Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 0.2-0.6 µg/mL in the presence of 0.25 ng/mL rhTNF-alpha .
• Source: E. coli-derived human TNF RII/TNFRSF1B protein
  Pro24-Thr206
• Accession #: AAA36755
• N-terminal Sequence: Pro24
• Protein/Peptide Type: Recombinant Proteins
• Gene: TNFRSF1B
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein, CF

• CD120b antigen
• CD120b
• Etanercept
• p75 TNF receptor
• p75TBPII
• p75TNFR
• soluble TNFR1B variant 1
• TNF RII
• TNFBRp80 TNF-alpha receptor
• TNF-R2
• TNFR2TNFR1B
• TNF-R75
• TNFR80
• TNFRII
• TNF-RII
• TNF-R-II
• TNFR-II
• TNFRSF1B
• tumor necrosis factor beta receptor
• tumor necrosis factor binding protein 2
• Tumor necrosis factor receptor 2
• tumor necrosis factor receptor superfamily member 1B
• tumor necrosis factor receptor superfamily, member 1B
• Tumor necrosis factor receptor type II

Background

TNF RI (also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with high-affinity. Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. The extracellular domains of both receptors contain four conserved cysteine-rich domains while their intracellular domains are dissimilar, indicative of distinct signaling pathways. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. Whereas all cell types studied express TNF RI, TNF RII expression is limited primarily to hematopoietic cells and cells of the immune system. Most of the biological functions of TNF are mediated via TNF RI. Soluble forms of both TNF RI and TNF RII, generated as a result of proteolytic cleavage of the extracellular domains, have been detected in vivo in various biological fluids. The soluble TNF receptors can bind TNF with high affinity and function as TNF antagonists (1 - 4).

1. Chen G. and D.V. Goeddel (2002) Science 296:1634.
2. MacEwan, D.J. (2002) Cell Signal. 14:477.
3. Locksley, R.M. et al. (2001) Cell 104:487.
4. Feldmann, M.and R.N. Maini (2001) Annu. Rev. Immunol. 19:163.

Publications for TNF RII/TNFRSF1B (1089-R2/CF)(4)

Publications using 1089-R2/CF

• T Baram, N Oren, N Erlichman, T Meshel, A Ben-Baruch Inflammation-Driven Regulation of PD-L1 and PD-L2, and Their Cross-Interactions with Protective Soluble TNFalpha Receptors in Human Triple-Negative Breast Cancer Cancers, 2022-07-19;14(14):. 2022-07-19 [PMID: 35884574] (Bioassay, Human)
• BH Clausen, M Wirenfeldt, SS Høgedal, LH Frich, HH Nielsen, HD Schrøder, K Østergaard, B Finsen, BW Kristensen, KL Lambertsen Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke Acta Neuropathol Commun, 2020-06-05;8(1):81. 2020-06-05 [PMID: 32503645] (Bioassay, Human)
• Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1. J Biol Chem, 2014-12-23;290(7):4022-37. 2014-12-23 [PMID: 25538244] (Enzyme Assay, Human)
• Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-01-01;284(1):99-106. 2004-01-01 [PMID: 14736420] (ELISA (Standard))

Bioinformatics

• Gene Symbol: TNFRSF1B
• Uniprot:
  • AAA36755()