RND1 Antibody (OTI1C3)

Western Blot: RND1 Antibody (1C3) [NBP2-03000] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RND1.

Immunocytochemistry/Immunofluorescence: RND1 Antibody (1C3) [NBP2-03000] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RND1.

Flow Cytometry: RND1 Antibody (1C3) [NBP2-03000] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RND1 antibody, and then analyzed by flow cytometry.

Flow Cytometry: RND1 Antibody (1C3) [NBP2-03000] - Analysis of Hela cells, using anti-RND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI1C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.36 mg/ml

RND1 Antibody (OTI1C3) Summary

• Immunogen: Full length human recombinant protein of human RND1 (NP_055285) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RND1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 25.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.36 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RND1 Antibody (OTI1C3)

• ARHS
• GTP-binding protein
• ras homolog gene family, member S
• Rho family GTPase 1FLJ42294
• RHO6
• rho-related GTP-binding protein Rho6
• RHOS
• Rnd1

Background

Members of the Rho GTPase family, such as RND1, regulate the organization of the actin cytoskeleton in response to extracellular growth factors (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RND1
• Entrez:
  • 27289(Human)