ROCK1 Antibody (464) - Azide and BSA Free

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464].

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000.

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464].

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 (NBP2-42918) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 464
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ROCK1 Antibody (464) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROCK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for ROCK1 Antibody (464) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• MGC131603
• MGC43611
• p160 ROCK-1
• p160ROCK
• p160-ROCK
• PRO0435
• Renal carcinoma antigen NY-REN-35
• Rho kinase
• rho-associated protein kinase 1
• Rho-associated, coiled-coil containing protein kinase 1
• Rho-associated, coiled-coil-containing protein kinase 1
• ROCK1
• ROK beta

Background

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ROCK1