Rheb Antibody (2C11) - Azide and BSA Free

Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in NIH/3T3.

Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in PC-12.

Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in Raw 264.7.

Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in Jurkat.

ELISA: Rheb Antibody (2C11) [H00006009-M01] - Detection limit for recombinant GST tagged RHEB is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rheb Antibody (2C11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RHEB (AAH16155, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM
• Specificity: RHEB - Ras homolog enriched in brain (2C11)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHEB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rheb Antibody (2C11) - Azide and BSA Free

• GTP-binding protein Rheb
• MGC111559
• Ras homolog enriched in brainRHEB2Ras homolog enriched in brain 2
• Rheb
• Rheb2

Background

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rheb Antibody (H00006009-M01)(9)

Publications using H00006009-M01

• Gianluca F, Sandra M, Anna H et al. Brain-enriched RagB isoforms regulate the dynamics of mTORC1 activity through GATOR1 inhibition. Nat Cell Biol. 2022-09-12 [PMID: 36097071]
• Yanghao Z, Xin Z, Kun-Liang G et al. Rheb regulates nuclear mTORC1 activity independent of farnesylation. Cell Chem Biol. 2022-03-15 [PMID: 35294906]
• Jewell J, Fu V, Hong A et al. GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. Elife. 2019-05-21 [PMID: 31112131]
• Deng L, Chen L, Zhao L et al. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation. Cell Res 2018-12-04 [PMID: 30514904]
• Hoxhaj G, Hughes-Hallett J, Timson RC et al. The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels. Cell Rep 2017-10-31 [PMID: 29091770]
• Tian Q, Gromov P, Clement JH et al. RHEB1 insufficiency in aged male mice is associated with stress-induced seizures. Geroscience 2017-09-10 [PMID: 28891034]
• Jacobs BL, McNally RM, Kim KJ et al. Identification of mechanically regulated phosphorylation sites on tuberin (TSC2) that control mechanistic target of rapamycin (mTOR) signaling. J Biol Chem 2017-03-13 [PMID: 28289099]
• Agarwal S, Bell CM, Taylor SM, Moran RG. p53 Deletion or Hot-spot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res. 2015-09-18 [PMID: 26385560]
• Menon S, Dibble CC, Talbott G et al. Spatial Control of the TSC Complex Integrates Insulin and Nutrient Regulation of mTORC1 at the Lysosome. Cell. 2014-02-13 [PMID: 24529379]

Bioinformatics

• Gene Symbol: RHEB
• Entrez:
  • 6009(Human)
• Uniprot:
  • AAH16155(Human)