Recombinant Mouse R-Spondin 1 Protein, CF

Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 50‑200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

1 μg/lane of Recombinant Mouse R-Spondin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse R-Spondin 1 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
• Source: E. coli-derived mouse R-Spondin 1 protein
  Ser21-Gly209, with an N-terminal Met
• Accession #: Q9Z132
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Rspo1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse R-Spondin 1 Protein, CF

• Cristin 3
• CRISTIN3
• FLJ40906Roof plate-specific spondin-1
• HRspo1
• roof plate-specific spondin
• RSPO
• RSPO1
• RSpondin 1
• R-Spondin 1
• R-spondin homolog (Xenopus laevis)
• RSPONDIN
• R-spondin1
• R-spondin-1

Background

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
9. Kim, K.-A. et al. (2005) Science 309:1256.
10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
11. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
12. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
13. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.

Publications for R-Spondin 1 (3474-RS)(71)

Publications using 3474-RS

• Chen, C;Centonze, A;Song, Y;Chrisnandy, A;Tika, E;Rezakhani, S;Zahedi, Z;Bouvencourt, G;Dubois, C;Keymeulen, A;Lütolf, M;Sifrim, A;Blanpain, C; Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice Nature communications 2024-12-18 [PMID: 39695111] (Bioassay, Mouse)
• Hentschel, V;Govindarajan, D;Seufferlein, T;Armacki, M; An Adaptable Protocol to Generate a Murine Enteroid-Macrophage Co-Culture System International journal of molecular sciences 2024-07-20 [PMID: 39063188] (Bioassay, Mouse)
• Kubrak, O;Jørgensen, AF;Koyama, T;Lassen, M;Nagy, S;Hald, J;Mazzoni, G;Madsen, D;Hansen, JB;Larsen, MR;Texada, MJ;Hansen, JL;Halberg, KV;Rewitz, K; LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance Nature communications 2024-07-20 [PMID: 39033139] (Bioassay, Mouse)
• Malviya, G;Lannagan, T;Johnson, E;Mackintosh, A;Bielik, R;Peters, A;Soloviev, D;Brown, G;Jackstadt, R;Nixon, C;Gilroy, K;Campbell, A;Sansom, O;Lewis, D; Noninvasive Stratification of Colon Cancer by Multiplex PET Imaging Clinical Cancer Research 2024-03-17 [PMID: 38493804] (Bioassay, Mouse)
• Sakata, N;Yoshimatsu, G;Kawakami, R;Nakano, K;Yamada, T;Yamamura, A;Nagashima, H;Kodama, S; The porcine islet-derived organoid showed the characteristics as pancreatic duct Scientific reports 2024-03-16 [PMID: 38493252] (Bioassay, Porcine)
• Malla, SB;Byrne, RM;Lafarge, MW;Corry, SM;Fisher, NC;Tsantoulis, PK;Mills, ML;Ridgway, RA;Lannagan, TRM;Najumudeen, AK;Gilroy, KL;Amirkhah, R;Maguire, SL;Mulholland, EJ;Belnoue-Davis, HL;Grassi, E;Viviani, M;Rogan, E;Redmond, KL;Sakhnevych, S;McCooey, AJ;Bull, C;Hoey, E;Sinevici, N;Hall, H;Ahmaderaghi, B;Domingo, E;Blake, A;Richman, SD;Isella, C;Miller, C;Bertotti, A;Trusolino, L;Loughrey, MB;Kerr, EM;Tejpar, S;Maughan, TS;Lawler, M;Campbell, AD;Leedham, SJ;Koelzer, VH;Sansom, OJ;Dunne, PD; Pathway level subtyping identifies a slow-cycling biological phenotype associated with poor clinical outcomes in colorectal cancer Nature genetics 2024-02-13 [PMID: 38351382] (Bioassay, Mouse)
• Jiang, Z;Waterbury, QT;Malagola, E;Fu, N;Kim, W;Ochiai, Y;Wu, F;Guha, C;Shawber, CJ;Yan, KS;Wang, TC; Microbial-dependent recruitment of immature myeloid cells promotes intestinal regeneration Cellular and molecular gastroenterology and hepatology 2023-10-26 [PMID: 37898454] (Organoid Culture, Mouse)
