RhoJ Antibody (OTI1D7)

Western Blot: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ.

Immunocytochemistry/Immunofluorescence: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Human breast tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Human liver tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Adenocarcinoma of Human breast tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Human pancreas tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Human prostate tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Carcinoma of Human liver tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Human Kidney tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Carcinoma of Human kidney tissue.

Immunohistochemistry: RhoJ Antibody (OTI1D7) [NBP2-45590] - Analysis of Adenocarcinoma of Human ovary tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI1D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RhoJ Antibody (OTI1D7) Summary

• Immunogen: Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOJ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RhoJ Antibody (OTI1D7)

• ARHJTC10B
• FLJ14445
• ras homolog gene family, member J
• RASL7B
• Ras-like protein family member 7B
• RAS-like, family 7, member B
• RHOI
• rho-related GTP-binding protein RhoJ
• Tc10-like GTP-binding protein
• TC10-like Rho GTPase
• TCLMGC34777

Background

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOJ