Recombinant Mouse Osteopontin/OPN Protein

Recombinant Mouse Osteopontin/OPN Protein (Catalog # 441-OP) supports the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.100-1.20 µg/mL.

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Osteopontin/OPN Protein Summary

• Details of Functionality:

  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.100-1.20 µg/mL.

• Source: Mouse myeloma cell line, NS0-derived mouse Osteopontin/OPN protein
  Leu17-Asn294 (Glu99Gly), with a C-terminal 6-His tag
• Accession #: Q547B5
• N-terminal Sequence: Leu17
• Protein/Peptide Type: Recombinant Proteins
• Gene: Spp1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 31.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30 kDa and a 65 kDa doublet, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Osteopontin/OPN Protein

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Mouse OPN is synthesized as a 294 amino acid (aa) precursor protein with a 16 aa signal peptide and a 278 aa mature protein (3). Mature mouse OPN shares 79% and 64% aa sequence identity with rat and human OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1-4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell ad­hesion and migration (1, 2, 4, 6). Its expression is up-regulat­ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
3. Miyazaki, Y. et al. (1990) J. Biol. Chem. 265:14432.
4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
9. Takafuji, V. et al. (2007) Oncogene 26:6361.
10. Kiefer, F.W. et al. (2010) Diabetes 59:935.

Publications for Osteopontin/OPN (441-OP)(43)

