Recombinant Mouse MIF Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse MIF Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived mouse MIF protein
  Pro2-Ala115
• Accession #: NP_034928
• N-terminal Sequence: Pro2
• Protein/Peptide Type: Recombinant Proteins
• Gene: Mif
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MIF Protein, CF

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Mouse MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (6). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 8). Structure-function studies suggests MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol- to-keto) has been demonstrated and is dependent upon Pro at position #1 (9). Amino acids 3 - 23 have also been shown to be reminescent of a GST glutathione-binding domain (10). MIF has proinflammatory cytokine activity centered on aa’s 49 - 65. On fibroblasts, MIF induces IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release folllowing IFN-gamma activation (11, 12). Mouse MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (13, 14). Mouse MIF is active on human cells, while human MIF is active on mouse cells (12). Mouse MIF is 99%, 84%, 90%, and 90% aa identical to rat, porcine, bovine and human MIF, respectively.

1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12. 
2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1. 
3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791. 
4. Bozza, M. et al. (1995) Genomics 27:412. 
5. Mitchell, R. et al. (1995) J. Immunol. 154:3863. 
6. Flieger, O. et al. (2003) FEBS Lett. 551:78. 
7. Lanahan, A. et al. (1992) Mol. Cell. Biol. 12:3919. 
8. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
9. Stamps, S.L. et al. (2000) Biochemistry 39:9671.
10. Blocki, F.A. et al. (1993) Protein Sci. 2:2095.
11. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
12. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
13. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
14. Meyer-Siegler, K.L. and P.L. Vera  (2005) J. Urology 173:615.

Publications for MIF (1978-MF/CF)(17)

Publications using 1978-MF/CF

• Banks, WA;Hansen, KM;Erickson, MA;Crews, FT; High-mobility group box 1 (HMGB1) crosses the BBB Bidirectionally Brain, behavior, and immunity 2023-05-03 [PMID: 37146655] (In Vivo, Mouse)
• CS Rigazio, N Mariz-Pont, EP Caballero, FN Penas, NB Goren, MH Santamaría, RS Corral Involvement of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor in proinflammatory mechanisms promoting cardiovascular injury mechanisms promoting cardiovascular inflammation tThe combined action of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor increases proinflammatory mediator production by cardiomyocytes and vascular endothelial cells Microbial pathogenesis, 2022-11-12;0(0):105881. 2022-11-12 [PMID: 36379373] (Bioassay, Mouse)
• J Amosse, M Durcin, M Malloci, L Vergori, A Fleury, F Gagnadoux, S Dubois, G Simard, J Boursier, O Hue, MC Martinez, R Andriantsi, S Le Lay Phenotyping of circulating extracellular vesicles (EVs) in obesity identifies large EVs as functional conveyors of Macrophage Migration Inhibitory Factor Mol Metab, 2018-10-09;18(0):134-142. 2018-10-09 [PMID: 30473096] (Bioassay, Mouse)
• N Priego, L Zhu, C Monteiro, M Mulders, D Wasilewski, W Bindeman, L Doglio, L Martínez, E Martínez-S, SRY Cajal, D Megías, E Hernández-, C Blanco-Apa, L Martínez, E Zarzuela, J Muñoz, C Fustero-To, E Pineiro, A Hernández-, L Bertero, V Poli, M Sánchez-Ma, JA Menendez, R Soffietti, J Bosch-Barr, M Valiente STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis Nat. Med., 2018-06-11;0(0):. 2018-06-11 [PMID: 29892069] (Bioassay, Human)
• A Sparkes, P De Baetsel, L Brys, I Cabrito, YG Sterckx, S Schoonoogh, S Muylderman, G Raes, R Bucala, P Vanlandsch, JA Van Ginder, B Stijlemans Novel half-life extended anti-MIF nanobodies protect against endotoxic shock FASEB J., 2018-01-25;0(0):fj201701189R. 2018-01-25 [PMID: 29401625] (Surface Plasmon Resonance (SPR, Mouse)
• CHD7 promotes proliferation of neural stem cells mediated by MIF Mol Brain, 2016-12-13;9(1):96. 2016-12-13 [PMID: 27955690] (Bioassay, Mouse)
• Relationship between elevated soluble CD74 and severity of experimental and clinical ALI/ARDS Sci Rep, 2016-07-22;6(0):30067. 2016-07-22 [PMID: 27444250] (Bioassay, Mouse)
• Jieshi Xie, Le Yang, Lei Tian, Weiyang Li, Lin Yang, Liying Li Macrophage Migration Inhibitor Factor Upregulates MCP-1 Expression in an Autocrine Manner in Hepatocytes during Acute Mouse Liver Injury. Scientific Reports, 2016-06-08;0(0):2045-2322. 2016-06-08 [PMID: 27273604] (Bioassay, Mouse)
• Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion Stem Cells, 2016-05-27;0(0):. 2016-05-27 [PMID: 27145382] (Bioassay, Mouse)
• J Cui, F Zhang, Y Wang, J Liu, X Ming, J Hou, B Lv, S Fang, B Yu Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways Int J Mol Med, 2016-03-29;37(5):1299-309. 2016-03-29 [PMID: 27035848] (Bioassay, Mouse)
• Vujicic M, Senerovic L, Nikolic I, Saksida T, Stosic-Grujicic S, Stojanovic I The critical role of macrophage migration inhibitory factor in insulin activity. Cytokine, 2014-06-04;69(1):39-46. 2014-06-04 [PMID: 25022960] (Bioassay, Mouse)
• Bevilacqua E, Paulesu L, Ferro E, Ietta F, Faria M, Lorenzon A, Costa A, Martucci M Review: putative roles for the macrophage migratory inhibitory factor at the maternal fetal interface. Placenta, 2013-11-01;35(0):S51-6. 2013-11-01 [PMID: 24215782] (Bioassay, Mouse)
• Palud A, Marciniak C, Montaigne D, Marechal X, Ballot C, Hassoun S, Decoster B, Neviere R, Lancel S Macrophage migration inhibitory factor inhibition is deleterious for high-fat diet-induced cardiac dysfunction. PLoS ONE, 2013-03-11;8(3):e58718. 2013-03-11 [PMID: 23536817] (Bioassay, Mouse)
• Cutrullis R, Petray P, Schapachnik E, Sanchez R, Postan M, Gonzalez M, Martin V, Corral R Elevated serum levels of macrophage migration inhibitory factor are associated with progressive chronic cardiomyopathy in patients with Chagas disease. PLoS ONE, 2013-02-22;8(2):e57181. 2013-02-22 [PMID: 23451183] (Bioassay, Mouse)
• Mun S, Won H, Hernandez P, Aguila H, Lee S Deletion of CD74, a putative MIF receptor, in mice enhances osteoclastogenesis and decreases bone mass. J Bone Miner Res, 2013-04-01;28(4):948-59. 2013-04-01 [PMID: 23044992] (Bioassay, Mouse)
• Park SK, Cho MK, Park HK, Lee KH, Lee SJ, Choi SH, Ock MS, Jeong HJ, Lee MH, Yu HS Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment. J. Immunol., 2009-06-01;182(11):6907-14. 2009-06-01 [PMID: 19454687] (In Vivo, Mouse)
• Kariya S, Okano M, Fukushima K, Nomiya S, Kataoka Y, Nomiya R, Akagi H, Nishizaki K Expression of inflammatory mediators in the otitis media induced by Helicobacter pylori antigen in mice. Clin. Exp. Immunol., 2008-08-22;154(1):134-40. 2008-08-22 [PMID: 18727622] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Mif
• Uniprot:
  • NP_034928()