Recombinant Human Thrombopoietin Protein

Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-3 ng/mL.

1 µg/lane of Recombinant Human Thrombopoietin was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 43-60 kDa. Multiple bands in gel are due to variable glycosylation.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human Thrombopoietin Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.3-3 ng/mL.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Thrombopoietin/Tpo protein
  Ser22-Gly353
• Accession #: P40225
• N-terminal Sequence: Ser22
• Protein/Peptide Type: Recombinant Proteins
• Gene: THPO
• Purity: >97%, by SDS-PAGE with silver staining
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 43-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombopoietin Protein

• Megakaryocyte colony-stimulating factor
• Megakaryocyte growth and development factor
• megakaryocyte stimulating factor
• MGDF
• MGDFC-mpl ligand
• MKCSF
• MK-CSF
• ML
• MPL ligand
• MPLLG
• MPLLGMGC163194
• Myeloproliferative leukemia virus oncogene ligand
• THCYT1
• THPO
• thrombopoietin nirs variant 1
• Thrombopoietin
• Tpo
• TPOMKCSF

Background

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl. Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O‑linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL‑3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10, 13). It is cleaved by platelet‑derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia‑sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
4. Bartley, T.D. et al. (1994) Cell 77:1117.
5. de Sauvage, F.J. et al. (1994) Nature 369:533.
6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
7. Kaushansky, K. et al. (1994) Nature 369:568.
8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
9. Broudy, V.C. et al. (1995), Blood 85:1719.
10. Lok, S.I. et al. (1994) Nature 369:565.
11. Chen, J. et al. (1995) Blood 86:4054.
12. Oda, A. et al. (1996) Blood 87:4664.
13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

Publications for Thrombopoietin/THPO (288-TP)(91)

Publications using 288-TP

• Demirci, S;Zeng, J;Palchaudhuri, R;Wu, C;Abraham, DM;Hayal, TB;Essawi, K;Nguyen, MA;Stasula, U;Chu, R;Leonard, A;Porter, SN;Khan, MBN;Hinojosa, G;Uchida, N;Hong, S;Lazzarotto, CR;Neri, NR;da Silva, LF;Pellin, D;Verma, A;Lanieri, L;Bhat, A;Hammond, K;Tate, T;Maitland, SA;Sheikhsaran, F;Bonifacino, AC;Krouse, AE;Linde, NS;Engels, T;Golomb, J;Tsai, SQ;Pruett-Miller, SM;Scadden, DT;Dunbar, CE;Wolfe, SA;Donahue, RE;Olson, LM;Bauer, DE;Tisdale, JF; BCL11A +58/+55 enhancer-editing facilitates HSPC engraftment and HbF induction in rhesus macaques conditioned with a CD45 antibody-drug conjugate Cell stem cell 2024-12-05 [PMID: 39642886] (Bioassay, Rhesus Macaque)
• Kent, GM;Atkins, MH;Lung, B;Nikitina, A;Fernandes, IM;Kwan, JJ;Andrews, TS;MacParland, SA;Keller, GM;Gage, BK; Human liver sinusoidal endothelial cells support the development of functional human pluripotent stem cell-derived Kupffer cells Cell reports 2024-08-14 [PMID: 39146183] (Bioassay, Human)
