi J, Hara H, Kusano K, Takashima Y, Takahashi K, Fukuda K, Takasu N, Yamanaka S Improved Sendai viral system for reprogramming to naive pluripotency. Cell Rep Methods, 2022-10-17;2(11):100317. 2022-10-17 [PMID: 36447645] (Bioassay, Human)
• Sudo K, Yamazaki S, Wilkinson A, Nakauchi H, Nakamura Y Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo. Stem Cell Res, 2021-09-03;56(0):102531. 2021-09-03 [PMID: 34509158] (Bioassay, Human)
• Aerts-Kaya F, Visser T, Pervin B, Mammadova A, Ozyuncu O, Wagemaker G, Uckan-Cetinkaya F SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential. Biol Blood Marrow Transplant, 2020-01-07;26(4):634-642. 2020-01-07 [PMID: 31917271] (Bioassay, Human)
• Ohta R, Sugimura R, Niwa A, Saito M Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition. J Vis Exp, 2019-06-16;0(148):. 2019-06-16 [PMID: 31259914] (Bioassay, Human)
• Niwa A, Heike T, Umeda K A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE, 2011-07-27;6(7):e22261. 2011-07-27 [PMID: 21818303] (Bioassay, Human)
• Uchida N, Hsieh MM, Hayakawa J, Madison C, Washington KN, Tisdale JF Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther., 2011-05-05;18(11):1078-86. 2011-05-05 [PMID: 21544097] (Bioassay, Human)
• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (ELISA (Standard))
• Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008-06-17;36(9):1121-31. 2008-06-17 [PMID: 18562079] (Bioassay, Human)
• Cheli Y, Kanaji S, Jacquelin B, Chang M, Nugent D, Kunicki T Transcriptional and epigenetic regulation of the integrin collagen receptor locus ITGA1-PELO-ITGA2. Biochim Biophys Acta, 2007-07-06;1769(9):546-58. 2007-07-06 [PMID: 17669516] (Bioassay, Human)
• Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007-02-01;25(2):490-9. 2007-02-01 [PMID: 17284653] (Bioassay, Mouse)
• Kanaji S, Kanaji T, Jacquelin B, Chang M, Nugent D, Komatsu N, Moroi M, Izuhara K, Kunicki T Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation. Blood, 2005-02-08;105(10):3888-92. 2005-02-08 [PMID: 15701720] (Bioassay, Human)
• Manz MG, 107325, Miyamoto T, Akashi K, Weissman IL Prospective isolation of human clonogenic common myeloid progenitors. Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases, 2002-08-22;99(18):11872-7. 2002-08-22 [PMID: 12193648] (Bioassay, Human)

Bioinformatics

• Gene Symbol: THPO
• Uniprot:
  • P40225()