• Hung, YH;Capeling, M;Villanueva, JW;Kanke, M;Shanahan, MT;Huang, S;Cubitt, R;Rinaldi, VD;Schimenti, JC;Spence, JR;Sethupathy, P; Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model BMC genomics 2023-10-26 [PMID: 37884859] (Mouse)
• X Huang, T Nero, R Weeraseker, KH Matej, A Hinbest, Z Jiang, RF Lee, L Wu, C Chak, J Nijjer, I Gibaldi, H Yang, N Gamble, WL Ng, SA Malaker, K Sumigray, R Olson, J Yan Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization Nature Communications, 2023-04-13;14(1):2104. 2023-04-13 [PMID: 37055389] (Bioassay, Mouse)
• TT Lemmetyine, EW Viitala, L Wartiovaar, T Kaprio, J Hagström, C Haglund, P Katajisto, TC Wang, E Domènech-M, S Ollila Fibroblast-derived EGF ligand Neuregulin-1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth Disease Models & Mechanisms, 2023-04-03;0(0):. 2023-04-03 [PMID: 36912192] (Bioassay, Mouse)
• E Domènech-M, A Brandt, TT Lemmetyine, L Wartiovaar, TP Mäkelä, S Ollila Tellu: an object detector algorithm for automatic classification of intestinal organoids Disease Models & Mechanisms, 2023-03-13;0(0):. 2023-03-13 [PMID: 36804687] (Bioassay, Mouse)
• JW Villanueva, L Kwong, T Han, SA Martinez, MT Shanahan, M Kanke, LE Dow, CG Danko, P Sethupathy Comprehensive microRNA analysis across genome-edited colorectal cancer organoid models reveals miR-24 as a candidate regulator of cell survival BMC Genomics, 2022-12-01;23(1):792. 2022-12-01 [PMID: 36457077] (Bioassay, Mouse)
• M Viladomiu, M Khounlotha, B Dogan, SF Lima, A Elsaadi, E Cardakli, JG Castellano, C Ng, J Herzog, AA Schoenborn, M Ellermann, B Liu, S Zhang, AS Gulati, RB Sartor, KW Simpson, SM Lipkin, RS Longman Agr2-associated ER stress promotes adherent-invasive E.�coli dysbiosis and triggers CD103+ dendritic cell IL-23-dependent ileocolitis Cell Reports, 2022-11-15;41(7):111637. 2022-11-15 [PMID: 36384110] (Bioassay, Transgenic Mouse)
• K Kumar Mani, Y El-Hakim, TE Branyan, N Samiya, S Pandey, MT Grimaldo, A Habbal, A Wertz, F Sohrabji Intestinal Epithelial Stem Cell Transplants as a Novel Therapy for Cerebrovascular Stroke Brain, Behavior, and Immunity, 2022-10-31;0(0):. 2022-10-31 [PMID: 36328163] (Mouse)
• S Watanabe, S Kobayashi, N Ogasawara, R Okamoto, T Nakamura, M Watanabe, KB Jensen, S Yui Transplantation of intestinal organoids into a mouse model of colitis Nature Protocols, 2022-02-02;0(0):. 2022-02-02 [PMID: 35110738] (Bioassay, Mouse)
• Y Han, A Villarreal, G Gutierrez, Q Nguyen, P Sun, T Wu, B Sui, G Berx, T Brabletz, K Kessenbroc, YA Zeng, K Watanabe, X Dai Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1 Cell Reports, 2022-01-11;38(2):110240. 2022-01-11 [PMID: 35021086] (Bioassay, Mouse)
• JR Knight, N Vlahov, DM Gay, RA Ridgway, WJ Faller, C Proud, GR Mallucci, T von der Ha, CM Smales, AE Willis, OJ Sansom Rpl24Bst mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K Elife, 2021-12-13;10(0):. 2021-12-13 [PMID: 34895463] (Bioassay, Mouse)
• M Tarquis-Me, K Scheibner, I González-G, A Bastidas-P, M Sterr, J Jaki, S Schirge, C García-Các, H Lickert, M Bakhti Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas International Journal of Molecular Sciences, 2021-11-20;22(22):. 2021-11-20 [PMID: 34830411] (Bioassay, Mouse)
