RNASEH2B Antibody

Western Blot: RNASEH2B Antibody [NBP1-90865] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RNASEH2B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunocytochemistry/Immunofluorescence: RNASEH2B Antibody [NBP1-90865] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: RNASEH2B Antibody [NBP1-90865] - Staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.

Western Blot: RNASEH2B Antibody [NBP1-90865] - Analysis in human cell line MOLT-4.

Product Discontinued

RNASEH2B Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VVDNVFPNCILLLKLPGLEKLLHHVTEEKGNPEIDNKKYYKYSKEKTLKWLEKKVNQTVAALKTNNVNVSSRVQSTAFFSGDQASTDKEEDYIRYAH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNASEH2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RNASEH2B Antibody

• ribonuclease H2, subunit B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNASEH2B