Recombinant Mouse Semaphorin 3D Fc Chimera Protein, CF

RecombinantMouse Semaphorin-3D (Catalog # 9386‑S3/CF) binds HUVEC human umbilical vein endothelialcells in a dose-dependent manner. TheED50 for this effect is1.5 -9 µg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 3D Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 1.5 -9 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Semaphorin 3D protein
  

  Mouse Semaphorin 3D (Ser38-Glu738) Accession # Q8BH34	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q8BH34
• N-terminal Sequence: Ser38
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 97-113 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 3D Fc Chimera Protein, CF

• (semaphorin) 3D
• coll-2
• Collapsin 2
• MGC39708
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• SEMA3D
• Semaphorin 3D
• Semaphorin-3D
• Sema-Z2

Background

Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2).  Class 3 semaphorins are secreted molecules, and contain a common domain structure. The mouse Sema3D domains consist of a 488 aa Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4).  Within these domains, mouse Sema3D shares 94% and 98% aa sequence identity with human and rat Sema3D, respectively.  Semaphorin 3D is a secreted guidance molecule involved in angiogenesis and neuronal development (5).  It induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (6, 7). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (8). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (8).  Semaphorin 3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).

1. Sabag, A. et al. (2012) PLoS One. 7:e42912.
2. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
3. Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
4. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
5. Binch, A. et al. (2015) Oncotarget. 6:18338.
6. Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
7. Wolman, M.A. et al. (2007) J. Neurobiology. 27: 9653-63.
8. Aghajanian, H. et al. (2015) Nature Commun. 7:12038.

Publications for SEMA3D (9386-S3/CF)(1)

Publication using 9386-S3/CF

• V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B J. Neurosci., 2019-06-10;0(0):. 2019-06-10 [PMID: 31182634] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • Q8BH34()