Reliable DAB Peroxidase Substrate Kit

Immunohistochemistry-Paraffin: Reliable DAB Peroxidase Assay Kit [KA0562] - IHC-P staining of smooth muscle actin in skin tissue using chromogen - Reliable DAB Peroxidase Assay Kit (KA0562)

Product Discontinued

Reliable DAB Peroxidase Substrate Kit Summary

• Description: Quality control test: IHC demonstration data
  
  Materials Required but Not Supplied: Primary antibody; Peroxidase blocking solution or H2O2; HRP-labeled secondary antibody or streptavidin; Contrast BLUE or hematoxylin; Isopropyl alcohol; Mounting media; 0.1 M Tris-HCl or PBS HCl or PBS (See Reagent Preparation). ReliableDAB Peroxidase Substrate Kits ReliableDAB Peroxidase Substrate kit overcomes this disadvantage and can remain ready-to-use for 2 weeks after preparation.
• Kit Type: Substrate Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

The application of antibodies and other proteins covalently coupled to horseradish peroxidase (HRP) in immunohistology is well documented. It is the most frequently used label for immunohistologic techniques. In the presence of peroxide, HRP catalyzes the oxidation of phenols, naphthols, diamines, aminophenols, indophenols, etc. forming chromogenic products visible by light microscopy. Most commonly employed are 3-amino-9-ethylcarbazole, p-phenylenediamine/catechol, 4-chlorol-napthol and diaminobenzidine (DAB). Although a suspected carcinogen, DAB is the most widely accepted donor substrate for peroxidase immunohistochemistry, since it provides a reaction product insoluble in alcohols and Xylene. The oxidation of DAB results in formation of a free radical intermediate which polymerizes to form a brown product. DAB may be employed for demonstration of endogenous peroxidase and catalase activity, cytochrome oxidase, cupric ferrocyanide, and hemoproteins such as hemoglobin, myoglobin, and cytochrome c. Treatment of the DAB product with osmium, silver, cobalt or nickel will intensify final color. Reaction with osmium tetraoxide results in an electron opaque osmium black useful for ultrastructure research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

FAQs for Reliable DAB Peroxidase Kit (KA0562). (Showing 1 - 1 of 1 FAQ).

1. Does Novus have some multistaining kits for IHC?. Our customer needs to stain his IHC with with different reactivities in the same tissue and he is looking for kits with this function.
   • For the staining of multiple antigens, your customer may employ immunoenzymatic or immunofluorescence staining methods. For immunoenzymatic staining, we offer several chromogen, and you may suggest the following list of kits to the customer:, Reliable DAB Peroxidase Assay Kit 500 Slides (KA0562), RED Phosphatase Assay Kit 1000 Slides (KA0566), Real Blue Peroxidase Assay Kit 500 Slides (KA0565), ORANGE Peroxidase Assay Kit 1000 Slides (KA0563), BLUE Phosphatase Assay Kit 1000 Slides (KA0567). For immunofluorescence staining, we offer several types of secondary antibodies (conjugated to different immunofluorescent dyes) which may be used for labeling different antigens. The customer may chose the options from our secondary antibodies.