RhoJ Antibody (1E4) - Azide and BSA Free

Western Blot: RhoJ Antibody (1E4) [H00057381-M01] - RHOJ expression in HepG2 ( Cat # L019V1 ).

Immunocytochemistry/Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01] - Analysis of monoclonal antibody to RHOJ on HeLa cell. Antibody concentration 60 ug/ml.

ELISA: RhoJ Antibody (1E4) [H00057381-M01] - Detection limit for recombinant GST tagged RHOJ is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clone: 1E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RhoJ Antibody (1E4) - Azide and BSA Free Summary

• Immunogen: RHOJ (AAH62575, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSCCSII
• Specificity: RHOJ - ras homolog gene family, member J
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOJ
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RhoJ Antibody (1E4) - Azide and BSA Free

• ARHJTC10B
• FLJ14445
• ras homolog gene family, member J
• RASL7B
• Ras-like protein family member 7B
• RAS-like, family 7, member B
• RHOI
• rho-related GTP-binding protein RhoJ
• Tc10-like GTP-binding protein
• TC10-like Rho GTPase
• TCLMGC34777

Background

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RhoJ Antibody (H00057381-M01)(6)

Publications using H00057381-M01

• Sohail J, Jose O, Linh V et al. Structure-based design of CDC42 effector interaction inhibitors for the treatment of cancer. Cell Rep. 2022-04-05 [PMID: 35385746]
• Mei W, Chengfei Z, Qian Z et al. RhoJ facilitates angiogenesis in glioblastoma via JNK/VEGFR2 mediated activation of PAK and ERK signaling pathways. Int J Biol Sci. 2022-01-01 [PMID: 35173528]
• Liu S, Li H, Xia L et al. Anti-RhoJ antibody functionalized Au@I nanoparticles as CT-guided tumor vessel-targeting radiosensitizers in patient-derived tumor xenograft model. Biomaterials 2017-06-23 [PMID: 28666098]
• Kim C, Yang H, Park I et al. Rho GTPase RhoJ is Associated with Gastric Cancer Progression and Metastasis. Journal of Cancer 2016-07-08 [PMID: 27471571]
• Ho H, Aruri J, Kapadia R et al. RhoJ and Pak Kinases Regulate Melanoma Chemoresistance by Suppressing Pathways that Sense DNA Damage. Cancer Res. 2012-09-12 [PMID: 22971344]
• Fukushima Y, Okada M, Kataoka H et al. Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice. J Clin Invest. 2011-04-18 [PMID: 21505259]

Bioinformatics

• Gene Symbol: RHOJ
• Entrez:
  • 57381(Human)
• OMIM:
  • 607653(Human)
• Uniprot:
  • AAH62575(Human)