RED Phosphatase Substrate Kit

Immunohistochemistry: RED Phosphatase Substrate Kit [KA0566] - This kit demonstrates glial fibrillary acidic protein in retinal glial cells.

• Applications: IHC
• Suitable Sample Type: Biological Samples

RED Phosphatase Substrate Kit Summary

• Description: Quality control test: IHC demonstration data
  
  Materials Required but Not Supplied: Primary antibody; AP labeled reagents; Isopropyl alcohol; Xylene based mounting media; 0.1 M Tris HCl (See Preparation of reagent); 1 M Citric Acid Free Acid (See Preparation of reagent). RED Phosphatase Substrate Kit contains reagents for the detection of alkaline phosphatase-labeled conjugates. RED reagents form a brilliant scarlet reaction product that is stable in organic solvents. Sections reacted with RED reagents can be dehydrated through graded alcohols and xylene, then mounted in xylene-based mounting media for permanent documentation. The hematoxylin counterstain, Contrast BLUE, provides sharp contrast and excelllent nuclear detail.
• Kit Type: Substrate Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

The application of antibodies and other reagents such as avidin, streptavidin, etc., covalently coupled to calf intestine alkaline phosphatase in immunohistology is well documented. The procedure described in this insert employs a simultaneous capture azo-dye technique, providing the research laboratory a method for precise localization of alkaline phosphatase labeled reagents. Primary aryl amines, when reacted with alkyl nitrites in acid media, form azo compounds. These react with substituted naphthols to produce highly chromogenic insoluble dyes. In this procedure the phosphate ester of 6-bromo-2-hydroxy-3-naphthoic acid (Buffered Substrate Solution) is employed as substrate. Enzymatic hydrolysis, in the presence of hexazotized triaminotrimethyltriphenylmethane (PhThaloRED Solution) results in the formation of a brilliant red reaction product. Endogenous enzyme is eliminated by incorporation of levamisol. It should be noted that a levamisole-resistant alkaline phosphatase has been demonstrated in some malignant cells from serous effusions. Additional blocking measures may be required.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

FAQs for RED Phosphatase Kit (KA0566). (Showing 1 - 1 of 1 FAQ).

1. Does Novus have some multistaining kits for IHC?. Our customer needs to stain his IHC with with different reactivities in the same tissue and he is looking for kits with this function.
   • For the staining of multiple antigens, your customer may employ immunoenzymatic or immunofluorescence staining methods. For immunoenzymatic staining, we offer several chromogen, and you may suggest the following list of kits to the customer:, Reliable DAB Peroxidase Assay Kit 500 Slides (KA0562), RED Phosphatase Assay Kit 1000 Slides (KA0566), Real Blue Peroxidase Assay Kit 500 Slides (KA0565), ORANGE Peroxidase Assay Kit 1000 Slides (KA0563), BLUE Phosphatase Assay Kit 1000 Slides (KA0567). For immunofluorescence staining, we offer several types of secondary antibodies (conjugated to different immunofluorescent dyes) which may be used for labeling different antigens. The customer may chose the options from our secondary antibodies.