RP105/CD180 Antibody (G28-8) - Low Endotoxin - Azide and BSA Free

Flow (Cell Surface): RP105/CD180 Antibody (G28-8) - Low Endotoxin [NBP2-62228] - Surface staining of human peripheral blood cells with anti-CD180 (G28-8) APC.

Flow (Cell Surface): RP105/CD180 Antibody (G28-8) - Low Endotoxin [NBP2-62228] - Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.

• Reactivity: Hu
• Applications: Flow, Func, IP
• Clone: G28-8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

RP105/CD180 Antibody (G28-8) - Low Endotoxin - Azide and BSA Free Summary

• Additional Information: Clone G28-8 was used by HLDA to establish CD designation.
• Immunogen: Human tonsillar B cells
• Specificity: The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of RP105/CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD180
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2 ug/ml
  • Functional
  • Immunoprecipitation 1:10-1:500
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2 um filter sterilized
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RP105/CD180 Antibody (G28-8) - Low Endotoxin - Azide and BSA Free

• CD180 antigenMGC126233
• CD180 molecule
• CD180
• Ly64
• LY64MGC126234
• Ly78
• Lymphocyte antigen 64
• lymphocyte antigen-64, radioprotective, 105kDa
• Radioprotective 105 kDa protein
• RP105
• RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD180