Recombinant Mouse IL-7 Protein

Recombinant Mouse IL-7 (Catalog # 407‑ML) stimulates cell proliferation of PHA-activated human peripheral blood lymphocytes. The ED50 for this effect is 0.15-0.3 ng/mL.

Recombinant Mouse IL-7 (Catalog # 407‑ML) stimulates cell proliferation of PHA-activated human peripheral blood lymphocytes. The ED50 for this effect is 0.15-0.3 ng/mL.

1 μg/lane of Recombinant Mouse IL-7 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse IL-7 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED₅₀ for this effect is 0.15-0.3 ng/mL.
• Source: E. coli-derived mouse IL-7 protein
  Glu26-Ile154, with an N-terminal Met
• Accession #: Q544C8
• N-terminal Sequence: Glu26
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il7
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-7 Protein

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). Mouse IL-7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide (4). Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha , also known as CD127) with the common gamma chain ( gamma _c) (1). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
4. Namen, A.E. et al. (1988) Nature 333:571.
5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
6. Saini, M. et al. (2009) Blood 113:5793.
7. Park, J.H. et al. (2004) Immunity 21:289.
8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
15. Nodland, S.E. et al. (2011) Blood 118:2116.

Show all background references

Publications for IL-7 (407-ML)(109)

Publications using 407-ML

• Durand, A;Bonilla, N;Level, T;Ginestet, Z;Lombès, A;Guichard, V;Germain, M;Jacques, S;Letourneur, F;Do Cruzeiro, M;Marchiol, C;Renault, G;Le Gall, M;Charvet, C;Le Bon, A;Martin, B;Auffray, C;Lucas, B; Type 1 interferons and Foxo1 down-regulation play a key role in age-related T-cell exhaustion in mice Nature communications 2024-02-26 [PMID: 38409097] (Bioassay, Mouse)
• Zheng, M;Kim, MH;Park, SG;Kim, WS;Oh, SH;Sung, JH; CXCL12 Neutralizing Antibody Promotes Hair Growth in Androgenic Alopecia and Alopecia Areata International journal of molecular sciences 2024-01-30 [PMID: 38338982] (Bioassay, Mouse)
• Read, E;Peña-Cearra, A;Coman, D;Jowett, GM;W H Chung, M;Coales, I;Syntaka, S;Finlay, RE;Tachó-Piñot, R;van Der Post, S;Naizi, U;Roberts, LB;Hepworth, MR;Curtis, MA;Neves, JF; Bi-directional signalling between the intestinal epithelium and type-3 innate lymphoid cells (ILC3) regulates secretory dynamics and IL-22 Mucosal immunology 2023-11-10 [PMID: 37952849] (Bioassay, Mouse)
• Su, HH;Cheng, CM;Yang, YN;Chang, YW;Li, CY;Wu, ST;Lin, CC;Wu, HE;Suen, JL; Acrylamide, an Air Pollutant, Enhances Allergen-Induced Eosinophilic Lung Inflammation via Group 2 Innate Lymphoid Cells Mucosal immunology 2023-10-05 [PMID: 37805143] (Bioassay, Mouse)
• TE Billipp, C Fung, LM Webeck, DB Sargent, MB Gologorsky, MM McDaniel, DN Kasal, JW McGinty, KA Barrow, LM Rich, A Barilli, M Sabat, JS Debley, R Myers, MR Howitt, J von Moltke Tuft cell-derived acetylcholine regulates epithelial fluid secretion bioRxiv : the preprint server for biology, 2023-03-22;0(0):. 2023-03-22 [PMID: 36993541] (Bioassay, Mouse)
• RK Gurram, D Wei, Q Yu, MJ Butcher, X Chen, K Cui, G Hu, M Zheng, X Zhu, J Oh, B Sun, JF Urban, K Zhao, WJ Leonard, J Zhu Crosstalk between ILC2s and Th2 cells varies among mouse models Cell Reports, 2023-02-02;42(2):112073. 2023-02-02 [PMID: 36735533] (Bioassay, Mouse)
• MR Rollins, JF Raynor, EA Miller, JZ Butler, EJ Spartz, WS Lahr, Y You, AL Burrack, BS Moriarity, BR Webber, IM Stromnes Germline T cell receptor exchange results in physiological T cell development and function Nature Communications, 2023-02-01;14(1):528. 2023-02-01 [PMID: 36726009] (Bioassay, Mouse)
