Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

Immunocytochemistry/Immunofluorescence: Ryanodine Receptor 2 Antibody (C3-33) [NBP2-80143] - ICC/IF analysis of rat brain hippocampus tissue shows that Ryanodine Receptor 2 Antibody (C3-33) crossreacts with Ryanodine Receptor 2.

Immunocytochemistry/Immunofluorescence: Ryanodine Receptor 2 Antibody (C3-33) [NBP2-80143] - ICC/IF analysis of rat brain hippocampus tissue shows that Ryanodine Receptor 2 Antibody (C3-33) crossreacts with Ryanodine Receptor 2.

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, Fi, Gp, Rb
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: C3-33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ryanodine Receptor 2 Antibody (C3-33) - BSA Free Summary

• Immunogen: Canine cardiac ryanodine receptor (RyR2)
• Localization: Membrane; Multi-pass membrane protein
• Specificity: Detects Ryanodine Receptor 2. Also shows cross-reactivity with Ryanodine Receptor 1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RYR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1 ug/ml
• Application Notes: WB: Detects an approx. 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.
  
  IHC: Staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

• arrhythmogenic right ventricular dysplasia 2
• ARVC2
• ARVD2
• cardiac muscle ryanodine receptor-calcium release channel
• Cardiac muscle-type ryanodine receptor
• cardiac-type ryanodine receptor
• hRYR-2
• islet-type ryanodine receptor
• kidney-type ryanodine receptor
• ryanodine receptor 2 (cardiac)
• Ryanodine Receptor 2
• RyR2
• RYR-2
• type 2 ryanodine receptor
• VTSIP

Background

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ryanodine Receptor 2 Antibody (NBP2-80143)(1)

Publication using NBP2-80143

• Yang HW, Lin CY, Lin FZ et al. Phosphodiesterase-1 inhibitor modulates Ca2+ regulation in sirtuin 1-deficient mouse cardiomyocytes European journal of pharmacology 2021-09-08 [PMID: 34506778] (WB, Mouse)

Bioinformatics

• Gene Symbol: RYR2