Renin Antibody [Unconjugated] - 1

Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli and tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

RLCs invade the glomerulus during EC model.A. Intraglomerular RLCs tagged by beta -gal, but negative for renin appear in the regenerative phase of the EC model (day 7). Representative confocal microscopy images for day 0 and day 7 of beta -gal/renin co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. The channels for green ( beta -gal) and red (renin) fluorescent signals in the dashed square on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm; B. Representative 3D reconstruction of glomeruli and beta -gal labelled RLCs (blue) on day 0 and day 7 of the EC model. The mesangial cell marker alpha 8-integrin (red) was used to visualize the glomeruli. Scale bars correspond to 20 μm; C. Quantification of glomeruli with tufts containing beta -gal expressing RLCs in the regenerative phase of the EC model (day 7). Data are presented as mean ± SEM, n = 5/10 for day 0 (baseline) /day 7, respectively. n.d.—not detectable; D. The intraglomerular RLCs observed during the EC model are not of hematopoietic origin. Representative confocal microscopy images for day 0 and day 7 of beta -gal/CD45 (hematopoietic marker) co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. The channels for green ( beta -gal) and red (CD45) fluorescent signals in the dashed square on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC, IP, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Renin Antibody [Unconjugated] - 1 Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Renin 1
  Leu22-Arg402
  Accession # P06281
• Specificity: Detects mouse Renin 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant rat Renin is observed, and less than 0.2% cross-reactivity with recombinant human Renin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ren1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture:Mouse Renin 1 Antibody (Catalog # AF4277)
  Standard: Recombinant Mouse Renin (Catalog # 4277-AS)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Renin Antibody [Unconjugated] - 1

• angiotensin-forming enzyme
• Angiotensinogenase
• EC 3.4.23
• EC 3.4.23.15
• FLJ10761
• HNFJ2
• Prorenin
• REN
• renin precursor, renal
• Renin

Background

Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin 1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro Renin shares 70% and 85% aa sequence identity with human and rat pro Renin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Renin Antibody (AF4277)(13)

Publications using AF4277

• Mohammad Saleem, Luz Saavedra-Sánchez, Pierina Barturen-Larrea, Jose A. Gomez The Transcription Factor Sox6 Controls Renin Expression during Renal Artery Stenosis Kidney360 5/1/2021 [PMID: 35373064]
• Yatsumu Saito, Naoto Matsumoto, Shuichiro Yamanaka, Takashi Yokoo, Eiji Kobayashi Beneficial Impact of Interspecies Chimeric Renal Organoids Against a Xenogeneic Immune Response Frontiers in Immunology 2/15/2022 [PMID: 35242145]
• Y Funato, D Yamazaki, D Okuzaki, N Yamamoto, H Miki Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure Nature Communications, 2021-06-17;12(1):3683. 2021-06-17 [PMID: 34140503] (Western Blot, Transgenic Mouse)
• BL Phipps, U Suwannasua, J Lucero, NA Mitchell, AK Lund Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice Toxicology reports, 2021-04-15;8(0):846-862. 2021-04-15 [PMID: 33948438] (IHC, Mouse)
• K Miyauchi, T Nakai, S Saito, T Yamamoto, K Sato, K Kato, M Nezu, M Miyazaki, S Ito, M Yamamoto, N Suzuki Renal interstitial fibroblasts coproduce erythropoietin and renin under anaemic conditions EBioMedicine, 2021-01-25;64(0):103209. 2021-01-25 [PMID: 33508746] (IHC, Western Blot, Mouse)
• Loïc Le Gal, Maxime Pellegrin, Tania Santoro, Lucia Mazzolai, Armin Kurtz, Paolo Meda et al. Connexin37-Dependent Mechanisms Selectively Contribute to Modulate Angiotensin II -Mediated Hypertension Journal of the American Heart Association 4/16/2019 [PMID: 30943815]
• NM Novielli-K, M Jelen, K Barr, LJ Delailo, Q Feng, BE Isakson, R Gros, DW Laird Ablation of both Cx40 and Panx1 results in similar cardiovascular phenotypes exhibited in Cx40 knockout mice Biosci. Rep., 2019-02-27;0(0):. 2019-02-27 [PMID: 30745457] (IHC-P, Mouse)
• Christian Karger, Katharina Machura, André Schneider, Christian Hugo, Vladimir T. Todorov, Armin Kurtz COX-2-derived PGE2 triggers hyperplastic renin expression and hyperreninemia in aldosterone synthase-deficient mice Pflügers Archiv - European Journal of Physiology 7/1/2018 [PMID: 29455241]
• Peter Lachmann, Linda Hickmann, Anne Steglich, Moath Al-Mekhlafi, Michael Gerlach, Niels Jetschin et al. Interference with Gs alpha -Coupled Receptor Signaling in Renin-Producing Cells Leads to Renal Endothelial Damage Journal of the American Society of Nephrology 12/1/2017 [PMID: 28775003]
• Liming Wang, Yonggang Sha, Jingyi Bai, William Eisner, Matthew A. Sparks, Anne F. Buckley et al. Podocyte-specific knockout of cyclooxygenase 2 exacerbates diabetic kidney disease American Journal of Physiology-Renal Physiology 8/1/2017 [PMID: 28490532]
• Komers R, Xu B, Fu Y, McClelland A, Kantharidis P, Mittal A, Cohen H, Cohen D Transcriptome-based analysis of kidney gene expression changes associated with diabetes in OVE26 mice, in the presence and absence of losartan treatment. PLoS ONE, 2014-05-14;9(5):e96987. 2014-05-14 [PMID: 24827579] (Western Blot, Mouse)
• Mayer S, Roeser M, Lachmann P Chicken Ovalbumin Upstream Promoter Transcription Factor II Regulates Renin Gene Expression. J. Biol. Chem., 2012-05-29;287(29):24483-91. 2012-05-29 [PMID: 22645148] (Western Blot, Mouse)
• Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 2010-10-21;285(53):41935-46. 2010-10-21 [PMID: 20966072] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Ren1
• Uniprot:
  • P06281()