• C Jacoberger, I Cousineau, Y Bareche, D Allard, P Chrobak, B Allard, S Pommey, N Messaoudi, Y McNicoll, G Soucy, S Koseoglu, R Masia, AC Lake, H Seo, CB Eeles, N Rohatgi, SC Robson, S Turcotte, B Haibe-Kain, J Stagg CD73 inhibits cGAS-STING and cooperates with CD39 to promote pancreatic cancer Cancer Immunology Research, 2023-01-03;0(0):. 2023-01-03 [PMID: 36409930] (Bioassay, Transgenic Mouse)
• N Sugitani, FP Vendetti, AJ Cipriano, P Pandya, JJ Deppas, TN Moiseeva, S Schamus-Ha, Y Wang, D Palmer, HU Osmanbeyog, A Bostwick, NW Snyder, YN Gong, KM Aird, GM Delgoffe, JH Beumer, CJ Bakkenist Thymidine rescues ATR kinase inhibitor-induced deoxyuridine contamination in genomic DNA, cell death, and interferon-alpha/beta expression Cell Reports, 2022-09-20;40(12):111371. 2022-09-20 [PMID: 36130512] (Bioassay, Mouse)
• JH Badrani, AN Strohm, L Lacasa, B Civello, K Cavagnero, YA Haung, M Amadeo, LH Naji, SJ Lund, A Leng, H Kim, RE Baum, N Khorram, M Mondal, G Seumois, J Pilotte, PW Vanderklis, HM McGee, TA Doherty RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R Nature Communications, 2022-07-30;13(1):4435. 2022-07-30 [PMID: 35908044] (Bioassay, Mouse)
• HS Lee, HW Park IL-23 plays a significant role in the augmentation of particulate matter-mediated allergic airway inflammation Journal of Cellular and Molecular Medicine, 2022-07-08;0(0):. 2022-07-08 [PMID: 35801505] (Bioassay, Mouse)
• N Baran, A Lodi, Y Dhungana, S Herbrich, M Collins, S Sweeney, R Pandey, A Skwarska, S Patel, M Tremblay, VM Kuruvilla, A Cavazos, M Kaplan, MO Warmoes, DT Veiga, K Furudate, S Rojas-Sutt, A Haman, Y Gareau, A Marinier, H Ma, K Harutyunya, M Daher, LM Garcia, G Al-Atrash, S Piya, V Ruvolo, W Yang, SS Shanmugave, N Feng, J Gay, D Du, JJ Yang, FW Hoff, M Kaminski, K Tomczak, R Eric Davis, D Herranz, A Ferrando, EJ Jabbour, M Emilia Di, DT Teachey, TM Horton, S Kornblau, K Rezvani, G Sauvageau, M Gagea, M Andreeff, K Takahashi, JR Marszalek, PL Lorenzi, J Yu, S Tiziani, T Hoang, M Konopleva Inhibition of mitochondrial complex I reverses NOTCH1-driven metabolic reprogramming in T-cell acute lymphoblastic leukemia Nature Communications, 2022-05-19;13(1):2801. 2022-05-19 [PMID: 35589701] (Bioassay, Mouse)
• EA Kuczynski, G Morlino, A Peter, AML Coenen-Sta, JI Moss, N Wali, O Delpuech, A Reddy, A Solanki, C Sinclair, DP Calado, LS Carnevalli A preclinical model of peripheral T-cell lymphoma GATA3 reveals DNA damage response pathway vulnerability Embo Molecular Medicine, 2022-05-05;0(0):e15816. 2022-05-05 [PMID: 35510955] (Bioassay, Mouse)
• J Jeong, I Jung, JH Kim, S Jeon, DY Hyeon, H Min, B Kang, J Nah, D Hwang, SJ Um, M Ko, RH Seong BAP1 shapes the bone marrow niche for lymphopoiesis by fine-tuning epigenetic profiles in endosteal mesenchymal stromal cells Cell Death and Differentiation, 2022-04-26;0(0):. 2022-04-26 [PMID: 35473985] (Bioassay, Mouse)
• P Prakhar, J Alvarez-De, H Keller, A Crossman, X Tai, YK Park, JH Park The small intestine epithelium exempts Foxp3+ Tregs from their IL-2 requirement for homeostasis and effector function JCI Insight, 2021-11-08;6(21):. 2021-11-08 [PMID: 34747370] (Bioassay, Mouse)
• TT Vo, LO Herzog, R Buono, JS Lee, S Mallya, MR Duong, J Thao, M Gotesman, DA Fruman Targeting eIF4F translation complex sensitizes B-ALL cells to tyrosine kinase inhibition Scientific Reports, 2021-11-04;11(1):21689. 2021-11-04 [PMID: 34737376] (Bioassay, Mouse)
• ME Kotas, NM Mroz, S Koga, HE Liang, AW Schroeder, RR Ricardo-Go, C Schneider, RM Locksley CISH constrains the tuft-ILC2 circuit to set epithelial and immune tone Mucosal Immunology, 2021-07-21;0(0):. 2021-07-21 [PMID: 34290377] (Bioassay, Mouse)
• A Srinivasan, S Bajana, A Pankow, C Yuen, RK Shah, XH Sun Type 2 innate lymphoid cells from Id1 transgenic mice alleviate skin manifestations of graft-versus-host disease BMC immunology, 2021-07-13;22(1):46. 2021-07-13 [PMID: 34256699] (Bioassay, Mouse)
• P de Lucía F, C Sherwood, I Saranchova, W Xia, L Munro, CG Pfeifer, JM Piret, WA Jefferies Serum free culture for the expansion and study of type 2 innate lymphoid cells Scientific Reports, 2021-06-10;11(1):12233. 2021-06-10 [PMID: 34112824] (Cell Culture, Mouse)
• CH Hsieh, HC Hsieh, FS Shih, PW Wang, LX Yang, DB Shieh, YC Wang An innovative NRF2 nano-modulator induces lung cancer ferroptosis and elicits an immunostimulatory tumor microenvironment Theranostics, 2021-05-13;11(14):7072-7091. 2021-05-13 [PMID: 34093872] (Bioassay, Mouse)
• K Hashimoto, Y Yamada, K Sekiguchi, S Matsuda, S Mori, T Matsumoto Induction of alopecia areata in C3H/HeJ mice using cryopreserved lymphocytes Journal of dermatological science, 2021-05-04;0(0):. 2021-05-04 [PMID: 33994063] (Bioassay, Mouse)
• J Lu, S Li, X Li, W Zhao, X Duan, X Gu, J Xu, B Yu, LJ Sigal, Z Dong, L Xie, M Fang Declined miR-181a-5p expression is associated with impaired natural killer cell development and function with aging Aging Cell, 2021-03-29;0(0):e13353. 2021-03-29 [PMID: 33780118] (Bioassay, Mouse)
• SP Fahl, AV Contreras, A Verma, X Qiu, C Harly, F Radtke, JC Zúñiga-Pfl, C Murre, HH Xue, JM Sen, DL Wiest The E protein-TCF1 axis controls &amp;gamma&amp;delta T�cell development and effector fate Cell Reports, 2021-02-02;34(5):108716. 2021-02-02 [PMID: 33535043] (Bioassay, Mouse)
• Y Hong, N Singh, S Bamopoulos, E Gjerga, LK Schmalbroc, K Balabanian, M Schick, U Keller, M Wirth Authentication of Primary Murine Cell Lines by a Microfluidics-Based Lab-On-Chip System Biomedicines, 2020-12-09;8(12):. 2020-12-09 [PMID: 33317212] (Bioassay, Mouse)
• M Isidro-Her, A Mayado, A Casado-Gar, J Martínez-C, C Palmi, G Fazio, A Orfao, J Ribera, JM Ribera, L Zamora, J Raboso-Gal, O Blanco, D Alonso-Lóp, J De Las Riv, R Jiménez, FJ García Cri, MB García Cen, M Ramírez-Or, G Cazzaniga, C Cobaleda, C Vicente-Du, I Sánchez-Ga Inhibition of inflammatory signaling in Pax5 mutant cells mitigates B-cell leukemogenesis Sci Rep, 2020-11-05;10(1):19189. 2020-11-05 [PMID: 33154497] (Cell Culture, Mouse)
• V Peng, C Georgescu, A Bakowska, A Pankow, L Qian, JD Wren, XH Sun E proteins orchestrate dynamic transcriptional cascades implicated in the suppression of the differentiation of group 2 innate lymphoid cells J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32817168] (Bioassay, Cell Culture, Mouse)
• JM Inclan-Ric, JJ Ponessa, N Valero-Pac, CM Hernandez, CB Sy, AD Lemenze, AM Beaulieu, MC Siracusa Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition Nat. Immunol., 2020-08-17;0(0):. 2020-08-17 [PMID: 32807943] (Bioassay, Mouse)
• S Valletta, A Thomas, Y Meng, X Ren, R Drissen, H Sengül, C Di Genua, C Nerlov Micro-environmental sensing by bone marrow stroma identifies IL-6 and TGFbeta1 as regulators of hematopoietic ageing Nat Commun, 2020-08-14;11(1):4075. 2020-08-14 [PMID: 32796847] (Bioassay, Mouse)
• S Steinmann, M Schoedsack, F Heinrich, PC Breda, A Ochel, G Tiegs, K Neumann Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells Sci Rep, 2020-01-23;10(1):1071. 2020-01-23 [PMID: 31974518] (Cell Culture, Mouse)
• OO Oyesola, C Duque, LC Huang, EM Larson, SP Früh, LM Webb, SA Peng, ED Tait Wojno The Prostaglandin D2 Receptor CRTH2 Promotes IL-33-Induced ILC2 Accumulation in the Lung J. Immunol., 2020-01-03;204(4):1001-1011. 2020-01-03 [PMID: 31900341] (Cell Culture, Mouse)
• PT Mantani, P Dunér, I Ljungcrant, J Nilsson, H Björkbacka, GN Fredrikson ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions BMC Immunol., 2019-12-10;20(1):47. 2019-12-10 [PMID: 31823769] (Bioassay, Mouse)
• ELY Chen, PK Thompson, JC Zúñiga-Pfl RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors Nat. Immunol., 2019-10-21;0(0):. 2019-10-21 [PMID: 31636466] (Cell Culture, Mouse)
• N Ron-Harel, JM Ghergurovi, G Notarangel, MW LaFleur, Y Tsubosaka, AH Sharpe, JD Rabinowitz, MC Haigis T Cell Activation Depends on Extracellular Alanine Cell Rep, 2019-09-17;28(12):3011-3021.e4. 2019-09-17 [PMID: 31533027] (Bioassay, Mouse)
• M Mandal, MK Okoreeh, DE Kennedy, M Maienschei, J Ai, KC McLean, N Kaverina, M Veselits, I Aifantis, F Gounari, MR Clark CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis Nat. Immunol., 2019-09-02;0(0):. 2019-09-02 [PMID: 31477919] (Bioassay, Mouse)
• T Sasaki, K Moro, T Kubota, N Kubota, T Kato, H Ohno, S Nakae, H Saito, S Koyasu Innate Lymphoid Cells in the Induction of Obesity Cell Rep, 2019-07-02;28(1):202-217.e7. 2019-07-02 [PMID: 31269440] (Bioassay, Mouse)
• C Symowski, D Voehringer Th2 cell-derived IL-4/IL-13 promote ILC2 accumulation in the lung by ILC2-intrinsic STAT6 signaling in mice Eur. J. Immunol., 2019-06-05;0(0):. 2019-06-05 [PMID: 31144294] (Bioassay, Mouse)
• B Maurer, H Nivarthi, B Wingelhofe, HTT Pham, M Schlederer, T Suske, R Grausenbur, AI Schiefer, M Prchal-Mur, D Chen, S Winkler, O Merkel, C Kornauth, M Hofbauer, B Hochgatter, G Hoermann, A Hoelbl-Kov, J Prochazkov, C Lobello, AA Cumaraswam, J Latzka, M Kitzwögere, A Chott, A Janikova, Š Pospíšilov, JI Loizou, S Kubicek, P Valent, T Kolbe, F Grebien, L Kenner, PT Gunning, R Kralovics, M Herling, M Müller, T Rülicke, V Sexl, R Moriggl High activation of STAT5A drives peripheral T-cell lymphoma and leukemia Haematologica, 2020-01-31;0(0):. 2020-01-31 [PMID: 31123029] (Bioassay, Mouse)
• DM Previte, CP Martins, EC O'Connor, ML Marre, GM Coudriet, NW Beck, AV Menk, RH Wright, HM Tse, GM Delgoffe, JD Piganelli Lymphocyte Activation Gene-3 Maintains Mitochondrial and Metabolic Quiescence in Naive CD4+ T Cells Cell Rep, 2019-04-02;27(1):129-141.e4. 2019-04-02 [PMID: 30943396] (Bioassay, Mouse)
• S Goossens, J Wang, C Tremblay, J De Medts, S T'Sas, T Nguyen, J Saw, K Haigh, DJ Curtis, P Van Vlierb, G Berx, T Taghon, JJ Haigh ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms Haematologica, 2019-01-24;0(0):. 2019-01-24 [PMID: 30679322] (Bioassay, Mouse)
• C Gérard, C Hubeau, O Carnet, M Bellefroid, NE Sounni, S Blacher, G Bendavid, M Moser, R Fässler, A Noel, D Cataldo, N Rocks Microenvironment-derived ADAM28 prevents cancer dissemination Oncotarget, 2018-12-14;9(98):37185-37199. 2018-12-14 [PMID: 30647853] (Bioassay, Mouse)
• C Konopacki, Y Pritykin, Y Rubtsov, CS Leslie, AY Rudensky Transcription factor Foxp1 regulates Foxp3 chromatin binding and coordinates regulatory T cell function Nat. Immunol., 2019-01-14;20(2):232-242. 2019-01-14 [PMID: 30643266] (Bioassay, Mouse)
• SK Martin, S Fitter, N El Khawank, RH Grose, CR Walkley, LE Purton, MA Ruegg, MN Hall, S Gronthos, ACW Zannettino mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis Sci Rep, 2018-09-28;8(1):14501. 2018-09-28 [PMID: 30266921] (Bioassay, Mouse)
• RR Ricardo-Go, SJ Van Dyken, C Schneider, J Lee, JC Nussbaum, HE Liang, D Vaka, WL Eckalbar, AB Molofsky, DJ Erle, RM Locksley Tissue signals imprint ILC2 identity with anticipatory function Nat. Immunol., 2018-09-10;0(0):. 2018-09-10 [PMID: 30201992] (Bioassay, Mouse)
• S Kojo, N Yasmin, S Muroi, M Tenno, I Taniuchi Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene Nat Commun, 2018-09-05;9(1):3593. 2018-09-05 [PMID: 30185787] (Stimulation, Mouse)
• KM Reeder, CW Dunaway, JP Blackburn, Z Yu, S Matalon, AT Hastie, EJ Ampleford, DA Meyers, C Steele The common ?-chain cytokine IL-7 promotes immunopathogenesis during fungal asthma Mucosal Immunol, 2018-06-15;11(5):1352-1362. 2018-06-15 [PMID: 29907867] (In Vivo, Mouse)
• Y Ouchi, A Patil, Y Tamura, H Nishimasu, A Negishi, SK Paul, N Takemura, T Satoh, Y Kimura, M Kurachi, O Nureki, K Nakai, H Kiyono, S Uematsu Generation of tumor antigen-specific murine CD8+ T cells with enhanced anti-tumor activity via highly efficient CRISPR/Cas9 genome editing Int. Immunol., 2018-04-03;30(4):141-154. 2018-04-03 [PMID: 29617862] (Bioassay, Mouse)
• A Seki, S Rutz Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells J. Exp. Med., 2018-02-07;0(0):. 2018-02-07 [PMID: 29436394] (Cell Culture, Mouse)