• Mayuranathan, T;Newby, GA;Feng, R;Yao, Y;Mayberry, KD;Lazzarotto, CR;Li, Y;Levine, RM;Nimmagadda, N;Dempsey, E;Kang, G;Porter, SN;Doerfler, PA;Zhang, J;Jang, Y;Chen, J;Bell, HW;Crossley, M;Bhoopalan, SV;Sharma, A;Tisdale, JF;Pruett-Miller, SM;Cheng, Y;Tsai, SQ;Liu, DR;Weiss, MJ;Yen, JS; Potent and uniform fetal hemoglobin induction via base editing Nature genetics 2023-07-03 [PMID: 37400614] (Cell Culture, Human)
• S Li, CS Wang, A Montel-Hag, HC Chen, S Lopez, O Zhou, K Dai, S Tsai, W Satyadi, C Botero, C Wong, D Casero, GM Crooks, CS Seet Strength of CAR signaling determines T�cell versus ILC differentiation from pluripotent stem cells Cell Reports, 2023-03-11;42(3):112241. 2023-03-11 [PMID: 36906850] (Bioassay, Human)
• X Liu, N Sato, T Yabushita, J Li, Y Jia, M Tamura, S Asada, T Fujino, T Fukushima, T Yonezawa, Y Tanaka, T Fukuyama, A Tsuchiya, S Shikata, H Iwamura, C Kinouchi, K Komatsu, S Yamasaki, T Shibata, AT Sasaki, J Schibler, M Wunderlich, E O'Brien, B Mizukawa, JC Mulloy, Y Sugiura, H Takizawa, T Shibata, K Miyake, T Kitamura, S Goyama IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia Embo Molecular Medicine, 2022-12-01;0(0):e15631. 2022-12-01 [PMID: 36453131] (Cell Culture, Human)
• YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022-08-24;8(34):eabn5522. 2022-08-24 [PMID: 36001668] (Bioassay, Human)
• J Skokowa, B Hernandez, M Coles, M Ritter, M Nasri, J Haaf, N Aghaallaei, Y Xu, P Mir, AC Krahl, KW Rogers, K Maksymenko, B Bajoghli, K Welte, AN Lupas, P Müller, M ElGamacy A topological refactoring design strategy yields highly stable granulopoietic proteins Nature Communications, 2022-05-26;13(1):2948. 2022-05-26 [PMID: 35618709] (Bioassay, Human)
• Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis Cell Reports, 2022-05-10;39(6):110805. 2022-05-10 [PMID: 35545056] (Bioassay, Human)
• SS Sahoo, VB Pastor, C Goodings, RK Voss, EJ Kozyra, A Szvetnik, P Noellke, M Dworzak, J Starý, F Locatelli, R Masetti, M Schmugge, B De Moerloo, A Catala, K Kállay, D Turkiewicz, H Hasle, J Buechner, K Jahnukaine, M Ussowicz, S Polychrono, OP Smith, O Fabri, S Barzilai, V de Haas, I Baumann, S Schwarz-Fu, European W, MR Niewisch, MG Sauer, B Burkhardt, P Lang, P Bader, R Beier, I Müller, MH Albert, R Meisel, A Schulz, G Cario, PK Panda, J Wehrle, S Hirabayash, M Derecka, R Durruthy-D, G Göhring, A Yoshimi-No, M Ku, D Lebrecht, M Erlacher, C Flotho, B Strahm, CM Niemeyer, MW Wlodarski Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes Nature Medicine, 2021-10-07;27(10):1806-1817. 2021-10-07 [PMID: 34621053] (Bioassay, Human)
• K Yamamoto, S Goyama, S Asada, T Fujino, T Yonezawa, N Sato, R Takeda, A Tsuchiya, T Fukuyama, Y Tanaka, A Yokoyama, H Toya, A Kon, Y Nannya, R Onoguchi-M, S Nakagawa, T Hirose, S Ogawa, N Akimitsu, T Kitamura A histone modifier, ASXL1, interacts with NONO and is involved in paraspeckle formation in hematopoietic cells Cell Reports, 2021-08-24;36(8):109576. 2021-08-24 [PMID: 34433054] (Bioassay, Human, Mouse)
• H Kato, Y Maezawa, Y Ouchi, N Takayama, M Sone, K Sone, A Takada-Wat, K Tsujimura, M Koshizaka, S Nagasawa, H Saitoh, M Ohtaka, M Nakanishi, H Tahara, A Shimamoto, A Iwama, K Eto, K Yokote Generation of disease-specific and CRISPR/Cas9-mediated gene-corrected iPS cells from a patient with adult progeria Werner syndrome Stem Cell Research, 2021-04-23;53(0):102360. 2021-04-23 [PMID: 34087989] (Cell Culture, Human)