• SR Taylor, S Ramsamooj, RJ Liang, A Katti, R Pozovskiy, N Vasan, SK Hwang, N Nahiyaan, NJ Francoeur, EM Schatoff, JL Johnson, MA Shah, AJ Dannenberg, RP Sebra, LE Dow, LC Cantley, KY Rhee, MD Goncalves Dietary fructose improves intestinal cell survival and nutrient absorption Nature, 2021-08-18;0(0):. 2021-08-18 [PMID: 34408323] (Bioassay, Mouse)
• JDG Leach, N Vlahov, P Tsantoulis, RA Ridgway, DJ Flanagan, K Gilroy, N Sphyris, EG Vázquez, DF Vincent, WJ Faller, MC Hodder, A Raven, S Fey, AK Najumudeen, D Strathdee, C Nixon, M Hughes, W Clark, R Shaw, S:CORT con, SR van Hooff, DJ Huels, JP Medema, ST Barry, MC Frame, A Unciti-Bro, SJ Leedham, GJ Inman, R Jackstadt, BJ Thompson, AD Campbell, S Tejpar, OJ Sansom Oncogenic BRAF, unrestrained by TGF&amp;beta-receptor signalling, drives right-sided colonic tumorigenesis Nature Communications, 2021-06-08;12(1):3464. 2021-06-08 [PMID: 34103493] (Bioassay, Mouse)
• A Böttcher, M Büttner, S Tritschler, M Sterr, A Aliluev, L Oppenlände, I Burtscher, S Sass, M Irmler, J Beckers, C Ziegenhain, W Enard, AC Schamberge, FM Verhamme, O Eickelberg, FJ Theis, H Lickert Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates Nature Cell Biology, 2021-01-04;23(1):23-31. 2021-01-04 [PMID: 33398177] (Bioassay, Transgenic Mouse)
• AJ Bilotta, C Ma, W Yang, Y Yu, Y Yu, X Zhao, Z Zhou, S Yao, SM Dann, Y Cong Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair Cell Mol Gastroenterol Hepatol, 2020-11-22;0(0):. 2020-11-22 [PMID: 33238220] (Cell Culture, Mouse)
• S Claudinot, JI Sakabe, H Oshima, C Gonneau, T Mitsiadis, D Littman, P Bonfanti, G Martens, M Nicolas, A Rochat, Y Barrandon Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence Nat Commun, 2020-11-06;11(1):5645. 2020-11-06 [PMID: 33159086] (Cell Culture, Mouse)
• Y Li, M Chen, J Hu, R Sheng, Q Lin, X He, M Guo Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/&amp;beta-Catenin Signaling Cell Stem Cell, 2020-10-13;0(0):. 2020-10-13 [PMID: 33053374] (Bioassay, Mouse)
• RT Zwiggelaar, HT Lindholm, M Fosslie, M Terndrup P, Y Ohta, A Díez-Sánch, M Martín-Alo, J Ostrop, M Matano, N Parmar, E Kvaløy, RR Spanjers, K Nazmi, M Rye, F Drabløs, C Arrowsmith, J Arne Dahl, KB Jensen, T Sato, MJ Oudhoff LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium Science Advances, 2020-09-11;6(37):. 2020-09-11 [PMID: 32917713] (Bioassay, Mouse)
• X Zhang, S Bandyopadh, LP Araujo, K Tong, J Flores, D Laubitz, Y Zhao, G Yap, J Wang, Q Zou, R Ferraris, L Zhang, W Hu, EM Bonder, PR Kiela, R Coffey, MP Verzi, II Ivanov, N Gao Elevating EGFR-MAPK program by a nonconventional Cdc42 enhances intestinal epithelial survival and regeneration JCI Insight, 2020-08-20;5(16):. 2020-08-20 [PMID: 32686657] (Bioassay, Human)
• T Han, S Goswami, Y Hu, F Tang, MP Zafra, C Murphy, Z Cao, JT Poirier, E Khurana, O Elemento, JF Hechtman, K Ganesh, R Yaeger, LE Dow Lineage reversion drives WNT independence in intestinal cancer Cancer Discov, 2020-06-16;0(0):. 2020-06-16 [PMID: 32546576] (Bioassay, Mouse)
• M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs PLoS ONE, 2020-03-11;15(3):e0229445. 2020-03-11 [PMID: 32160239] (IHC Control, Mouse)
• T Xing, LJ Benderman, S Sabu, J Parker, J Yang, Q Lu, L Ding, YH Chen Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation Cell Mol Gastroenterol Hepatol, 2019-12-23;0(0):. 2019-12-23 [PMID: 31874254] (Organoid Culture, Mouse)