• M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms Mucosal Immunol, 2017-12-20;11(3):615-626. 2017-12-20 [PMID: 29346348] (Bioassay, Mouse)
• K Dai, Y Huang, Z Chen, X Sun, L Yang, Y Jiang Kbtbd2 inhibits the cytotoxic activity of immortalized NK cells through down-regulating mTOR signaling in a mouse hepatocellular carcinoma model Eur. J. Immunol., 2018-01-29;0(0):. 2018-01-29 [PMID: 29331106] (Bioassay, Mouse)
• SSM Lee-Sayer, N Maeshima, MN Dougan, A Dahiya, AA Arif, M Dosanjh, CA Maxwell, P Johnson Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells Eur. J. Immunol., 2018-01-19;0(0):. 2018-01-19 [PMID: 29315518] (Bioassay, Mouse)
• P Tong, A Granato, T Zuo, N Chaudhary, A Zuiani, SS Han, R Donthula, A Shrestha, D Sen, JM Magee, MP Gallagher, CE van der Po, MC Carroll, DR Wesemann IgH isotype-specific B cell receptor expression influences B cell fate Proc. Natl. Acad. Sci. U.S.A., 2017-09-18;0(0):. 2017-09-18 [PMID: 28923960] (Bioassay, Mouse)
• A Wallrapp, SJ Riesenfeld, PR Burkett, RE Abdulnour, J Nyman, D Dionne, M Hofree, MS Cuoco, C Rodman, D Farouq, BJ Haas, TL Tickle, JJ Trombetta, P Baral, CSN Klose, T Mahlakõiv, D Artis, O Rozenblatt, IM Chiu, BD Levy, MS Kowalczyk, A Regev, VK Kuchroo The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation Nature, 2017-09-13;549(7672):351-356. 2017-09-13 [PMID: 28902842] (Bioassay, Mouse)
• M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice J. Immunol., 2017-07-12;0(0):. 2017-07-12 [PMID: 28701507] (Bioassay, Mouse)
• G Rodríguez-, J Hauer, A Martín-Lor, D Schäfer, C Bartenhage, I García-Ram, F Auer, I Gonzalez-H, L Ruiz-Roca, M Gombert, V Okpanyi, U Fischer, C Chen, M Dugas, S Bhatia, RM Linka, M Garcia-Suq, MV Rascón-Tri, A Garcia-San, O Blanco, MB Garcia-Cen, FJ Garcia-Cri, C Cobaleda, D Alonso-Lóp, J De Las Riv, M Müschen, C Vicente-Du, I Sánchez-Ga, A Borkhardt Infection exposure promotes ETV6-RUNX1 precursor B cell leukemia via impaired H3K4 demethylases Cancer Res., 2017-06-19;0(0):. 2017-06-19 [PMID: 28630052] (Bioassay, Mouse)
• HF Moffett, ANR Cartwright, HJ Kim, J Godec, J Pyrdol, T Äijö, GJ Martinez, A Rao, J Lu, TR Golub, H Cantor, AH Sharpe, CD Novina, KW Wucherpfen The microRNA miR-31 inhibits CD8(+) T cell function in chronic viral infection Nat. Immunol., 2017-05-22;0(0):. 2017-05-22 [PMID: 28530712] (Bioassay, Mouse)
• S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017-04-10;14(5):531-538. 2017-04-10 [PMID: 28394335] (Bioassay, Mouse)
• MJ de Bruijn, J Rip, EK van der Pl, LW van Greuni, VT Ta, LP Kil, AW Langerak, GF Rimmelzwaa, W Ellmeier, RW Hendriks, OB Corneth Distinct and Overlapping Functions of TEC Kinase and BTK in B Cell Receptor Signaling J. Immunol, 2017-03-08;0(0):. 2017-03-08 [PMID: 28275136] (Bioassay, Mouse)
• A Itoh-Nakad, M Matsumoto, H Kato, J Sasaki, Y Uehara, Y Sato, R Ebina-Shib, M Morooka, R Funayama, K Nakayama, K Ochiai, A Muto, K Igarashi A Bach2-Cebp Gene Regulatory Network for the Commitment of Multipotent Hematopoietic Progenitors Cell Rep, 2017-03-07;18(10):2401-2414. 2017-03-07 [PMID: 28273455] (Bioassay, Mouse)
• Jakob von Moltke Leukotrienes provide an NFAT-dependent signal that synergizes with IL-33 to activate ILC2s J. Exp. Med, 2016-12-23;0(0):. 2016-12-23 [PMID: 28011865] (Bioassay, Mouse)
• The non-canonical Wnt receptor Ryk regulates hematopoietic stem cell repopulation in part by controlling proliferation and apoptosis Cell Death Dis, 2016-11-24;7(11):e2479. 2016-11-24 [PMID: 27882948] (Bioassay, Mouse)
• L Zhao, RL Frock, Z Du, J Hu, L Chen, MS Krangel, FW Alt Orientation-specific RAG activity in chromosomal loop domains contributes to Tcrd V(D)J recombination during T cell development J. Exp. Med, 2016-08-15;213(9):1921-36. 2016-08-15 [PMID: 27526713] (Bioassay, Mouse)
• Sande O, Karim A, Li Q, Ding X, Harding C, Rojas R, Boom W Mannose-Capped Lipoarabinomannan from Mycobacterium tuberculosis Induces CD4+ T Cell Anergy via GRAIL. J Immunol, 2015-12-14;196(2):691-702. 2015-12-14 [PMID: 26667170] (Bioassay, Mouse)
• Tamehiro N, Oda H, Shirai M, Suzuki H Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint. PLoS ONE, 2015-06-26;10(6):e0131047. 2015-06-26 [PMID: 26114424] (Bioassay, Mouse)
• Lubeck B, Lapinski P, Oliver J, Ksionda O, Parada L, Zhu Y, Maillard I, Chiang M, Roose J, King P Cutting Edge: Codeletion of the Ras GTPase-Activating Proteins (RasGAPs) Neurofibromin 1 and p120 RasGAP in T Cells Results in the Development of T Cell Acute Lymphoblastic Leukemia. J Immunol, 2015-05-22;195(1):31-5. 2015-05-22 [PMID: 26002977] (Bioassay, Mouse)
• Hao B, Naik A, Watanabe A, Tanaka H, Chen L, Richards H, Kondo M, Taniuchi I, Kohwi Y, Kohwi-Shigematsu T, Krangel M An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development. J Exp Med, 2015-04-06;212(5):809-24. 2015-04-06 [PMID: 25847946] (Bioassay, Mouse)
• Gandhapudi S, Tan C, Marino J, Taylor A, Pack C, Gaikwad J, Van De Wiele C, Wren J, Teague T IL-18 acts in synergy with IL-7 to promote ex vivo expansion of T lymphoid progenitor cells. J Immunol, 2015-03-16;194(8):3820-8. 2015-03-16 [PMID: 25780034] (Bioassay, Mouse)
• Gerby B, Tremblay C, Tremblay M, Rojas-Sutterlin S, Herblot S, Hebert J, Sauvageau G, Lemieux S, Lecuyer E, Veiga D, Hoang T SCL, LMO1 and Notch1 reprogram thymocytes into self-renewing cells. PLoS Genet, 2014-12-18;10(12):e1004768. 2014-12-18 [PMID: 25522233] (Bioassay, Mouse)
• Bando J, Liang H, Locksley R Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine. Nat Immunol, 2014-12-15;16(2):153-60. 2014-12-15 [PMID: 25501629] (Bioassay, Mouse)
• Marcais A, Cherfils-Vicini J, Viant C, Degouve S, Viel S, Fenis A, Rabilloud J, Mayol K, Tavares A, Bienvenu J, Gangloff Y, Gilson E, Vivier E, Walzer T The metabolic checkpoint kinase mTOR is essential for IL-15 signaling during the development and activation of NK cells. Nat Immunol, 2014-06-29;15(8):749-57. 2014-06-29 [PMID: 24973821] (Bioassay, Mouse)
• Smeets M, DeLuca E, Wall M, Quach J, Chalk A, Deans A, Heierhorst J, Purton L, Izon D, Walkley C The Rothmund-Thomson syndrome helicase RECQL4 is essential for hematopoiesis. J Clin Invest, 2014-06-24;124(8):3551-65. 2014-06-24 [PMID: 24960165] (Bioassay, Mouse)
• Liu C, Wang H, Yu S, Jin R, Tang H, Liu Y, Ge Q, Sun X, Zhang Y Id1 expression promotes T regulatory cell differentiation by facilitating TCR costimulation. J Immunol, 2014-06-11;193(2):663-72. 2014-06-11 [PMID: 24920844] (Bioassay, Mouse)
• Green M, Vicente-Duenas C, Romero-Camarero I, Long Liu C, Dai B, Gonzalez-Herrero I, Garcia-Ramirez I, Alonso-Escudero E, Iqbal J, Chan W, Campos-Sanchez E, Orfao A, Pintado B, Flores T, Blanco O, Jimenez R, Martinez-Climent J, Criado F, Cenador M, Zhao S, Natkunam Y, Lossos I, Majeti R, Melnick A, Cobaleda C, Alizadeh A, Sanchez-Garcia I Transient expression of Bcl6 is sufficient for oncogenic function and induction of mature B-cell lymphoma. Nat Commun, 2014-06-02;5(0):3904. 2014-06-02 [PMID: 24887457] (Bioassay, Mouse)
• Cecil D, Holt G, Park K, Gad E, Rastetter L, Childs J, Higgins D, Disis M Elimination of IL-10-inducing T-helper epitopes from an IGFBP-2 vaccine ensures potent antitumor activity. Cancer Res, 2014-04-28;74(10):2710-8. 2014-04-28 [PMID: 24778415] (Bioassay, Mouse)
• Wallington-Beddoe C, Powell J, Tong D, Pitson S, Bradstock K, Bendall L Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression. Cancer Res, 2014-03-31;74(10):2803-15. 2014-03-31 [PMID: 24686171] (Bioassay, Mouse)
• Cheadle E, Sheard V, Rothwell D, Bridgeman J, Ashton G, Hanson V, Mansoor A, Hawkins R, Gilham D Differential role of Th1 and Th2 cytokines in autotoxicity driven by CD19-specific second-generation chimeric antigen receptor T cells in a mouse model. J Immunol, 2014-03-12;192(8):3654-65. 2014-03-12 [PMID: 24623129] (Bioassay, Mouse)
• Krutikov K, Zheng Y, Chesney A, Huang X, Vaags A, Evdokimova V, Hough M, Chen E Ectopic TLX1 expression accelerates malignancies in mice deficient in DNA-PK. PLoS ONE, 2014-02-26;9(2):e89649. 2014-02-26 [PMID: 24586935] (Bioassay, Mouse)
• Constantinides , Michael, McDonald , Benjamin, Verhoef , Philip A, Bendelac , Albert A committed precursor to innate lymphoid cells. Nature, 2014-02-09;508(7496):397-401. 2014-02-09 [PMID: 24509713] (Bioassay, Mouse)
• Coffey F, Lee S, Buus T, Lauritsen J, Wong G, Joachims M, Thompson L, Zuniga-Pflucker J, Kappes D, Wiest D The TCR ligand-inducible expression of CD73 marks gammadelta lineage commitment and a metastable intermediate in effector specification. J Exp Med, 2014-02-03;211(2):329-43. 2014-02-03 [PMID: 24493796] (Bioassay, Mouse)
• Nomachi , Akira, Yoshinaga , Masanori, Liu , Jaron, Kanchanawong , Pakorn, Tohyama , Kiyoshi, Thumkeo , Dean, Watanabe , Takeshi, Narumiya , Shuh, Hirata , Takako Moesin controls clathrin-mediated S1PR1 internalization in T cells. PLoS ONE, 2013-12-16;8(12):e82590. 2013-12-16 [PMID: 24358210] (Bioassay, Mouse)
• Nacionales D, Gentile L, Vanzant E, Lopez M, Cuenca A, Cuenca A, Ungaro R, Li Y, Baslanti T, Bihorac A, Moore F, Baker H, Leeuwenburgh C, Moldawer L, Efron P Aged mice are unable to mount an effective myeloid response to sepsis. J Immunol, 2013-12-13;192(2):612-22. 2013-12-13 [PMID: 24337739] (Bioassay, Mouse)
• Bekiaris V, Sedy J, Macauley M, Rhode-Kurnow A, Ware C The inhibitory receptor BTLA controls gammadelta T cell homeostasis and inflammatory responses. Immunity, 2013-12-05;39(6):1082-94. 2013-12-05 [PMID: 24315996] (Bioassay, Mouse)
• Berezhnoy , Alexey, Castro , Iris, Levay , Agata, Malek , Thomas R, Gilboa , Eli Aptamer-targeted inhibition of mTOR in T cells enhances antitumor immunity. J Clin Invest, 2014-01-01;124(1):188-97. 2014-01-01 [PMID: 24292708] (Cell Culture, Mouse)
• Olive V, Sabio E, Bennett M, De Jong C, Biton A, McGann J, Greaney S, Sodir N, Zhou A, Balakrishnan A, Foth M, Luftig M, Goga A, Speed T, Xuan Z, Evan G, Wan Y, Minella A, He L A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis. Elife, 2013-10-15;2(0):e00822. 2013-10-15 [PMID: 24137534] (Bioassay, Mouse)
• Badano M, Camicia G, Lombardi G, Maglioco A, Cabrera G, Costa H, Meiss R, Piazzon I, Nepomnaschy I B-cell lymphopoiesis is regulated by cathepsin L. PLoS ONE, 2013-04-09;8(4):e61347. 2013-04-09 [PMID: 23585893] (Bioassay, Mouse)
• Venigalla , Ram K C, McGuire , Victoria, Clarke , Rosemary, Patterson-Kane , Janet C, Najafov , Ayaz, Toth , Rachel, McCarthy , Pierre C, Simeons , Frederic, Stojanovski , Laste, Arthur , J Simon PDK1 regulates VDJ recombination, cell-cycle exit and survival during B-cell development. EMBO J, 2013-03-05;32(7):1008-22. 2013-03-05 [PMID: 23463102] (Bioassay, Mouse)
• Lee , Baeck-se, Dekker , Joseph D, Lee , Bum-kyu, Iyer , Vishwana, Sleckman , Barry P, Shaffer , Arthur L, Ippolito , Gregory, Tucker , Philip W The BCL11A transcription factor directly activates RAG gene expression and V(D)J recombination. Mol Cell Biol, 2013-02-25;33(9):1768-81. 2013-02-25 [PMID: 23438597] (Bioassay, Mouse)
• Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, Chen W, Stepkowski S A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells. J Immunol, 2013-02-20;190(7):3153-62. 2013-02-20 [PMID: 23427250] (Bioassay, Mouse)
• Purushothaman D, Marcel N, Garg M, Venkataraman R, Sarin A Apoptotic programs are determined during lineage commitment of CD4+ T effectors: selective regulation of T effector-memory apoptosis by inducible nitric oxide synthase. J Immunol, 2012-12-05;190(1):97-105. 2012-12-05 [PMID: 23225886] (Cell Culture, Mouse)
• Michel M, Pang D, Haque S, Potocnik A, Pennington D, Hayday A Interleukin 7 (IL-7) selectively promotes mouse and human IL-17-producing gammadelta cells. Proc Natl Acad Sci U S A, 2012-10-09;109(43):17549-54. 2012-10-09 [PMID: 23047700] (Bioassay, Mouse)