• GA Newby, JS Yen, KJ Woodard, T Mayuranath, CR Lazzarotto, Y Li, H Sheppard-T, SN Porter, Y Yao, K Mayberry, KA Everette, Y Jang, CJ Podracky, E Thaman, C Lechauve, A Sharma, JM Henderson, MF Richter, KT Zhao, SM Miller, T Wang, LW Koblan, AP McCaffrey, JF Tisdale, TA Kalfa, SM Pruett-Mil, SQ Tsai, MJ Weiss, DR Liu Base editing of haematopoietic stem cells rescues sickle cell disease in mice Nature, 2021-06-02;0(0):. 2021-06-02 [PMID: 34079130] (Bioassay, Human)
• S Borst, CC Nations, JG Klein, G Pavani, JA Maguire, RM Camire, MW Drazer, LA Godley, DL French, M Poncz, P Gadue Study of inherited thrombocytopenia resulting from mutations in ETV6 or RUNX1 using a human pluripotent stem cell model Stem Cell Reports, 2021-05-20;16(6):1458-1467. 2021-05-20 [PMID: 34019812] (Bioassay, Human)
• T Wang, AR Pine, AG Kotini, H Yuan, L Zamparo, DT Starczynow, C Leslie, EP Papapetrou Sequential CRISPR gene editing in human iPSCs charts the clonal evolution of myeloid leukemia and identifies early disease targets Cell Stem Cell, 2021-02-10;0(0):. 2021-02-10 [PMID: 33571445] (Bioassay, Human)
• J Chagraoui, S Girard, JF Spinella, L Simon, E Bonneil, N Mayotte, T MacRae, J Coulombe-H, T Bertomeu, C Moison, E Tomellini, P Thibault, M Tyers, A Marinier, G Sauvageau UM171 Preserves Epigenetic Marks that Are Reduced in Ex Vivo Culture of Human HSCs via Potentiation of the CLR3-KBTBD4 Complex Cell Stem Cell, 2021-01-07;28(1):48-62.e6. 2021-01-07 [PMID: 33417871] (Bioassay, Human)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020-06-19;27(1):125-136.e7. 2020-06-19 [PMID: 32579880] (Bioassay, Human)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020;27(1):125-136.e7. 2020 [PMID: 32579880] (Bioassay, Human)
• J Wesely, AG Kotini, F Izzo, H Luo, H Yuan, J Sun, M Georgomano, A Zviran, AG Deslaurier, N Dusaj, SD Nimer, C Leslie, DA Landau, MG Kharas, EP Papapetrou Acute Myeloid Leukemia iPSCs Reveal a Role for RUNX1 in the Maintenance of Human Leukemia Stem Cells Cell Rep, 2020-06-02;31(9):107688. 2020-06-02 [PMID: 32492433] (Cell Culture, Human)
• JL Spivak, A Merchant, DM Williams, O Rogers, W Zhao, A Duffield, LS Resar, AR Moliterno, ZJ Zhao Thrombopoietin is required for full phenotype expression in a JAK2V617F transgenic mouse model of polycythemia vera PLoS ONE, 2020-06-01;15(6):e0232801. 2020-06-01 [PMID: 32479500] (Bioassay, Transgenic Mouse)
• P Norbnop, P Ingrungrua, N Israsena, K Suphapeeti, V Shotelersu Generation and characterization of HLA-universal platelets derived from induced pluripotent stem cells Sci Rep, 2020-05-21;10(1):8472. 2020-05-21 [PMID: 32439978] (Cell Culture, Human)
• Z Zou, X Fan, Y Liu, Y Sun, X Zhang, G Sun, X Li, S Xu Endogenous thrombopoietin promotes non-small-cell lung carcinoma cell proliferation and migration by regulating EGFR signalling J. Cell. Mol. Med., 2020-04-26;0(0):. 2020-04-26 [PMID: 32337844] (Bioassay, Human)
• A Leonard, M Yapundich, T Nassehi, J Gamer, CM Drysdale, JJ Haro-Mora, S Demirci, MM Hsieh, N Uchida, JF Tisdale Low-Dose Busulfan Reduces Human CD34+ Cell Doses Required for Engraftment in c-kit Mutant Immunodeficient Mice Mol Ther Methods Clin Dev, 2019-11-11;15(0):430-437. 2019-11-11 [PMID: 31890735] (Bioassay, Human)
• T Fukushima, Y Tanaka, FK Hamey, CH Chang, T Oki, S Asada, Y Hayashi, T Fujino, T Yonezawa, R Takeda, KC Kawabata, T Fukuyama, T Umemoto, K Takubo, H Takizawa, S Goyama, Y Ishihama, H Honda, B Göttgens, T Kitamura Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium Cell Rep, 2019-12-17;29(12):4144-4158.e7. 2019-12-17 [PMID: 31851939] (Cell Culture, Mouse)
• J Chagraoui, B Lehnertz, S Girard, JF Spinella, I Fares, E Tomellini, N Mayotte, S Corneau, T MacRae, L Simon, G Sauvageau UM171 induces a homeostatic inflammatory-detoxification response supporting human HSC self-renewal PLoS ONE, 2019-11-08;14(11):e0224900. 2019-11-08 [PMID: 31703090] (Cell Culture, Human)
• J Grajcarek, J Monlong, Y Nishinaka-, M Nakamura, M Nagai, S Matsuo, D Lougheed, H Sakurai, MK Saito, G Bourque, K Woltjen Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations Nat Commun, 2019-10-24;10(1):4856. 2019-10-24 [PMID: 31649251] (Bioassay, Human)
• N Uchida, MM Hsieh, L Raines, JJ Haro-Mora, S Demirci, AC Bonifacino, AE Krouse, ME Metzger, RE Donahue, JF Tisdale Development of a forward-oriented therapeutic lentiviral vector for hemoglobin disorders Nat Commun, 2019-10-02;10(1):4479. 2019-10-02 [PMID: 31578323] (Cell Culture, Human)
• FJ Román-Rodr, L Ugalde, L Álvarez, B Díez, MJ Ramírez, C Risueño, M Cortón, M Bogliolo, S Bernal, F March, C Ayuso, H Hanenberg, J Sevilla, S Rodríguez-, R Torres-Rui, J Surrallés, JA Bueren, P Río NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia Cell Stem Cell, 2019-09-19;0(0):. 2019-09-19 [PMID: 31543367] (Bioassay, Human)
• GL Rogers, HY Chen, H Morales, PM Cannon Homologous Recombination-Based Genome Editing by Clade F AAVs Is Inefficient in the Absence of a Targeted DNA Break Mol. Ther., 2019-09-09;27(10):1726-1736. 2019-09-09 [PMID: 31540849] (Bioassay, Human)
• E Tomellini, I Fares, B Lehnertz, J Chagraoui, N Mayotte, T MacRae, MÈ Bordeleau, S Corneau, R Bisaillon, G Sauvageau Integrin-α3 Is a Functional Marker of Ex�Vivo Expanded Human Long-Term Hematopoietic Stem Cells Cell Rep, 2019-07-23;28(4):1063-1073.e5. 2019-07-23 [PMID: 31340144] (Cell Culture, Human)
• MA Kinney, LT Vo, JM Frame, J Barragan, AJ Conway, S Li, KK Wong, JJ Collins, P Cahan, TE North, DA Lauffenbur, GQ Daley A systems biology pipeline identifies regulatory networks for stem cell engineering Nat. Biotechnol., 2019-07-01;37(7):810-818. 2019-07-01 [PMID: 31267104] (Cell Culture, Human)
• M Nasri, M Ritter, P Mir, B Dannenmann, N Aghaallaei, D Amend, V Makaryan, Y Xu, B Fletcher, R Bernhard, I Steiert, K Hahnel, J Berger, I Koch, B Sailer, K Hipp, C Zeidler, M Klimiankou, B Bajoghli, DC Dale, K Welte, J Skokowa CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients Haematologica, 2019-06-27;0(0):. 2019-06-27 [PMID: 31248972] (Bioassay, Human)
• ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019-05-20;130(0):2555-2570. 2019-05-20 [PMID: 31107242] (Bioassay, Human)
