Recombinant Human Ribosomal Protein S6/RPS6 GST (N-Term) Protein

Recombinant Human Ribosomal Protein S6/RPS6 Protein [H00006194-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human Ribosomal Protein S6/RPS6 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-249 of Human RPS6
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: RPS6
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence
  • Protein Array
  • Western Blot
• Theoretical MW: 53.13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Ribosomal Protein S6/RPS6 GST (N-Term) Protein

• Phosphoprotein NP33, 40S ribosomal protein S6
• pS6
• Ribosomal Protein S6
• RPS6
• S6

Background

RPS6( AAH00524, 1 a.a. - 250 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Ribosomal Protein S6/RPS6 Recombinant Protein (H00006194-P01)(4)

Publications using H00006194-P01

• Kamila M, Samuel R, Rachael M et al. SILAC kinase screen identifies potential MASTL substrates. Sci Rep. 2022-06-22 [PMID: 35732702]
• YH Park, EF Remmers, W Lee, AK Ombrello, LK Chung, Z Shilei, DL Stone, MI Ivanov, NA Loeven, KS Barron, P Hoffmann, M Nehrebecky, YZ Akkaya-Ulu, E Sag, B Balci-Peyn, I Aksentijev, A Gül, CN Rotimi, H Chen, JB Bliska, S Ozen, DL Kastner, D Shriner, JJ Chae Ancient familial Mediterranean fever mutations in human pyrin and resistance to Yersinia pestis Nat. Immunol., 2020-06-29;0(0):. 2020-06-29 [PMID: 32601469]
• Nam K, Yi S, Nam G et al. Identification of a novel S6K1 inhibitor, rosmarinic acid methyl ester, for treating cisplatin-resistant cervical cancer. BMC Cancer. 2019-08-06 [PMID: 31387554]
• Lee HO, Mustafa A, Hudes GR, Kruger WD. Hydroxychloroquine Destabilizes Phospho-S6 in Human Renal Carcinoma Cells. PLoS One. 2015-07-02 [PMID: 26134285]

Bioinformatics

• Gene Symbol: RPS6