• Sneddon , Julie B, Borowiak , Malgorza, Melton , Douglas Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012-10-07;491(7426):765-8. 2012-10-07 [PMID: 23041930] (Cell Culture, Human)
• Gupta S, Marcel N, Talwar S, Garg M, R I, Perumalsamy L, Sarin A, Shivashankar G Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration. PLoS ONE, 2012-09-05;7(9):e43718. 2012-09-05 [PMID: 22957031] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Johnson RM, Kerr MS, Slaven JE Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract. J. Immunol., 2012-01-11;188(4):1896-904. 2012-01-11 [PMID: 22238459] (Bioassay, Mouse)
• Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J. Immunol., 2011-12-23;188(3):1503-13. 2011-12-23 [PMID: 22198948] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010-09-29;84(24):12703-12. 2010-09-29 [PMID: 20881038] (In Vivo, Mouse)
• Tripathi P, Kurtulus S, Wojciechowski S STAT5 is critical to maintain effector CD8+ T cell responses. J. Immunol., 2010-07-19;185(4):2116-24. 2010-07-19 [PMID: 20644163] (Bioassay, Mouse)
• Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. Nat. Med., 2010-01-10;16(2):191-7. 2010-01-10 [PMID: 20062065] (Bioassay, In Vivo, Mouse)
• Chen X, Fang L, Song S, Guo TB, Liu A, Zhang JZ Thymic regulation of autoimmune disease by accelerated differentiation of Foxp3+ regulatory T cells through IL-7 signaling pathway. J. Immunol., 2009-10-19;183(10):6135-44. 2009-10-19 [PMID: 19841165] (Bioassay, Mouse)
• Wu JY, Purton LE, Rodda SJ, Chen M, Weinstein LS, McMahon AP, Scadden DT, Kronenberg HM Osteoblastic regulation of B lymphopoiesis is mediated by Gs alpha-dependent signaling pathways. Proc. Natl. Acad. Sci. U.S.A., 2008-10-28;105(44):16976-81. 2008-10-28 [PMID: 18957542] (In Vivo, Mouse)
• Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development. J. Immunol., 2008-09-01;181(5):3285-90. 2008-09-01 [PMID: 18714000] (Bioassay, Mouse)
• Bekiaris V, Withers D, Glanville SH, McConnell FM, Parnell SM, Kim MY, Gaspal FM, Jenkinson E, Sweet C, Anderson G, Lane PJ Role of CD30 in B/T segregation in the spleen. J. Immunol., 2007-12-01;179(11):7535-43. 2007-12-01 [PMID: 18025198] (Bioassay, Mouse)
• Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. J. Immunol., 2007-02-15;178(4):2018-27. 2007-02-15 [PMID: 17277105] (Bioassay, Mouse)
• Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells. Blood, 2007-01-16;109(9):3706-12. 2007-01-16 [PMID: 17227831] (Bioassay, Mouse)
• Letterio J, Rudikoff E, Voong N, Bauer SR Transforming growth factor-beta1 sensitivity is altered in Abl-Myc- and Raf-Myc-induced mouse pre-B-cell tumors. Stem Cells, 2006-08-31;24(12):2611-7. 2006-08-31 [PMID: 16945999] (Bioassay, Mouse)
• Lai L, Zeff RA, Goldschneider I A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells. Blood, 2005-11-22;107(5):1776-84. 2005-11-22 [PMID: 16304052] (Bioassay, Mouse)
• Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J. Immunol., 2005-12-01;175(11):7325-31. 2005-12-01 [PMID: 16301638] (ELISA (Standard))
• Yoshihiro Ueda, Motonari Kondo, Garnett Kelsoe Inflammation and the reciprocal production of granulocytes and lymphocytes in bone marrow The Journal of Experimental Medicine 2005-06-06 [PMID: 15939792] (Bioassay, Mouse)
• Endharti AT, Zhou YW, Nakashima I, Suzuki H Galectin-1 supports survival of naive T cells without promoting cell proliferation. Eur. J. Immunol., 2005-01-01;35(1):86-97. 2005-01-01 [PMID: 15593120] (Bioassay, Mouse)
• Alpdogan O, Schmaltz C, Muriglan SJ, Kappel BJ, Perales MA, Rotolo JA, Halm JA, Rich BE, van den Brink MR Administration of interleukin-7 after allogeneic bone marrow transplantation improves immune reconstitution without aggravating graft-versus-host disease. Blood, 2001-10-01;98(7):2256-65. 2001-10-01 [PMID: 11568014] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Il7
• Uniprot:
  • Q544C8()