• M Ruiz-Gutie, ÖV Bölükba??, G Alexe, AG Kotini, K Ballotti, CE Joyce, DW Russell, K Stegmaier, K Myers, CD Novina, EP Papapetrou, A Shimamura Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells JCI Insight, 2019-04-30;5(0):. 2019-04-30 [PMID: 31039138] (Cell Culture, Human)
• B Mair, J Tomic, SN Masud, P Tonge, A Weiss, M Usaj, AHY Tong, JJ Kwan, KR Brown, E Titus, M Atkins, KSK Chan, L Munsie, A Habsid, H Han, M Kennedy, B Cohen, G Keller, J Moffat Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies Cell Rep, 2019-04-09;27(2):599-615.e12. 2019-04-09 [PMID: 30970261] (Differentiation, Differentiation, Human)
• M Suga, T Kondo, K Imamura, R Shibukawa, Y Okanishi, Y Sagara, K Tsukita, T Enami, M Furujo, K Saijo, Y Nakamura, M Osawa, MK Saito, S Yamanaka, H Inoue Generation of a human induced pluripotent stem cell line, BRCi001-A, derived from a patient with mucopolysaccharidosis type I Stem Cell Res, 2019-02-12;36(0):101406. 2019-02-12 [PMID: 30849633] (Bioassay, Human)
• N Uchida, T Nassehi, CM Drysdale, J Gamer, M Yapundich, S Demirci, JJ Haro-Mora, A Leonard, MM Hsieh, JF Tisdale High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2 Mol Ther Methods Clin Dev, 2019-01-25;13(0):187-196. 2019-01-25 [PMID: 30788387] (Bioassay, Human)
• M Kaur, AC Drake, G Hu, S Rudnick, Q Chen, R Phennicie, R Attar, J Nemeth, F Gaudet, J Chen Induction and Therapeutic Targeting of Human NPM1c+ Myeloid Leukemia in the Presence of Autologous Immune System in Mice J. Immunol., 2019-02-01;0(0):. 2019-02-01 [PMID: 30710044] (Bioassay, Human)
• X Huang, B Guo, S Liu, J Wan, HE Broxmeyer Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment Nat Commun, 2018-07-16;9(1):2741. 2018-07-16 [PMID: 30013077] (Bioassay, Mouse)
• A Camgoz, M Paszkowski, S Satpathy, M Wermke, MV Hamann, M von Bonin, C Choudhary, S Knapp, F Buchholz STK3 is a therapeutic target for a subset of acute myeloid leukemias Oncotarget, 2018-05-22;9(39):25458-25473. 2018-05-22 [PMID: 29876001] (Bioassay, Human)
• M Cesana, MH Guo, D Cacchiarel, L Wahlster, J Barragan, S Doulatov, LT Vo, B Salvatori, C Trapnell, K Clement, P Cahan, KM Tsanov, PM Sousa, B Tazon-Vega, A Bolondi, FM Giorgi, A Califano, JL Rinn, A Meissner, JN Hirschhorn, GQ Daley A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development Cell Stem Cell, 2018-04-05;22(4):575-588.e7. 2018-04-05 [PMID: 29625070] (Bioassay, Human)
• E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia Blood Adv, 2018-04-10;2(7):754-761. 2018-04-10 [PMID: 29599195] (Cell Culture, Human)
• S Zheng, E Papalexi, A Butler, W Stephenson, R Satija Molecular transitions in early progenitors during human cord blood hematopoiesis Mol. Syst. Biol., 2018-03-15;14(3):e8041. 2018-03-15 [PMID: 29545397] (Bioassay, Human)
• A Palau, AK Garz, J Diesch, A Zwick, R Malinverni, V Valero, K Lappin, R Casquero, A Lennartsso, J Zuber, T Navarro, KI Mills, KS Götze, M Buschbeck Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes Oncotarget, 2017-12-01;8(70):115002-115017. 2017-12-01 [PMID: 29383137] (Bioassay, Human)
• NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis Cell Rep, 2017-08-15;20(7):1597-1608. 2017-08-15 [PMID: 28813672] (Bioassay, Human)
• JH Lee, KR Salci, JC Reid, L Orlando, B Tanasijevi, Z Shapovalov, M Bhatia Brief Report: Human Acute Myeloid Leukemia Reprograming to Pluripotency Is a Rare Event and Selects for Patient Hematopoietic Cells Devoid of Leukemic Mutations Stem Cells, 2017-07-31;0(0):. 2017-07-31 [PMID: 28758276] (Bioassay, Human)
• J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease Mol Ther Methods Clin Dev, 2017-05-12;5(0):241-258. 2017-05-12 [PMID: 28603745] (Bioassay, Human)
• R Sugimura, DK Jha, A Han, C Soria-Vall, EL da Rocha, YF Lu, JA Goettel, E Serrao, RG Rowe, M Malleshaia, I Wong, P Sousa, TN Zhu, A Ditadi, G Keller, AN Engelman, SB Snapper, S Doulatov, GQ Daley Haematopoietic stem and progenitor cells from human pluripotent stem cells Nature, 2017-05-17;545(7655):432-438. 2017-05-17 [PMID: 28514439] (Bioassay, Human)
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• Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes Immunology, 2016-09-30;0(0):. 2016-09-30 [PMID: 27568595] (Bioassay, Human)
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• Kazuki , Yasuhiro, Yakura , Yuwna, Abe , Satoshi, Osaki , Mitsuhik, Kajitani , Naoyo, Kazuki , Kanako, Takehara , Shoko, Honma , Kazuhisa, Suemori , Hirofumi, Yamazaki , Satoshi, Sakuma , Tetsushi, Toki , Tsutomu, Shimizu , Ritsuko, Nakauchi , Hiromits, Yamamoto , Takashi, Oshimura , Mitsuo Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies. Sci Rep, 2014-08-27;4(0):6136. 2014-08-27 [PMID: 25159877] (Bioassay, Human)
• Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood, 2014-07-02;124(9):1393-403. 2014-07-02 [PMID: 24990886] (Bioassay, Mouse)
• Rosen , David B, Leung , Ling Y, Louie , Brent, Cordeiro , James A, Conroy , Andrew, Shapira , Iuliana, Fields , Scott Z, Cesano , Alessand, Hawtin , Rachael Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP). J Transl Med, 2014-06-25;12(0):184. 2014-06-25 [PMID: 24965603] (Bioassay, Human)
• Chaurasia P, Gajzer D, Schaniel C, D'Souza S, Hoffman R Epigenetic reprogramming induces the expansion of cord blood stem cells. J Clin Invest, 2014-04-24;124(6):2378-95. 2014-04-24 [PMID: 24762436] (Bioassay, Human)
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• Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K, Koike T, Harimoto K, Dohda T, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell, 2014-02-13;14(4):535-48. 2014-02-13 [PMID: 24529595] (Bioassay, Human)
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• Sjeklocha L, Wong P, Belcher J, Vercellotti G, Steer C beta-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model. PLoS ONE, 2013-11-18;8(11):e80403. 2013-11-18 [PMID: 24260386] (Cell Culture, Human)
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• Csaszar , Elizabet, Wang , Weijia, Usenko , Tatiana, Qiao , Wenlian, Delaney , Colleen, Bernstein , Irwin D, Zandstra , Peter W Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling. Blood, 2013-11-15;123(5):650-8. 2013-11-15 [PMID: 24243972] (Bioassay, Human)
• Shinohara A, Imai Y, Nakagawa M, Takahashi T, Ichikawa M, Kurokawa M Intracellular reactive oxygen species mark and influence the megakaryocyte-erythrocyte progenitor fate of common myeloid progenitors. Stem Cells, 2014-02-01;32(2):548-57. 2014-02-01 [PMID: 24167091] (Bioassay, Mouse)
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• Dasouki , Majed J, Rafi , Syed K, Olm-Shipman , Adam J, Wilson , Nathan R, Abhyankar , Sunil, Ganter , Brigitte, Furness , L Mike, Fang , Jianwen, Calado , Rodrigo, Saadi , Irfan Exome sequencing reveals a thrombopoietin ligand mutation in a Micronesian family with autosomal recessive aplastic anemia. Blood, 2013-10-01;122(20):3440-9. 2013-10-01 [PMID: 24085763] (Cell Culture, Human)
• Giannoni F, Hardee C, Wherley J, Gschweng E, Senadheera S, Kaufman M, Chan R, Bahner I, Gersuk V, Wang X, Gjertson D, Baltimore D, Witte O, Economou J, Ribas A, Kohn D Allelic exclusion and peripheral reconstitution by TCR transgenic T cells arising from transduced human hematopoietic stem/progenitor cells. Mol Ther, 2013-02-05;21(5):1044-54. 2013-02-05 [PMID: 23380815] (Bioassay, Human)
• Lee M, Prasain N, Chae H, Kim Y, Mantel C, Yoder M, Broxmeyer H Epigenetic regulation of NANOG by miR-302 cluster-MBD2 completes induced pluripotent stem cell reprogramming. Stem Cells, 2013-04-01;31(4):666-81. 2013-04-01 [PMID: 23255147] (Bioassay, Human)
• Targeting of CD34+CD38- cells using Gemtuzumab ozogamicin (Mylotarg) in combination with tipifarnib (Zarnestra) in Acute Myeloid Leukaemia. BMC Cancer, 2012-09-26;12(0):431. 2012-09-26 [PMID: 23013471] (Bioassay, Human)
• Mcl-1 and Bcl-xL regulate Bak/Bax-dependent apoptosis of the megakaryocytic lineage at multistages. Cell Death Differ., 2012-07-13;19(11):1856-69. 2012-07-13 [PMID: 22790873] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
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• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (ELISA (Standard))
• Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 2008-05-27;36(8):1035-46. 2008-05-27 [PMID: 18504067] (Bioassay, Human)
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• Kurita R, Sasaki E, Yokoo T, Hiroyama T, Takasugi K, Imoto H, Izawa K, Dong Y, Hashiguchi T, Soda Y, Maeda T, Suehiro Y, Tanioka Y, Nakazaki Y, Tani K Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells. Stem Cells, 2006-05-25;24(9):2014-22. 2006-05-25 [PMID: 16728561] (Bioassay, Primate - Callitrix jacchus (Common Marmoset))
• Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells. Exp. Hematol., 2006-06-01;34(6):760-9. 2006-06-01 [PMID: 16728281] (Bioassay, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Kahl CA, Tarantal AF, Lee CI, Jimenez DF, Choi C, Pepper K, Petersen D, Fletcher MD, Leapley AC, Fisher J, Burns TS, Ultsch MN, Dorey FJ, Kohn DB Effects of busulfan dose escalation on engraftment of infant rhesus monkey hematopoietic stem cells after gene marking by a lentiviral vector. Exp. Hematol., 2006-03-01;34(3):369-81. 2006-03-01 [PMID: 16543071] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))
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Bioinformatics

• Gene Symbol: THPO
• Uniprot:
  • P40225()