• AP Singh, YH Hung, MT Shanahan, M Kanke, A Bonfini, MK Dame, M Biraud, BCE Peck, OO Oyesola, JM Freund, RL Cubitt, EG Curry, LM Gonzalez, GA Bewick, ED Tait-Wojno, NA Kurpios, S Ding, JR Spence, CM Dekaney, N Buchon, P Sethupathy Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner Cell Mol Gastroenterol Hepatol, 2019-11-19;0(0):. 2019-11-19 [PMID: 31756561] (Tissue Culture, Mouse)
• X Wang, Y Han, M Wang, C Bo, Z Zhang, L Xu, W Liu, H Wang Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro Biomed Res Int, 2019-10-07;2019(0):7878906. 2019-10-07 [PMID: 31687397] (Tissue Culture, Mouse)
• T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals Nat Commun, 2019-10-11;10(1):4647. 2019-10-11 [PMID: 31604927] (Bioassay, Mouse)
• EM Schatoff, S Goswami, MP Zafra, M Foronda, M Shusterman, BI Leach, A Katti, BJ Diaz, LE Dow Distinct CRC-associated APC mutations dictate response to Tankyrase inhibition Cancer Discov, 2019-07-23;0(0):. 2019-07-23 [PMID: 31337618] (Tissue Culture, Mouse)
• KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer Molecular determinants of WNT9b responsiveness in nephron progenitor cells PLoS ONE, 2019-04-12;14(4):e0215139. 2019-04-12 [PMID: 30978219] (Bioassay, Mouse)
• Z Meng, T Gwag, Y Sui, SH Park, X Zhou, C Zhou The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling JCI Insight, 2019-02-07;4(3):. 2019-02-07 [PMID: 30728326] (Bioassay, Mouse)
• F Chen, W Yang, X Huang, AT Cao, AJ Bilotta, Y Xiao, M Sun, L Chen, C Ma, X Liu, CG Liu, S Yao, SM Dann, Z Liu, Y Cong Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis J. Immunol., 2018-08-31;0(0):. 2018-08-31 [PMID: 30171165] (Bioassay, Mouse)
• NR Smith, JR Swain, PS Davies, AC Gallagher, MS Parappilly, CZ Beach, PR Streeter, IA Williamson, ST Magness, MH Wong Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5-�and Bmi1-Expressing Intestinal Cell Populations Cell Mol Gastroenterol Hepatol, 2018-03-10;6(1):79-96. 2018-03-10 [PMID: 29928673] (Bioassay, Mouse)
• M Boyd, M Thodberg, M Vitezic, J Bornholdt, K Vitting-Se, Y Chen, M Coskun, Y Li, BZS Lo, P Klausen, P Jan Schwei, AG Pedersen, N Rapin, K Skovgaard, K Dahlgaard, R Andersson, TB Terkelsen, B Lilje, JT Troelsen, AM Petersen, KB Jensen, I Gögenur, P Thielsen, JB Seidelin, OH Nielsen, JT Bjerrum, A Sandelin Characterization of the enhancer and promoter landscape of inflammatory bowel disease from human colon biopsies Nat Commun, 2018-04-25;9(1):1661. 2018-04-25 [PMID: 29695774] (Bioassay, Human)
• MJ Kim, B Xia, HN Suh, SH Lee, S Jun, EM Lien, J Zhang, K Chen, JI Park PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis Dev. Cell, 2018-03-12;44(5):582-596.e4. 2018-03-12 [PMID: 29533773] (Bioassay, Mouse)
• T Takahashi, A Shiraishi, J Murata The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice Int J Mol Sci, 2018-03-05;19(3):. 2018-03-05 [PMID: 29510587] (Bioassay, Mouse)
• Y Saito, N Onishi, H Takami, R Seishima, H Inoue, Y Hirata, K Kameyama, K Tsuchihash, E Sugihara, S Uchino, K Ito, H Kawakubo, H Takeuchi, Y Kitagawa, H Saya, O Nagano Development of a functional thyroid model based on an organoid culture system Biochem. Biophys. Res. Commun., 2018-02-19;0(0):. 2018-02-19 [PMID: 29470983] (Bioassay, Mouse)
• S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2017-12-14;22(1):35-49.e7. 2017-12-14 [PMID: 29249464] (Bioassay, Mouse)
• MA Scavuzzo, D Yang, M Borowiak Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells Sci Rep, 2017-09-07;7(1):10810. 2017-09-07 [PMID: 28883507] (Bioassay, Mouse)
• EC Moorefield, SF Andres, RE Blue, L Van Landeg, AT Mah, MA Santoro, S Ding Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells Aging (Albany NY), 2017-08-29;9(8):1898-1915. 2017-08-29 [PMID: 28854151] (Bioassay, Mouse)
• PJ Attayek, AA Ahmad, Y Wang, I Williamson, CE Sims, ST Magness, NL Allbritton In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment PLoS ONE, 2016-04-21;11(4):e0153795. 2016-04-21 [PMID: 27100890] (Bioassay, Mouse)
• Morrison G, Scognamiglio R, Trumpp A, Smith A Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification. EMBO J, 2015-12-16;35(3):356-68. 2015-12-16 [PMID: 26675138] (Bioassay, Mouse)
• Huels D, Ridgway R, Radulescu S, Leushacke M, Campbell A, Biswas S, Leedham S, Serra S, Chetty R, Moreaux G, Parry L, Matthews J, Song F, Hedley A, Kalna G, Ceteci F, Reed K, Meniel V, Maguire A, Doyle B, Soderberg O, Barker N, Watson A, Larue L, Clarke A, Sansom O E-cadherin can limit the transforming properties of activating beta-catenin mutations. EMBO J, 2015-08-03;34(18):2321-33. 2015-08-03 [PMID: 26240067] (Bioassay, Mouse)
• Calderon-Gierszal E, Prins G Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure. PLoS ONE, 2015-07-29;10(7):e0133238. 2015-07-29 [PMID: 26222054] (Bioassay, Human)
• Barrett C, Reddy V, Short S, Motley A, Lintel M, Bradley A, Freeman T, Vallance J, Ning W, Parang B, Poindexter S, Fingleton B, Chen X, Washington M, Wilson K, Shroyer N, Hill K, Burk R, Williams C Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage. J Clin Invest, 2015-06-08;125(7):2646-60. 2015-06-08 [PMID: 26053663] (Bioassay, Mouse)
• Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis. PLoS ONE, 2015-04-24;10(4):e0124213. 2015-04-24 [PMID: 25910078] (Bioassay, Mouse)
• Moon C, Zhang W, Ren A, Arora K, Sinha C, Yarlagadda S, Woodrooffe K, Schuetz J, Valasani K, de Jonge H, Shanmukhappa S, Shata M, Buddington R, Parthasarathi K, Naren A Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea. J Biol Chem, 2015-03-11;290(18):11246-57. 2015-03-11 [PMID: 25762723] (Bioassay, Mouse)
• Dow , Lukas E, Fisher , Jonathan, O'Rourke , Kevin P, Muley , Ashlesha, Kastenhuber , Edward R, Livshits , Geulah, Tschaharganeh , Darjus F, Socci , Nicholas, Lowe , Scott W Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol, 2015-02-18;33(4):390-4. 2015-02-18 [PMID: 25690852] (Bioassay, Mouse)
• Lopez-Arribillaga E, Rodilla V, Pellegrinet L, Guiu J, Iglesias M, Roman A, Gutarra S, Gonzalez S, Munoz-Canoves P, Fernandez-Salguero P, Radtke F, Bigas A, Espinosa L Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch. Development, 2014-12-05;142(1):41-50. 2014-12-05 [PMID: 25480918] (Bioassay, Mouse)
• McElroy S, Castle S, Bernard J, Almohazey D, Hunter C, Bell B, Al Alam D, Wang L, Ford H, Frey M The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis. Am J Pathol, 2014-09-09;184(10):2768-78. 2014-09-09 [PMID: 25216938] (Bioassay, Mouse)
• Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon. Genes Dev, 2014-08-15;28(16):1752-7. 2014-08-15 [PMID: 25128495] (Bioassay, Mouse)
• Capaldo C, Farkas A, Hilgarth R, Krug S, Wolf M, Benedik J, Fromm M, Koval M, Parkos C, Nusrat A Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins. Mol Biol Cell, 2014-07-16;25(18):2710-9. 2014-07-16 [PMID: 25031428] (Bioassay, Mouse)
• Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nat Commun, 2014-06-24;2(0):4172. 2014-06-24 [PMID: 24960204] (Bioassay, Mouse)
• Mah , Amanda T, Van Landeghem , Lauriann, Gavin , Hannah E, Magness , Scott T, Lund , P Kay Impact of diet-induced obesity on intestinal stem cells: hyperproliferation but impaired intrinsic function that requires insulin/IGF1. Endocrinology, 2014-06-10;155(9):3302-14. 2014-06-10 [PMID: 24914941] (Bioassay, Mouse)
• Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol, 2014-06-06;193(1):439-51. 2014-06-06 [PMID: 24907348] (Bioassay, Mouse)
• Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36. 2014-05-13 [PMID: 24825889] (Bioassay, Mouse)
• Shi F, Hu L, Jacques B, Mulvaney J, Dabdoub A, Edge A beta-Catenin is required for hair-cell differentiation in the cochlea. J Neurosci, 2014-05-07;34(19):6470-9. 2014-05-07 [PMID: 24806673] (Bioassay, Mouse)
• Takemura N, Kawasaki T, Kunisawa J, Sato S, Lamichhane A, Kobiyama K, Aoshi T, Ito J, Mizuguchi K, Karuppuchamy T, Matsunaga K, Miyatake S, Mori N, Tsujimura T, Satoh T, Kumagai Y, Kawai T, Standley D, Ishii K, Kiyono H, Akira S, Uematsu S Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome. Nat Commun, 2014-03-18;5(0):3492. 2014-03-18 [PMID: 24637670] (Bioassay, Mouse)
• Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy. PLoS ONE, 2014-02-26;9(2):e89881. 2014-02-26 [PMID: 24587096] (Bioassay, Mouse)
• Matsumoto , Shinji, Fujii , Shinsuke, Sato , Akira, Ibuka , Souji, Kagawa , Yoshinor, Ishii , Masaru, Kikuchi , Akira A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J, 2014-02-20;33(7):702-18. 2014-02-20 [PMID: 24562386] (Bioassay, Mouse)
• Li , Ji-Yao, Chai , Biaoxin, Zhang , Weizhen, Fritze , Danielle, Zhang , Chao, Mulholland , Michael LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats. Endocrinology, 2013-11-26;155(2):429-40. 2013-11-26 [PMID: 24280058] (In Vivo, Mouse)
• Jin , Liang, Feng , Tao, Zerda , Ricardo, Chen , Ching-Ch, Riggs , Arthur D, Ku , Hsun Ter In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice. Stem Cells Dev, 2014-01-04;23(8):899-909. 2014-01-04 [PMID: 24261600] (Bioassay, Mouse)
• Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, Stanley E, Ramsay R CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response. PLoS ONE, 2013-02-22;8(2):e56951. 2013-02-22 [PMID: 23451116] (Bioassay, Mouse)
• Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel. Proc Natl Acad Sci U S A, 2013-02-19;110(10):3907-12. 2013-02-19 [PMID: 23431132] (Bioassay, Mouse)
• Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, Clarke A, Dale T In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system. Oncogene, 2012-04-02;32(7):883-93. 2012-04-02 [PMID: 22469981] (Bioassay, Mouse)
• Wong , Vivian W, Stange , Daniel E, Page , Mahalia, Buczacki , Simon, Wabik , Agnieszk, Itami , Satoshi, van de Wetering , Marc, Poulsom , Richard, Wright , Nicholas, Trotter , Matthew, Watt , Fiona M, Winton , Doug J, Clevers , Hans, Jensen , Kim B Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol, 2012-03-04;14(4):401-8. 2012-03-04 [PMID: 22388892] (Cell Culture, Mouse)
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Bioinformatics

• Gene Symbol: Rspo1
• Uniprot:
  • Q9Z132()