Publications using 441-OP

• Gu, Y;Taifour, T;Bui, T;Zuo, D;Pacis, A;Poirier, A;Attalla, S;Fortier, AM;Sanguin-Gendreau, V;Pan, TC;Papavasiliou, V;Lin, NU;Hughes, ME;Smith, K;Park, M;Tremblay, ML;Chodosh, LA;Jeselsohn, R;Muller, WJ; Osteopontin is a therapeutic target that drives breast cancer recurrence Nature communications 2024-10-24 [PMID: 39448577] (Bioassay, In vivo assay, Transgenic Mouse)
• Mamand, DR;Bazaz, S;Mohammad, DK;Saher, O;Wiklander, OPB;Sadeghi, B;Hassan, M;El-Andaloussi, S;Abedi-Valugerdi, M; Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase International immunopharmacology 2024-02-15 [PMID: 38364741] (Bioassay, Mouse)
• Bourayou, E;Perchet, T;Meunier, S;Bouvier, H;Mailhe, MP;Melanitou, E;Cumano, A;Golub, R; Bone marrow monocytes sustain NK cell-poiesis during non-alcoholic steatohepatitis Cell reports 2024-01-11 [PMID: 38217855] (Bioassay, Mouse, Transgenic Mouse)
• Ji, J;Zheng, S;Liu, Y;Xie, T;Zhu, X;Nie, Y;Shen, Y;Han, X; Increased expression of OPN contributes to idiopathic pulmonary fibrosis and indicates a poor prognosis Journal of translational medicine 2023-09-19 [PMID: 37726818] (In Vivo, Mouse)
• Wang, J;Ocadiz-Ruiz, R;Hall, MS;Bushnell, GG;Orbach, SM;Decker, JT;Raghani, RM;Zhang, Y;Morris, AH;Jeruss, JS;Shea, LD; A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs Nature communications 2023-08-08 [PMID: 37553342] (Bioassay, Mouse)
• Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV; Signalling by senescent melanocytes hyperactivates hair growth Nature 2023-06-01 [PMID: 37344645] (In vivo assay, Transgenic Mouse, Xenograft)
• Andries, L;Kancheva, D;Masin, L;Scheyltjens, I;Van Hove, H;De Vlaminck, K;Bergmans, S;Claes, M;De Groef, L;Moons, L;Movahedi, K; Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons Acta neuropathologica communications 2023-05-24 [PMID: 37226256] (In Vivo, Mouse)
• D Sawaki, Y Zhang, A Mohamadi, M Pini, Z Mezdari, L Lipskaia, S Naushad, L Lamendour, DM Altintas, M Breau, H Liang, M Halfaoui, T Delmont, M Surenaud, D Rousseau, T Yoshimitsu, F Louache, S Adnot, C Henegar, P Gual, G Czibik, G Derumeaux Osteopontin promotes age-related adipose tissue remodeling through senescence-associated macrophage dysfunction JCI Insight, 2023-04-24;8(8):. 2023-04-24 [PMID: 37092554] (In Vivo, Bioassay, Mouse)
• Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer&#039;s disease Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120. 2023-02-02 [PMID: 36730200] (ELISA Capture, Mouse)
• Q Bai, X Wang, H Yan, L Wen, Z Zhou, Y Ye, Y Jing, Y Niu, L Wang, Z Zhang, J Su, T Chang, G Dou, Y Wang, J Sun Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling Journal of personalized medicine, 2023-01-11;13(1):. 2023-01-11 [PMID: 36675807] (Bioassay, Mouse)
• J Chen, P Zeng, L Gong, X Zhang, Z Ling, K Bi, F Shi, K Wang, Q Zhang, J Jiang, Y Zhang, T Uede, EM El-Omar, H Diao Osteopontin Exacerbates High-Fat Diet-Induced Metabolic Disorders in a Microbiome-Dependent Manner MBio, 2022-10-27;0(0):e0253122. 2022-10-27 [PMID: 36300928] (Bioassay, Mouse)
• CL Hong, IS Yu, CH Pai, JS Chen, MS Hsieh, HL Wu, SW Lin, HP Huang CD248 regulates Wnt signaling in pericytes to promote angiogenesis and tumor growth in lung cancer Cancer Research, 2022-10-17;0(0):. 2022-10-17 [PMID: 35950912] (Cell Culture, Human)
• FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure Science Advances, 2022-02-25;8(8):eabm5900. 2022-02-25 [PMID: 35213222] (In Vivo, Mouse)
• S Makishi, T Yamazaki, H Ohshima Osteopontin on the Dental Implant Surface Promotes Direct Osteogenesis in Osseointegration International Journal of Molecular Sciences, 2022-01-18;23(3):. 2022-01-18 [PMID: 35162963] (Bioassay, Mouse)
• D Raineri, G Cappellano, B Vilardo, F Maione, N Clemente, E Canciani, E Boggio, CL Gigliotti, C Monge, C Dianzani, R Boldorini, U Dianzani, A Chiocchett Inducible T-Cell Costimulator Ligand Plays a Dual Role in Melanoma Metastasis upon Binding to Osteopontin or Inducible T-Cell Costimulator Biomedicines, 2021-12-27;10(1):. 2021-12-27 [PMID: 35052731] (Bioassay, Mouse)
• S Yoshida, H Nakagami, H Hayashi, Y Ikeda, J Sun, A Tenma, H Tomioka, T Kawano, M Shimamura, R Morishita, H Rakugi The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice Nat Commun, 2020-05-18;11(1):2482. 2020-05-18 [PMID: 32424156] (Western Blot Control, Mouse)
• KM Herum, A Romaine, A Wang, AO Melleby, ME Strand, J Pacheco, B Braathen, P Dunér, T Tønnessen, IG Lunde, I Sjaastad, C Brakebusch, AD McCulloch, MF Gomez, CR Carlson, G Christense Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin J Am Heart Assoc, 2020-01-31;9(3):e013518. 2020-01-31 [PMID: 32000579] (Cell Culture, Mouse)
• PG Hitscheric, LH Xie, D Del Re, EJ Lee The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models Physiol Rep, 2019-07-01;7(13):e14137. 2019-07-01 [PMID: 31301118] (Cell Culture, Mouse)
