RIN1 Antibody (27G2) - BSA Free

Western Blot: RIN1 Antibody (27G2) [NBP2-66642] - Western blot analysis using Rin antibody (Cat. No. NBP2-66642) on 293 cells expressing HA-tagged Rit (1) and HA-tagged Rin (2).

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 27G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

RIN1 Antibody (27G2) - BSA Free Summary

• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full-length recombinant human Rin protein and mouse myeloma cells.
• Specificity: Does not cross-react with Rit protein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIN1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol (pH 7.5)
• Preservative: 3mM Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for RIN1 Antibody (27G2) - BSA Free

• Ras and Rab interactor 1
• ras inhibitor 1
• Ras inhibitor JC99
• ras inhibitor RIN1
• Ras interaction/interference protein 1

Background

Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIN1
• Uniprot:
  • Q13671(Human)