Red Blood Cell (RBC) Lysis Buffer

Red Blood Cell (RBC) Lysis Buffer [NBP2-29442] - data shows before (left) and after (right) human whole blood cells were lysed using the Red Blood Cell (RBC) lysis buffer. Image from verified customer review.

• Reactivity: Hu, Mu
• Applications: Func

Red Blood Cell (RBC) Lysis Buffer Summary

• Immunogen: The Red Blood Cell (RBC) Lysis Buffer is for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic issues such as spleen and human peripheral blood. This buffer contains ammonium chloride, which lyses red cells with minimal effect on lymphocytes. Nucleated red blood cells are not effectively lysed with ammonium chloride. RBC lysis is not necessary when working with mouse thymus and lymph node.

Applications/Dilutions

• Dilutions:
  • Functional reported in scientific literature (PMID 24371146)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24371146). Human reactivity reported in scientific literature (PMID: 24080766)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Dilute 10X RBC Lysis Buffer to 1X in deionized water before use (1 ml of 10X RBC lysis buffer with 9 ml of deionized water). Bring to room temperature prior to use.

Alternate Names for Red Blood Cell (RBC) Lysis Buffer

• RBC
• Red Blood Cell (RBC) Lysis Buffer (10X)
• Red Blood Cell (RBC) Lysis Buffer
• Red Blood Cell (RBC) Lysis
• Red Blood Cell (RBC)
• Red Blood Cell

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Red Blood Cell (RBC) Lysis Buffer (NBP2-29442)(5)

Publications using NBP2-29442

• Gao L, Zhu L, Shen C et al. The transdermal cream of Formestane anti-breast cancer by controlling PI3K-Akt pathway and the tumor immune microenvironment Frontiers in immunology 2023-03-28 [PMID: 37056757] (Flow Cytometry, Rat)
• Ishida Y, Kimura A, Nosaka M et al. Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination Int. J. Legal Med. 2015-04-07 [PMID: 25845667] (Func)
• Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014-05-07 [PMID: 24804954] (Red Blood Cell Lysis, Mouse)
  
  Details:
  Mouse splenocytes, human PBMC, Jurkat, EL4: Fig 5. GADPH was detected at ~36 kDa.
• Sturrock A, Mir-Kasimov M, Baker J et al. Key role of microRNA in the regulation of Granulocyte-macrophage colony stimulating factor expression in murine alveolar epithelial cells during oxidative stress. J Biol Chem 2013-12-26 [PMID: 24371146] (Func, Mouse)
• Gorocs Zoltan, Ling Yuye, Yu Meng Dai et al. Giga-pixel fluorescent imaging over an ultra-large field-of-view using a flatbed scanner. Lab Chip. 2013-11-21 [PMID: 24080766] (Human)

Review for Red Blood Cell (RBC) Lysis Buffer (NBP2-29442) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 09/11/2015

Summary

• Application: Flow Cytometry
• Sample Tested: human whole blood
• Species: Human
• Lot: 022113

FAQs for Red Blood Cell (RBC) Lysis Buffer (NBP2-29442). (Showing 1 - 1 of 1 FAQs).

1. Regarding the following product, please let us know the concentration of ammonium chloride.
   • The concentration for ammonium chloride in NBP2-29442 (Red Blood Cell (RBC) Lysis Buffer) is 8%, replied by the Imgenex lab