• R Rogall, M Rabenstein, S Vay, A Bach, A Pikhovych, J Baermann, M Hoehn, S Couillard-, GR Fink, M Schroeter, MA Rueger Bioluminescence imaging visualizes osteopontin-induced neurogenesis and neuroblast migration in the mouse brain after stroke Stem Cell Res Ther, 2018-07-04;9(1):182. 2018-07-04 [PMID: 29973246] (In Vivo, Mouse)
• Q Zeng, X Luo, M Han, W Liu, H Li Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity EBioMedicine, 2018-06-07;0(0):. 2018-06-07 [PMID: 29885866] (In Vivo, Mouse)
• RM Natoli, H Yu, MC Meislin, P Abbasnia, P Roper, A Vuchkovska, X Xiao, SR Stock, JJ Callaci Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro J Orthop Surg Res, 2018-04-27;13(1):101. 2018-04-27 [PMID: 29699560] (Bioassay, Mouse)
• MB Alvarez, L Xu, P Childress, KA Maupin, SFG Mohamad, B Chitteti, E Himes, DJ Olivos Iii, YH Cheng, SJ Conway, EF Srour, MA Kacena Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis Stem Cells Dev., 2018-05-15;0(0):. 2018-05-15 [PMID: 29631496] (Bioassay, Mouse)
• G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 2018-02-26;0(0):. 2018-02-26 [PMID: 29359350] (In Vivo, Mouse)
• M Quattrocel, J Capote, JC Ohiri, JL Warner, AH Vo, JU Earley, M Hadhazy, AR Demonbreun, MJ Spencer, EM McNally Genetic modifiers of muscular dystrophy act on sarcolemmal resealing and recovery from injury PLoS Genet., 2017-10-24;13(10):e1007070. 2017-10-24 [PMID: 29065150] (In Vivo, Mouse)
• W Wang, P Li, W Li, J Jiang, Y Cui, S Li, Z Wang Osteopontin activates mesenchymal stem cells to repair skin wound PLoS ONE, 2017-09-28;12(9):e0185346. 2017-09-28 [PMID: 28957406] (Bioassay, Mouse)
• AM Paul, D Acharya, L Duty, EA Thompson, L Le, DS Stokic, AA Leis, F Bai Osteopontin facilitates West Nile virus neuroinvasion via neutrophil Trojan horse transport Sci Rep, 2017-07-05;7(1):4722. 2017-07-05 [PMID: 28680095] (Bioassay, Mouse)
• CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway Sci Rep, 2017-01-18;7(0):40183. 2017-01-18 [PMID: 28098144] (Bioassay, Rat)
• Richard P Ciavarra Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep J. Neuroimmunol, 2016-12-29;0(0):. 2016-12-29 [PMID: 28041664] (In Vivo, Mouse)
• Nanoparticle Tracking Analysis for the Enumeration and Characterization of Mineralo-Organic Nanoparticles in Feline Urine PLoS ONE, 2016-12-22;11(12):e0166045. 2016-12-22 [PMID: 28005930] (Bioassay, Feline)
• Y Kamioka, K Takakura, K Sumiyama, M Matsuda Intravital F�rster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli Cancer Sci, 2017-02-01;108(2):226-235. 2017-02-01 [PMID: 27960041] (Western Blot, Mouse)
• Osteopontin in Spontaneous Germinal Centers Inhibits Apoptotic Cell Engulfment and Promotes Anti-Nuclear Antibody Production in Lupus-Prone Mice J Immunol, 2016-08-17;0(0):. 2016-08-17 [PMID: 27534552] (Bioassay, Mouse)
• Yuta Morisaki Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9 Sci Rep, 2016-06-06;6(0):27354. 2016-06-06 [PMID: 27264390] (Bioassay, Mouse)
• Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival. J Biol Chem, 2015-02-17;290(15):9377-86. 2015-02-17 [PMID: 25691576] (Bioassay, Mouse)
• Qi , Qian-Ron, Xie , Qing-Zhe, Liu , Xue-Li, Zhou , Yun Osteopontin is expressed in the mouse uterus during early pregnancy and promotes mouse blastocyst attachment and invasion in vitro. PLoS ONE, 2014-08-18;9(8):e104955. 2014-08-18 [PMID: 25133541] (Bioassay, Mouse)
• Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V Osteopontin expression by CD103- dendritic cells drives intestinal inflammation. Proc Natl Acad Sci U S A, 2014-02-18;111(9):E856-65. 2014-02-18 [PMID: 24550510] (In Vivo, Mouse)
• Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells. Stem Cells, 2014-02-01;32(2):327-37. 2014-02-01 [PMID: 24123709] (Bioassay, Mouse)
• Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26. 2012-03-23 [PMID: 22444159] (Surface Plasmon Resonance, Human)
• Dahiya S, Givvimani S, Bhatnagar S Osteopontin-Stimulated Expression of Matrix Metalloproteinase-9 Causes Cardiomyopathy in the mdx Model of Duchenne Muscular Dystrophy. J. Immunol., 2011-08-01;187(5):2723-31. 2011-08-01 [PMID: 21810612] (In Vivo, Mouse)
• Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease. FASEB J., 2009-08-31;24(1):70-80. 2009-08-31 [PMID: 19720619] (In Vivo, Western Blot, Mouse)
• Marcondes MC, Poling M, Watry DD, Hall D, Fox HS In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression. Cell. Immunol., 2008-08-03;254(1):56-62. 2008-08-03 [PMID: 18678363] (In Vivo, Mouse)
• Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Nat. Med., 2007-04-15;13(5):570-8. 2007-04-15 [PMID: 17435770] (In Vivo, Mouse)
• Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Arthritis Rheum., 2004-08-01;50(8):2685-94. 2004-08-01 [PMID: 15334485] (ELISA (Standard))
• Bruemmer D, Collins AR, Noh G, Wang W, Territo M, Arias-Magallona S, Fishbein MC, Blaschke F, Kintscher U, Graf K, Law RE, Hsueh WA Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice. J. Clin. Invest., 2003-11-01;112(9):1318-31. 2003-11-01 [PMID: 14597759] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Spp1
• Uniprot:
  • Q547B5()