Recombinant Mouse NKG2D Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse NKG2D Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmNKG2D/Fc Chimera at 4 µg/mL (100 µL/well) can bind biotinylated rmRae-1 gamma /Fc Chimera with a linear range of 1.56-100 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse NKG2D/CD314 protein
  

  MD	Human IgG1 (Pro100 - Lys330)	IEGR	Mouse NKG2D (Phe94 - Val232) Accession # Q4FJM0
  N-terminus			C-terminus

• Accession #: Q4FJM0
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Klrk1
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 42.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NKG2D Fc Chimera Protein, CF

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co-stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha , beta , gamma , δ and epsilon . All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta -2 microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1 - 5).

1. Bauer, S. et al. (1999) Science 285:727.
2. Wu, J. et al. (1999) Science 285:730.
3. Diefenbach, A. et al. (2001) Nature 413:165.
4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
5. NKG2D and its Ligands, www.RnDSystems.com.

Publications for NKG2D/CD314 (139-NK)(20)

Publications using 139-NK

• S Badrinath, MO Dellacheri, A Li, S Zheng, X Zhang, M Sobral, JW Pyrdol, KL Smith, Y Lu, S Haag, H Ijaz, F Connor-Str, T Kaisho, G Dranoff, GC Yuan, DJ Mooney, KW Wucherpfen A vaccine targeting resistant tumours by dual T cell plus NK cell attack Nature, 2022-05-25;0(0):. 2022-05-25 [PMID: 35614223] (Bioassay, Human)
• N Alsahafi, J Richard, J Prévost, M Coutu, N Brassard, MS Parsons, DE Kaufmann, M Brockman, A Finzi Impaired downregulation of NKG2D ligands by Nef protein from elite controllers sensitizes HIV-1-infected cells to ADCC J. Virol., 2017-07-27;0(0):. 2017-07-27 [PMID: 28615199] (Flow Cytometry, Human)
• Y Li, M Liu, Z Zuo, J Liu, X Yu, Y Guan, R Zhan, Q Han, J Zhang, R Zhou, R Sun, Z Tian, C Zhang TLR9 Regulates the NF-?B-NLRP3-IL-1? Pathway Negatively in Salmonella-Induced NKG2D-Mediated Intestinal Inflammation J. Immunol., 2017-06-02;0(0):. 2017-06-02 [PMID: 28576980] (Bioassay, Mouse)
• The MICA-129 dimorphism affects NKG2D signaling and outcome of hematopoietic stem cell transplantation EMBO Mol Med, 2015-11-01;7(11):1480-502. 2015-11-01 [PMID: 26483398] (Bioassay, Human)
• VanSeggelen H, Hammill J, Dvorkin-Gheva A, Tantalo D, Kwiecien J, Denisova G, Rabinovich B, Wan Y, Bramson J T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice. Mol Ther, 2015-06-30;23(10):1600-10. 2015-06-30 [PMID: 26122933] (Flow Cytometry, Mouse)
• Tougaard P, Skov S, Pedersen A, Krych L, Nielsen D, Bahl M, Christensen E, Licht T, Poulsen S, Metzdorff S, Hansen A, Hansen C TL1A regulates TCRgammadelta+ intraepithelial lymphocytes and gut microbial composition. Eur J Immunol, 2014-12-23;45(3):865-75. 2014-12-23 [PMID: 25404161] (Flow Cytometry, Mouse)
• Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice. Immunology, 2014-03-01;141(3):401-15. 2014-03-01 [PMID: 24708417] (Bioassay, Mouse)
• Lacotte S, Oldani G, Slits F, Orci L, Rubbia-Brandt L, Morel P, Mentha G, Toso C Alloimmune activation promotes anti-cancer cytotoxicity after rat liver transplantation. PLoS ONE, 2014-03-20;9(3):e91515. 2014-03-20 [PMID: 24651497] (Bioassay, Rat)
• Tietje A, Li J, Yu X, Wei Y MULT1E/mIL-12: a novel bifunctional protein for natural killer cell activation. Gene Ther, 2014-02-27;21(5):468-75. 2014-02-27 [PMID: 24572784] (Bioassay, Mouse)
• Rothe A, Jachimowicz R, Borchmann S, Madlener M, Kessler J, Reiners K, Sauer M, Hansen H, Ullrich R, Chatterjee S, Borchmann P, Yazaki P, Koslowsky T, Engert A, Heukamp L, Hallek M, von Strandmann E The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor activity against colon carcinoma. Int J Cancer, 2013-11-29;134(12):2829-40. 2013-11-29 [PMID: 24242212] (Bioassay, Human)
• Rui Wang, Kannan Natarajan, Maria Jamela R. Revilleza, Lisa F. Boyd, Li Zhi, Huaying Zhao et al. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1 gamma reveals a paradigm for MHC/MHC interaction in immune evasion Proceedings of the National Academy of Sciences 2012-12-18 [PMID: 23169621] (Surface Plasmon Resonance, Mouse)
• Yoshida S, Mohamed RH, Kajikawa M, Koizumi J, Tanaka M, Fugo K, Otsuka N, Maenaka K, Yagita H, Chiba H, Kasahara M Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair. J. Immunol., 2012-03-07;188(8):3972-9. 2012-03-07 [PMID: 22403443] (Flow Cytometry, Mouse)
• Dong Z, Cruz-Munoz ME, Zhong MC, Chen R, Latour S, Veillette A Essential function for SAP family adaptors in the surveillance of hematopoietic cells by natural killer cells. Nat. Immunol., 2009-08-02;10(9):973-80. 2009-08-02 [PMID: 19648922] (Flow Cytometry, Mouse)
• Dibra D, Cutrera JJ, Xia X, Birkenbach MP, Li S Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance. Cancer Res., 2009-06-23;69(13):5505-13. 2009-06-23 [PMID: 19549909] (Flow Cytometry, Human)
• Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J. Mol. Biol., 2008-09-16;384(5):1143-56. 2008-09-16 [PMID: 18809410] (Bioassay, Mouse)
• Kotturi HS, Li J, Branham-O'Connor M, Stickel SL, Yu X, Wagner TE, Wei Y Tumor cells expressing a fusion protein of MULT1 and Fas are rejected in vivo by apoptosis and NK cell activation. Gene Ther., 2008-05-08;15(19):1302-10. 2008-05-08 [PMID: 18463688] (Flow Cytometry, Mouse)
• Unni AM, Bondar T, Medzhitov R Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance. Proc. Natl. Acad. Sci. U.S.A., 2008-01-25;105(5):1686-91. 2008-01-25 [PMID: 18223157] (Flow Cytometry, Mouse)
• Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D. J. Immunol., 2008-02-01;180(3):1678-85. 2008-02-01 [PMID: 18209064] (Bioassay, Surface Plasmon Resonance, Mouse)
• Barber A, Zhang T, DeMars LR, Conejo-Garcia J, Roby KF, Sentman CL Chimeric NKG2D receptor-bearing T cells as immunotherapy for ovarian cancer. Cancer Res., 2007-05-15;67(10):5003-8. 2007-05-15 [PMID: 17510432] (Flow Cytometry, Mouse)
• Zhou H, Luo Y, Lo JF, Kaplan CD, Mizutani M, Mizutani N, Lee JD, Primus FJ, Becker JC, Xiang R, Reisfeld RA DNA-based vaccines activate innate and adaptive antitumor immunity by engaging the NKG2D receptor. Proc. Natl. Acad. Sci. U.S.A., 2005-07-22;102(31):10846-51. 2005-07-22 [PMID: 16040807] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: Klrk1
• Uniprot:
  • Q4FJM0()